辣椒种质资源疫病抗性鉴定及抗病基因挖掘

doi:10.16420/j.issn.0513-353x.2024-0626

园艺学报 ›› 2025, Vol. 52 ›› Issue (4): 857-871.doi: 10.16420/j.issn.0513-353x.2024-0626

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

辣椒种质资源疫病抗性鉴定及抗病基因挖掘

袁娟伟¹^,², 贾利², 王涵², 严从生², 张其安², 俞飞飞², 甘德芳¹^,^*(), 江海坤²^,^*()

¹ 安徽农业大学园艺学院，合肥 230036
² 安徽省农业科学院蔬菜研究所，园艺作物种质创制及高效栽培安徽省重点实验室，农业农村部园艺作物种质创制与利用重点实验室（省部共建），合肥 230031

收稿日期:2024-12-24 修回日期:2025-03-11 出版日期:2025-05-08 发布日期:2025-04-25
通讯作者:
*E-mail：gandf@ahau.edu.cn
*E-mail：jhk211@163.com
基金资助:
现代农业产业技术体系建设专项资助(CARS-23-G40); 现代农业产业技术体系建设专项资助(CARS-23-G49); 安徽省辣椒良种联合攻关项目; 安徽省科技重大专项(202203a06020030); 安徽省农业科学院青年英才经费项目(QNYC-202121); 安徽省蔬菜产业技术体系专项经费项目(皖农科函[2021]711号); 安徽省自然科学研究重点项目(2023AH051056)

Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources

YUAN Juanwei¹^,², JIA Li², WANG Han², YAN Congsheng², ZHANG Qi’an², YU Feifei², GAN Defang¹^,^*(), JIANG Haikun²^,^*()

¹ College of Horticulture，Anhui Agricultural University，Hefei 230036，China
² Key Laboratory of Germplasm Creation and Utilisation of Horticultural Crops，Co-construction Ministry of Agriculture and Rural Affairs and Anhui Province，Key Laboratory of Genetic Improvement and Hingh-Efficiency Cultivation of Horticultural Crop，Institute of vegetable，Anhui Academy of Agricultural Sciences，Hefei 230031，China

Received:2024-12-24 Revised:2025-03-11 Published:2025-05-08 Online:2025-04-25

摘要/Abstract

摘要：

为筛选辣椒抗病种质并挖掘相关基因，利用12对疫病抗性SSR引物对214份辣椒种质材料进行初筛和复筛，得到多态性好的6对SSR抗性引物和56份疫病抗性辣椒种质。利用辣椒疫霉菌孢子悬浮液接种抗性辣椒种质并统计病情，其中11份为高抗。对接种后不同时期的辣椒茎部进行转录组测序，对差异表达基因进行GO、KEGG富集和WGCNA等分析，共筛选出15个抗病相关候选基因。

关键词: 辣椒, 疫霉菌, SSR分子标记, 转录组, 加权基因共表达网络分析, 候选基因

Abstract:

In order to screen pepper germplasm for disease resistance and explore disease resistance related genes，12 pairs of SSR primers for disease resistance were used for initial and secondary screening of 214 pepper germplasm materials，resulting in 6 pairs of SSR resistant primers with good polymorphism and 56 pepper germplasm with disease resistance. Inoculate resistant pepper germplasm with pepper phytophthora spore suspension and record the disease status，among which 11 were highly resistant. Transcriptome sequencing was performed on pepper stems at different stages after inoculation，and GO，KEGG enrichment，and WGCNA analysis were performed on differentially expressed genes. A total of 15 candidate genes related to disease resistance were screened.

Key words: pepper, Phytophthora capsici, SSR molecular marker, transcriptome, WGCNA, candidate gene

袁娟伟, 贾利, 王涵, 严从生, 张其安, 俞飞飞, 甘德芳, 江海坤. 辣椒种质资源疫病抗性鉴定及抗病基因挖掘[J]. 园艺学报, 2025, 52(4): 857-871.

YUAN Juanwei, JIA Li, WANG Han, YAN Congsheng, ZHANG Qi’an, YU Feifei, GAN Defang, JIANG Haikun. Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources[J]. Acta Horticulturae Sinica, 2025, 52(4): 857-871.

图/表 12

参考文献 38

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引物名称Primer name	正向引物（5′-3′）Forward primer sequence	反向引物（5′-3′）Reverse primer sequence
L0101	AACCAGCAATCCCATGAAAACC	GGGCTTTGGGGAGAATAGTGTG
L0132	ATTTTCCTCCATGCCTTCCTATCA	GTTTAGAAGCCCAGGGAATACTCGTACC
L0311	TGCTGGGAAAGATCTCAAAAGG	ATCAAGGAAGCAAACCAATGC
L0315	CACTGCAACTTGTGTACTACTACCAC	CGCTCTTACTGTTTCAAACTTATCTC
L0338	ACCAACTCTCTCTCGTGTCAACAA	GTTTAGGGTACCTTTTGGTGAAGGC
L0501	CCCGCGAAATCAAGGTAAT	AAAGCTATTGCTACTGGGTTCG
L0503	ATGGAGCCATCTGACAGCAAATTC	GTTTCCGAGCTCGATGAGGATGAACTA
L0505	ATGCAGAAGCCTCATCAATTTGGG	GTTTGTCATCATGATCCTCGTTTTTGG
L0601	AGTGTTGCACGTGTCGAGAAACTA	GTTTAGCCTTCAACGTTGGTTTAGCA
L0710	ATATTGTCACGTGGCTTCTCAACA	GTTTCAATAGATTAGTCATCCGTTGCCA
L0805	ACTTTCCTGCAAAGAGCGTATGAT	GTTTGACCTTTCCTCTTATTTCGTGGGG
OpD04.717	CCATAAGGGTTGGTAAATTTACAAAG	TCGAGAGATAATTCAGATAGTATAATC

引物名称Primer name	正向引物（5′-3′）Forward primer sequence	反向引物（5′-3′）Reverse primer sequence
L0101	AACCAGCAATCCCATGAAAACC	GGGCTTTGGGGAGAATAGTGTG
L0132	ATTTTCCTCCATGCCTTCCTATCA	GTTTAGAAGCCCAGGGAATACTCGTACC
L0311	TGCTGGGAAAGATCTCAAAAGG	ATCAAGGAAGCAAACCAATGC
L0315	CACTGCAACTTGTGTACTACTACCAC	CGCTCTTACTGTTTCAAACTTATCTC
L0338	ACCAACTCTCTCTCGTGTCAACAA	GTTTAGGGTACCTTTTGGTGAAGGC
L0501	CCCGCGAAATCAAGGTAAT	AAAGCTATTGCTACTGGGTTCG
L0503	ATGGAGCCATCTGACAGCAAATTC	GTTTCCGAGCTCGATGAGGATGAACTA
L0505	ATGCAGAAGCCTCATCAATTTGGG	GTTTGTCATCATGATCCTCGTTTTTGG
L0601	AGTGTTGCACGTGTCGAGAAACTA	GTTTAGCCTTCAACGTTGGTTTAGCA
L0710	ATATTGTCACGTGGCTTCTCAACA	GTTTCAATAGATTAGTCATCCGTTGCCA
L0805	ACTTTCCTGCAAAGAGCGTATGAT	GTTTGACCTTTCCTCTTATTTCGTGGGG
OpD04.717	CCATAAGGGTTGGTAAATTTACAAAG	TCGAGAGATAATTCAGATAGTATAATC

基因Gene	正向引物（5′-3′）Forward primer sequence	反向引物（5′-3′）Reverse primer sequence
Can02G020380	CAAGAGATGGTATTATACGAAGT	TGTGATGGACGAAGGATAT
Can05G006970	TATTCTACTGATAATGTCCA	AGTCTCACTAATGTTGTT
Can05G009560	GCGATGAATGGAAATAAC	TTAGGCACAACAATAGTC
Can08G006950	TAGACGATGGCTATAAGT	TTATCACATACCGAGAGT
Can07G001460	GGCAAGTTCAAGAAGACA	CAAGCATTAACAGCATCAC
Can12G034470	TTAGTTACAAGCACTTCTA	GCCACATCAATTATTAGC
Can09G002600	ACTTCTTCTGTTGCTGAT	TTGTGGATGTTGTTGTTG
Can05G018720	ATGAAGTCGTCTGTAGATTA	CCAATTCCACAACTCCTA
NewGene_12037	AGTGGAGGTCTAATAGTG	TGTATCAGAGCAAGGTTA
Can01G018740	CAACACTACAAGCAACAT	TAGCATAACGATTCACTATC
β-actin	AATCAATCCCTCCACCTCTTCACTC	CATCACCAGCAAATCCAGCCTT

基因Gene	正向引物（5′-3′）Forward primer sequence	反向引物（5′-3′）Reverse primer sequence
Can02G020380	CAAGAGATGGTATTATACGAAGT	TGTGATGGACGAAGGATAT
Can05G006970	TATTCTACTGATAATGTCCA	AGTCTCACTAATGTTGTT
Can05G009560	GCGATGAATGGAAATAAC	TTAGGCACAACAATAGTC
Can08G006950	TAGACGATGGCTATAAGT	TTATCACATACCGAGAGT
Can07G001460	GGCAAGTTCAAGAAGACA	CAAGCATTAACAGCATCAC
Can12G034470	TTAGTTACAAGCACTTCTA	GCCACATCAATTATTAGC
Can09G002600	ACTTCTTCTGTTGCTGAT	TTGTGGATGTTGTTGTTG
Can05G018720	ATGAAGTCGTCTGTAGATTA	CCAATTCCACAACTCCTA
NewGene_12037	AGTGGAGGTCTAATAGTG	TGTATCAGAGCAAGGTTA
Can01G018740	CAACACTACAAGCAACAT	TAGCATAACGATTCACTATC
β-actin	AATCAATCCCTCCACCTCTTCACTC	CATCACCAGCAAATCCAGCCTT

基因Gene	正向引物（5′-3′）Forward primer sequence	反向引物（5′-3′）Reverse primer sequence
NewGene_12193	GACCACATACCACTCAAG	TCCACAATCCACAATCAG
Can07G034320	TTGATGTAGACGAAGTGA	CATCTCCTGTAAGCATTG
Can01G023360	TGCTATACAATCTTCAAT	CTATTCAATCTCATCCAT
Can07G002780	ATGACAGACAAGGTAGAT	TTACGGTTAAGAGGAGAA
Can04G014490	GATATGTTGCTTGATGGTGGTGAT	GCTTATCTCTTGCTGCGTGAA
Can04G029410	ATGCTAACTTGGTAACAC	TTCATATTGGTCCTTCTTG
NewGene_13172	ATCCTTACAACAACAACA	TTAGAGGTAGAGGTATGG
Can09G002790	GCACTGGTATTGAAGAAG	AAGATTGAGGTATTGAAGATG
Can10G030550	ATAACATTCCATTCTCCAA	CCTCTTCTTCATCATCAG
Can07G025330	GAGTTCCAGTTGATATGA	GCTGTATCCATTATCTCTA
NewGene_11156	AAGGCTCATTCTATTATCAT	AAGGTAGGTATCTCAAGT
Can06G035780	TCCTTACGAGAACAATCATC	GCAAGAGTCACATTATCCA
Can09G017290	GACAACACACCCTATACT	ACATATCTGGATTCTCTTCA
Can06G036020	CATCTTCTACTCATCCACTTA	ATTCTTGCTCAATGTTCAC
Can08G028150	GGTAATTGGAGAGGACAA	AGACATCAGTTGGAAGTT
β-actin	AATCAATCCCTCCACCTCTTCACTC	CATCACCAGCAAATCCAGCCTT

辣椒种质资源疫病抗性鉴定及抗病基因挖掘

Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources

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品种 Cultivar	果形 Fruit shape	病情指数 Disease index	抗性评价 Resistance evaluation	发病率/% Morbidity
H14-205	长牛角形Long bovine horn shape	4.44	R	11.00
V06C1825	长指形Long finger shape	0	HR	0
H18-GPJ	长牛角形Long bovine horn shape	6.67	R	22.00
XXYSJ-1	扁灯笼形Flat lantern shape	0	HR	0
小米辣	短指形Short finger shape	0	HR	0
H19-STJ-8	长锥形Long conical shape	4.00	R	15.00
H19-SZL-75	短羊角形Short caprine horn shape	19.99	MR	46.00
V06C0954	短羊角形Short caprine horn shape	2.00	R	10.00
佘干椒	短羊角形Short caprine horn shape	5.00	R	25.00
V06C0132	短羊角形Short caprine horn shape	1.18	R	6.00
CF-4	长羊角形Long caprine horn shape	1.11	R	6.00
H19-STJ-74	短指形Short finger shape	0	HR	0
H17-XM39-01	长锥形Long conical shape	3.53	R	18.00
H18-PC-YS-02	长羊角形Long caprine horn shape	1.43	R	7.00
V06C0670	长锥形Long conical shape	4.00	R	15.00
V06C1220	短羊角形Short caprine horn shape	4.00	R	20.00
8701	短牛角形Short bovine horn shape	10.00	R	25.00
V06C0503	长羊角形Long caprine horn shape	4.00	R	5.00
H19-1021	长牛角形Long bovine horn shape	10.00	R	15.00
H19-STJ-1	短牛角形Short bovine horn shape	14.00	MR	25.00
DS701	圆灯笼形Round lantern shape	1.00	HR	5.00
V06C0573	短羊角形Short caprine horn shape	18.00	MR	45.00
V06C172	短羊角形Short caprine horn shape	0	HR	0
AHYYH005394	短指形Short finger shape	6.00	R	15.00
H11-003	长灯笼形Long lantern shape	2.00	R	5.00
H12-115	长羊角形Long caprine horn shape	15.00	MR	35.00
H14-45	长指形Long finger shape	0	HR	0
AHYYH004309	长羊角形Long caprine horn shape	12.00	MR	30.00
SZHHFB01	线形Line shape	0	HR	0
H15-066	长羊角形Long caprine horn shape	0	HR	0
潜山辣椒	短羊角形Short caprine horn shape	6.00	R	15.00
H14-070	短羊角形Short caprine horn shape	3.00	R	10.00
AHYYH004115	长指形Long finger shape	4.00	R	10.00
H19-STJ-76	短羊角形Short caprine horn shape	17.00	MR	35.00
H11-011	短牛角形Short bovine horn shape	6.00	R	15.00
H19-STJ-10	短羊角形Short caprine horn shape	18.00	MR	25.00
V06C1902	长羊角形Long caprine horn shape	6.15	R	23.00
V06C1704	短羊角形Short caprine horn shape	4.00	R	15.00
H19-SZL-25	长牛角形Long bovine horn shape	11.00	MR	20.00
H19-LSF	长羊角形Long caprine horn shape	18.67	MR	40.00
M19-SZL-41	线形Line shape	7.00	R	25.00
AHYYH005152	短指形Short finger shape	3.16	R	11.00
Y-417	线形Line shape	7.37	R	16.00
2016-56	长灯笼形Long lantern shape	16.67	MR	25.00
V06C1657	长灯笼形Long lantern shape	6.15	R	31.00
H18-56-3	线形Line shape	14.29	MR	38.00
H18×9931 BS1	短羊角形Short caprine horn shape	4.00	R	5.00
V06C1205	短羊角形Short caprine horn shape	16.92	MR	31.00
H19-STJ-3	长指形Long finger shape	3.00	R	15.00
H19-SZL-70	长指形Long finger shape	5.00	R	15.00
V06C0330	长羊角形Long caprine horn shape	10.43	MR	22.00
V06V0960	长指形Long finger shape	0	HR	0
AHYYH005208	长羊角形Long caprine horn shape	6.00	R	15.00
AHYYH004364	短羊角形Short caprine horn shape	2.00	R	10.00
H19-STJ-7	短指形Short finger shape	1.00	HR	5.00
AHYYH005174	长指形Long finger shape	2.86	R	7.00

通路 Pathway	基因ID Gene ID	log₂FC	基因特征 Genetic trait
MAPK信号通路，植物—病原互作，激素信号转导 MAPK signaling pathway，plant pathogen interaction，plant hormone signaling transduction	NewGene_12193	5.13	BAK1
	Can07G034320	2.01	BAK1
	Can01G023360	6.06	BAK1
	Can08G028150	9.78	PR1
MAPK信号通路，植物—病原互作 MAPK signaling pathway，plant pathogen interaction	Can07G002780	7.42	FLS2
	Can04G014490	3.36	WRKY33
	NewGene_13172	1.75	FLS2
	Can04G029410	1.46	FLS2
植物—病原互作 Plant pathogen interaction	Can09G002790	2.65	RPS2
	Can10G030550	12.22	RPS2
	Can07G025330	2.91	PBS1
	NewGene_11156	3.21	RPM1
	Can06G035780	5.60	R1B-23
	Can09G017290	2.79	RIN_4
	Can06G036020	2.49	R1A-4

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辣椒种质资源疫病抗性鉴定及抗病基因挖掘

Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources

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