马铃薯StRGLG1调控干旱胁迫响应的功能研究

doi:10.16420/j.issn.0513-353x.2024-0960

摘要/Abstract

摘要：

RING DOMAIN LIGASE（RGLG）属于RING型泛素连接酶E3，参与植物生长发育、信号转导与非生物胁迫等进程。为进一步研究StRGLG1基因在马铃薯逆境响应中的功能和调控机制，采用qRT-PCR技术检测马铃薯StRGLG1基因在干旱胁迫下的表达水平；通过遗传转化获得马铃薯StRGLG1过表达植株和干扰表达植株，测定了转基因植株在干旱胁迫前后超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性以及丙二醛（MDA）含量。通过酵母双杂交（Y2H）和双分子荧光互补（BiFC）鉴定并验证了StRGLG1相互作用蛋白。结果表明，干旱胁迫下StRGLG1的过表达植株SOD、POD和CAT等酶活性平均分别上升31.78%、46.87%、51.76%，MDA含量下降23.12%，干扰表达植株与之相反，SOD、POD和CAT平均分别下降15.83%、18.28%、13.78%，MDA含量上升13.30%。证明StRGLG1基因在马铃薯耐旱性调控中发挥了作用。Y2H和BiFC结果发现StRGLG1和StERF53蛋白相互作用。这些结果表明，StRGLG1基因的表达对马铃薯的抗逆性具有一定的作用，研究结果可为StRGLG1调控马铃薯干旱胁迫响应的分子机制提供参考。

关键词: 马铃薯, StRGLG1, 干旱胁迫, 蛋白相互作用

Abstract:

RING DOMAIN LIGASE（RGLG）is a ring-type ubiquitin ligase E3，which is involved in plant growth and development，signal transduction and abiotic stress. To further investigate the function and regulatory mechanism ofStRGLG1gene in potato stress response，qRT-PCR was used to assay the expression level ofStRGLG1gene in potato under drought stress.StRGLG1overexpression and interference expression plants of potato were obtained by genetic transformation. The activities of superoxide dismutase（SOD），catalase（CAT）and peroxidase（POD）and the content of malondialdehyde（MDA）in transgenic plants were measured before and after drought stress. StRGLG1 interacting proteins were identified and validated by yeast two-hybrid（Y2H）and bimolecular fluorescence complementation（BiFC）. The results showed that SOD，POD and CAT activities ofStRGLG1overexpression plants increased by 31.78%，46.87% and 51.76% on average，and MDA content decreased by 23.12%. SOD，POD and CAT decreased by 15.83%，18.28% and 13.78% respectively，while MDA content increased by 13.30% under drought stress. It was proved thatStRGLG1gene played a role in the regulation of potato drought tolerance. Y2H and BiFC results showed that StRGLG1 and StERF53 proteins interact. These findings indicate thatStRGLG1gene expression plays a role in potato stress resistance，and the results can provide references for the molecular mechanism ofStRGLG1regulation of potato drought stress response.

Key words: potato, StRGLG1, drought stress, protein interaction

朱存兰, 范军亮, 王凯彤, 韦孟, 杨亮, 刘昊天, 司怀军, 张宁. 马铃薯StRGLG1调控干旱胁迫响应的功能研究[J]. 园艺学报, 2025, 52(9): 2329-2342.

ZHU Cunlan, FAN Junliang, WANG Kaitong, WEI Meng, YANG Liang, LIU Haotian, SI Huaijun, ZHANG Ning. Function Analysis ofStRGLG1in Regulating Potato to Drought Stress Response[J]. Acta Horticulturae Sinica, 2025, 52(9): 2329-2342.

图/表 7

表1 本研究中所用引物

Table 1 Primers used in this study

基因名称/ID Gene name/ID	引物序列（5′-3′） Primer sequence		用途 Application
pCAMBIA1300‐3 5S‐StRGLG1	F	AGAACACGGGGGACGAGCTCATGGGGAATCAAGAGTCTGC	载体构建 Construction of vector
pCAMBIA1300‐3 5S‐StRGLG1	R	TGCTCACCATGTCGACCTAGCTATATAGTCTTATGCGAGTT
pEGFP-amiR-StRGLG1	I miR-s	gaTATGGAATTTACAGTAGCCTAtctctcttttgtattcc
	II miR-a	gaTAGGCTACTGTAAATTCCATAtcaaagagaatcaatga
	III miR*s	gaTAAGCTACTGTAATTTCCATTtcacaggtcgtgatatg
	IV miR*a	gaAATGGAAATTACAGTAGCTTAtctacatatatattcct
	A	CTGCAAGGCGATTAAGTTGGGTAAC
	B	CTGTTTCCTGTGTGAAATTGTTATCCGC
pGBKT7‐StRGLG1	F	AGGAGGACCTGCATATGATGGGGAATCAAGAGTCTGC
pGBKT7‐StRGLG1	R	GCCGCTGCAGGTCGACCTAGCTATATAGTCTTATGCGAGTT
pGADT7‐StERF53	F	GGAGGCCAGTGAATTATGGCAGCTATGGATTTTTGGAA
pGADT7‐StERF53	R	TTCATCTGCAGCTCGTTATAGGGAGGCCCAATCAATTTCA
pSPYNE-StRGLG1	F	CACGGGGGACTCTAGATGGGGAATCAAGAGTCTGC
pSPYNE-StRGLG1	R	TCCATCCCGGGAGCGGCTATATAGTCTTATGCGAGTTTT
pSPYCE-StERF53	F	CACGGGGGACTCTAGATGGCAGCTATGGATTTTTGGAA
pSPYCE-StERF53	R	TACATCCCGGGAGCGTAGGGAGGCCCAATCAATTTCATGA
StRGLG1	F	ACCACACACGATCAGCATGT	qRT-PCR
StRGLG1	R	GTTGTGTGGCTCGGTACTGA
StERF53	F	CAGACTGCGCATCATGCTTC
StERF53	R	TGTCCCAAGTTGGCTCTTCC
Ef1α	F	GATGGTCAGACCCGTGAACA	内参基因 Reference gene
Ef1α	R	CCTTGGAGTACTTCGGGGTG	内参基因 Reference gene
HYG	F	GCTTCTGCGGGCGATTTGTGT	转基因植株鉴定 Transgenic plant identification
HYG	R	GGTCGCGGAGGCTATGGATGC	转基因植株鉴定 Transgenic plant identification

图1 StRGLG1在马铃薯不同组织中的相对表达水平（A）和干旱胁迫下StRGLG1基因的相对表达量（B） WS1：水分胁迫组1（75% ~ 85%）；WS2：水分胁迫组2（55% ~ 65%）；WS3：水分胁迫组3（35% ~ 45%）；WS4：水分胁迫组4（15% ~ 25%）。不同小写字母表示差异显著（n= 3；P< 0.05），下同

Fig. 1 The relative expression level of StRGLG1 in different potato tissues（A）and the relative expression level of StRGLG1 gene under drought stress（B） WS1：Water stress group 1（75%-85%）；WS2：Water stress group 2（55%-65%）；WS3：Water stress group 3（35%-45%）；WS4：Water stress group 4（15%-25%）. Different lowercase letters indicated significant differences （n=3；P< 0.05）. The same below

图2 转基因植物的获取与鉴定 A、B：愈伤组织；C、D：分化芽；E、F：转基因植株生根和筛选；G、H：转基因植株的PCR检测；I、J：StRGLG1在转基因植株和非转基因植株（NT）中的相对表达量。M：DL 2000分子标记；1：阳性对照质粒；2：非转基因植物的阴性对照；3 ~ 5：转基因株系。A、C、E、G、I：携带重组质粒pCAMBIA1300-35S-StRGLG1的植物转化过程；B、D、F、H、J：携带重组质粒pEGFP-amiR-StRGLG1的植物转化过程

Fig. 2 Acquisition and identification of transgenic plants A，B：Calli；C，D：Differentiated buds；E，F：Rooting and screening of transgenic plants；G，H：PCR detection of transgenic plants；I，J：Relative expression levels ofStRGLG1in transgenic plants and non-transgenic plants（NT）. M：DL 2000 molecular marker；1：Positive control plasmid；2：Negative control of non-transgenic plants；3-5：Transgenic lines. A，C，E，G，I：Plant transformation process carrying recombinant plasmid pCAMBIA1300-35S-StRGLG1；B，D，F，H，J：Plant transformation process carrying recombinant plasmid pEGFP-amiR-StRGLG1

图3 转基因马铃薯在干旱胁迫21 d的表型（A）和生理生化指标的测定（B） *和**分别代表不同株系与非转基因植株之间的显著性差异水平和极显著性差异水平

Fig. 3 Phenotype（A）and determination of physiological and biochemical indexes（B）of transgenic potato at 21 days under drought stress * and ** respectively represent the significant difference levels and extremely significant difference levels between different strains and non-transgenic plants *P< 0.05；**P< 0.01，n= 3

图4 诱饵载体pGBKT7-StRGLG1的毒性检测（A）和诱饵载体pGBKT7-StRGLG1的自激活检测（B） 1，2：阳性对照 pGADT7-RecT + pGBKT7-53；3，4：阴性对照 pGADT7-RecT + pGBKT7-Lam；5，6：试验组pGADT7 + pGBKT7-StRGLG1

Fig. 4 Toxicity detection of bait vector pGBKT7-StRGLG1（A）and self-activation detection of decoy vector pGBKT7-StRGLG1（B） 1，2：Positive control pGADT7-RecT + pGBKT7-53；3，4：Negative control pGADT7-RecT + pGBKT7-Lam；5，6：Experimental group pGADT7 + pGBKT7-StRGLG1

图5 酵母双杂交实验及BiFC验证 A：StRGLG1与StERF53的一对一验证；B：BiFC分析检测本氏烟草中StRGLG1与StERF53的互作

Fig. 5 Yeast two-hybrid experiment and BiFC verification A：StRGLG1 and StERF53 one-on-one validation；B：The interaction between StRGLG1 and StERF53 in tobacco was detected by BiFC analysis

图6 qRT-PCR检测干旱胁迫下非转基因植株和转基因StRGLG1植株叶片中StERF53基因的相对表达量

Fig. 6 The relative expression of StERF53 gene in leaves of non-transgenic plants and transgenic StRGLG1 plants under drought stress was detected by qRT-PCR n= 3；*P< 0.05；**P< 0.01

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