基于Pull-Down/MS技术筛选柑橘CsMYC2和CsMPK6的互作蛋白

doi:10.16420/j.issn.0513-353x.2023-0744

园艺学报 ›› 2024, Vol. 51 ›› Issue (12): 2913-2927.doi: 10.16420/j.issn.0513-353x.2023-0744

基于Pull-Down/MS技术筛选柑橘CsMYC2和CsMPK6的互作蛋白

蒋政华¹, 刘胜军¹, 刘文洁¹, 魏冉冉¹, 何政辰¹, 程超³, 唐政³, 刘保艳³, 谢宗周¹, 叶俊丽¹, 柴利军¹^,², 岳鹏涛¹^,^*(), 邓秀新¹^,²^,^*()

¹华中农业大学园艺林学学院，果蔬园艺作物种质创新与利用全国重点实验室，武汉 430070
²湖北洪山实验室，武汉 430070
³长江勘测规划设计研究有限责任公司，武汉 430010

收稿日期:2024-05-24 修回日期:2024-09-12 出版日期:2024-12-25 发布日期:2024-12-13
通讯作者:
^* E-mail：xxdeng@mail.hzau.edu.cn，
E-mail：louisaimeyue@126.com
基金资助:
中国博士后科学基金特别资助项目(2022T150248); 国家自然科学基金重点项目(31930095); 国家重点研发计划项目(2021YFD1200202); 长江勘测规划设计研究有限责任公司自主创新项目(CX2021Z52)

Screening and Identification of Interacting Proteins of CsMYC2 and CsMPK6 in Citrus Based on Pull-Down/MS Technique

JIANG Zhenghua¹, LIU Shengjun¹, LIU Wenjie¹, WEI Ranran¹, HE Zhengchen¹, CHENG Chao³, TANG Zheng³, LIU Baoyan³, XIE Zongzhou¹, YE Junli¹, CHAI Lijun¹^,², YUE Pengtao¹(), DENG Xiuxin¹^,²()

¹National Key Laboratory of Germplasm Innovation & Utilization of Horticultural Crops，Department of Horticulture and Forestry，Huazhong Agricultural University，Wuhan 430070，China
²Hubei Hongshan Laboratory，Wuhan 430070，China
³Changjiang Survey，Planning Design and Research Co.，Ltd，Wuhan 430010，China

Received:2024-05-24 Revised:2024-09-12 Published:2024-12-25 Online:2024-12-13

摘要/Abstract

摘要：

为探究CsMPK6-CsMYC2模块的互作网络，通过与CsMPK6-CsMYC2模块互作筛选鉴定参与茉莉酸介导的柑橘果皮着色过程的蛋白。利用Pull-Down联合质谱技术，在茉莉酸甲酯（MeJA）处理的柑橘果皮总蛋白中筛选与CsMPK6-CsMYC2模块互作的蛋白。对互作蛋白进行生信分析，发现相关蛋白参与次生代谢物的生物合成、植物丝裂原活化蛋白激酶信号通路、激素响应等生物学过程。其中，Trihelix类转录因子CsGT2和类萌发素蛋白CsGLP对果实发育有重要作用，进一步确认CsGT2与CsMYC2、CsGLP与CsMPK6以及CsGLP与CsMYC2的互作。分析CsGT2和CsGLP启动子的顺式作用元件，发现CsGT2和CsGLP的启动子上含有多个光、激素及逆境响应相关元件。本研究为难以通过酵母双杂交系统或IP-MS技术筛选互作蛋白的物种提供方法参考，同时也为深入研究茉莉酸介导的柑橘果实着色调控网络提供了基因储备。

关键词: 柑橘, 着色, 茉莉酸, Pull-Down/MS, 互作蛋白

Abstract:

The purpose of this study is to explore the interaction network of CsMPK6-CsMYC2 module and identify the proteins involved in the citrus peel coloring process mediated by jasmonic acid through the interaction screening with CsMPK6-CsMYC2 module. To this purpose，Pull-Down with mass spectrometry technique（Pull-Down/MS）was performed to screen and identify the CsMPK6-CsMYC2 interacting proteins using the total proteins isolated from MeJA-treated citrus fruit. The interactive proteins were involved in secondary metabolites biosynthesis，plant mitogen-activated protein kinase signaling pathway，ribosome pathway，stress response，hormone response，abiotic stress responses and other biological processes according to bioinformatics analysis. Among them，trihelix transcription factor CsGT2 and germin-like protein CsGLP play important roles in fruit development. Therefore，the interactions of CsGT2 with CsMYC2，CsGLP with CsMPK6 and CsGLP with CsMYC2 were further confirmed. The promoters of CsGT2 and CsGLP contained multiple cis-acting elements involving in light，hormone and stress-response. This work provides a reference for species that are difficult to screen interacting proteins by using yeast two-hybrid system and IP-MS technology. Meanwhile，this work provides gene pool for further study regarding the regulatory network for the jasmonate-induced coloration in citrus fruit.

Key words: citrus, coloration, jasmonates, Pull-Down/MS, protein interaction

蒋政华, 刘胜军, 刘文洁, 魏冉冉, 何政辰, 程超, 唐政, 刘保艳, 谢宗周, 叶俊丽, 柴利军, 岳鹏涛, 邓秀新. 基于Pull-Down/MS技术筛选柑橘CsMYC2和CsMPK6的互作蛋白[J]. 园艺学报, 2024, 51(12): 2913-2927.

JIANG Zhenghua, LIU Shengjun, LIU Wenjie, WEI Ranran, HE Zhengchen, CHENG Chao, TANG Zheng, LIU Baoyan, XIE Zongzhou, YE Junli, CHAI Lijun, YUE Pengtao, DENG Xiuxin. Screening and Identification of Interacting Proteins of CsMYC2 and CsMPK6 in Citrus Based on Pull-Down/MS Technique[J]. Acta Horticulturae Sinica, 2024, 51(12): 2913-2927.

图/表 11

参考文献 46

[1]	Bruno L, Spadafora N D, Iaria D, Chiappetta A, Bitonti M B. 2014. Developmental stimuli and stress factors affect expression of ClGLP1,an emerging allergen-related gene in Citrus limon. Plant Physiology and Biochemistry,79:31-40.
[2]	Benatti P, Basile V, Merico D, Fantoni L I, Tagliafico E, Imbriano C. 2008. A balance between NF-Y and p 53 governs the pro- and anti-apoptotic transcriptional response. Nucleic Acids Research,36:1415-1428.
[3]	Chen Xiuzhen. 2020. Study on the regulatory mechanism of PatPTS by transcription factors in patchouli alcohol biosynthetic pathway[Ph. D. Dissertation]. Guangzhou: Guangzhou University of Chinese Medicine. (in Chinese)
	陈秀珍. 2020. 广藿香醇生物合成途径中转录因子对PatPTS的调控机制研究[博士论文]. 广州: 广州中医药大学.
[4]	Cui Baolu, Chen Zhongping, Sun Tingting, Long Junlin, Wang Xianxia, Zeng Sijin, Shi Yiran. 2023. Expressions and responses to abiotic stresses and plant growth regulator of tomato GT-1. Fujian Journal of Agricultural Sciences, 38 (2):144-150. (in Chinese)
	崔宝禄, 陈忠平, 孙婷婷, 龙俊霖, 王先霞, 曾思进, 石怡然. 2023. 番茄GT-1基因组织表达模式及非生物胁迫、植物生长调节剂响应分析. 福建农业学报, 38 (2):144-150.
[5]	El-Sharkawy I, Mila I, Bouzayen M, Jayasankar S. 2010. Regulation of two germin-like protein genes during plum fruit development. Journal of Experimental Botany, 61 (6):1761-1770. doi: 10.1093/jxb/erq043 pmid: 20202999
[6]	Green P J, Yong M H, Cuozzo M, Kano-Murakami Y, Silverstein P, Chua N H. 1988. Binding site requirements for pea nuclear protein factor GT-1 correlate with sequences required for light-dependent transcriptional activation of the rbcS-3A gene. The EMBO Journal, 7 (13):4035-4044.
[7]	Ilyas M, Ali I, Nasser Binjawhar D, Ullah S, Eldin S M, Ali B, Iqbal R, Bokhari S H A, Mahmood T. 2023. Molecular characterization of germin-like protein genes in Zea mays(ZmGLPs)using various in silico approaches. ACS Omega, 8 (18):16327-16344.
[8]	Jin Rong, Liu ming, Zhao Peng, Zhang Qiangqiang, Zhang Aijun, Tang Zhonghou. 2021. IbMKP6,A mitogen-activated protein kinase,confers low temperature tolerance in sweetpotato. Scientia Agricultura Sinica, 54 (20):4265-4273. (in Chinese) doi: 10.3864/j.issn.0578-1752.2021.20.002
	靳容, 刘明, 赵鹏, 张强强, 张爱君, 唐忠厚. 2021. 甘薯丝裂原活化蛋白激酶MPK6对低温胁迫的响应. 中国农业科学, 54 (20):4265-4273. doi: 10.3864/j.issn.0578-1752.2021.20.002
[9]	Lalonde S, Ehrhardt D W, Loqué D, Chen J, Rhee S Y, Frommer W B. 2008. Molecular and cellular approaches for the detection of protein-protein interactions:latest techniques and current limitations. The Plant Journal, 53 (4):610-635. doi: 10.1111/j.1365-313X.2007.03332.x pmid: 18269572
[10]	Li Bo, Xu Xinru. 2021. Research progress on plant germin-like proteins. Modern Agricultural Science and Technology,(19):199-201,204. (in Chinese)
	李波, 许昕薷. 2021. 植物类萌发素蛋白研究进展. 现代农业科技,(19):199-201,204.
[11]	Li C J, Shi L, Wang Y N, Li W, Chen B Q, Zhu L, Fu Y. 2020a. Arabidopsis ECAP is a new adaptor protein that connects JAZ repressors with the TPR2 co-repressor to suppress jasmonate-responsive anthocyanin accumulation. Molecular Plant, 13 (2):246-265.
[12]	Li M, Yu G, Cao C, Liu P. 2021. Metabolism, signaling and transport of jasmonates. Plant Communication, 2 (5):100231.
[13]	Li X, Liu C, Zhao Z, Ma D, Zhang J, Yang Y, Liu Y, Liu H. 2020b. COR27 and COR28 are novel regulators of the COP1-HY 5 regulatory hub and photomorphogenesis in Arabidopsis. The Plant Cell, 32 (10):3139-3154.
[14]	Liu Fan, Tian Na, Sun Xueli, Liu Jiapeng, Wu Junwei, Huang Yuji, Cheng Chunzhen. 2020. Genome-wide identification and expression analysis of banana GLP gene family. Acta Horticulturae Sinica, 47 (10):1930-1946. (in Chinese) doi: 10.16420/j.issn.0513-353x.2019-0983 URL
	刘范, 田娜, 孙雪丽, 刘嘉鹏, 伍俊为, 黄玉吉, 程春振. 2020. 香蕉GLP基因家族全基因组鉴定及表达分析. 园艺学报, 47 (10):1930-1946.
[15]	Liu H M Z, Wang X, Liu S J, Huang Y, Guo Y X, Xie W Z, Liu H, Muhammad T Q, Xu Q, Chen L L. 2022. Citrus pan-genome to breeding database(CPBD):a comprehensive genome database for citrus breeding. Molecular Plant, 15 (10):1503-1505.
[16]	Liu L, Wei J, Zhang M, Zhang L, Li C, Wang Q. 2012. Ethylene independent induction of lycopene biosynthesis in tomato fruits by jasmonates. Journal of Experimental Botany, 63 (16):5751-5761. doi: 10.1093/jxb/ers224 pmid: 22945939
[17]	Liu Zimeng. 2022. A preliminary study on the regulation of transcription factor SlGT 30 on the coloration and ripening process of tomato fruit[M. S. Dissertation]. Hangzhou:Zhejiang University. (in Chinese)
	刘子萌. 2022. 转录因子SlGT30对番茄果实着色和成熟进程的调控初探[硕士论文]. 杭州: 浙江大学.
[18]	Méteignier L V. 2023. Tanned but not burned:a negative feedback loop controls Citrus fruit coloration. The Plant Cell, 35 (4):1163-1164.
[19]	Nagano Y. 2000. Several features of the GT-factor trihelix domain resemble those of the Myb DNA-binding domain. Plant Physiology,124:491-494.
[20]	Nisar N, Li L, Lu S, Khin N C, Pogson B J. 2015. Carotenoid metabolism in plants. Molecular Plant, 8 (1):68-82. doi: 10.1016/j.molp.2014.12.007 pmid: 25578273
[21]	Rodrigo M J, Alquezar B, Alos E, Medina V, Carmona L, Bruno M, Al-Babili S, Zacarias L. 2013. A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments. Journal of Experimental Botany,64:4461-4478.
[22]	Ruiz-Sola M A, Rodriguez-Concepcion M. 2012. Carotenoid biosynthesis in Arabidopsis:a colorful pathway. Arabidopsis Book,10:e0158.
[23]	Smalle J, Kurepa J, Haegman M, Gielen J, van Montagu M, van der Straeten D. 1998. The trihelix DNA-binding motif in higher plants is not restricted to the transcription factors GT-1 and GT-2. Proceedings of the National Academy of Sciences of the United States of America,95: 3318-3322.
[24]	Song Lulu. 2017. Functional characterization and regulation mechanism of ShGT-1 gene in stress-responsiveness in tomato[M. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	宋露露. 2017. 番茄逆境响应基因ShGT-1的功能鉴定及调控机制解析[硕士论文]. 武汉: 华中农业大学.
[25]	Sun B, Li W, Ma Y, Yu T, Huang W, Ding J, Yu H, Jiang L, Zhang J, Lü S, Yang J, Yan S, Liu B, Liu Q. 2023. OsGLP3-7 positively regulates rice immune response by activating hydrogen peroxide,jasmonic acid,and phytoalexin metabolic pathways. Molecular Plant Pathology, 24 (3):248-261.
[26]	Sun Jiamao, Cui Quanshi, Wang Yuqing, Si Yajing, Shi Yufan, Bu Haidong, Yuan Hui, Wang Aide. 2022. Effects of brassinolides and methyl jasmonate spraying on the postharvest quality of apple fruit. Acta Horticulturae Sinica, 49 (10):2236-2248. (in Chinese) doi: 10.16420/j.issn.0513-353x.2022-0594 URL
	孙嘉茂, 崔全石, 王语晴, 司雅静, 时瑀繁, 卜海东, 袁晖, 王爱德. 2022. 苹果采前喷施EBR与MeJA对采后品质的影响. 园艺学报, 49 (10):2236-2248. doi: 10.16420/j.issn.0513-353x.2022-0594 URL
[27]	Sun Ruixue, Mi Wei, Ye Zihong. 2022. Research advances in protein interactions based on mass spectrometry. Current Biotechnology, 12 (2):161-167. (in Chinese) doi: 10.19586/j.2095-2341.2021.0137
	孙瑞雪, 米薇, 叶子弘. 2022. 基于质谱技术的蛋白质互作研究进展. 生物技术进展, 12 (2):161-167. doi: 10.19586/j.2095-2341.2021.0137
[28]	Takahashi F, Yoshida R, Ichimura K, Mizoguchi T, Seo S, Yonezawa M, Maruyama K, Yamaguchi-Shinozaki K, Shinozaki K. 2007. The mitogen-activated protein kinase cascade MKK3-MPK 6 is an important part of the jasmonate signal transduction pathway in Arabidopsis. The Plant Cell, 19 (3):805-818.
[29]	Tian T, Liu Y, Yan H Y, You Q, Yi X, Du Z, Xu W Y, Su Z. 2017. agriGO v2.0:a GO analysis toolkit for the agricultural community. Nucleic Acids Research, 45 (W1):W122-W129.
[30]	Tsuneaki A, Guillaume T, Joulia P, Matthew R W, Wan-Ling C, Lourdes G G, Thomas B, Frederick M A, Jen S. 2002. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature, 415 (6875):977-983.
[31]	Turner J G, Ellis C, Devoto A. 2002. The jasmonate signal pathway. The Plant Cell,14 (Supplement):153-164.
[32]	Tzortzakis N G, Economakis C D. 2007. Maintaining postharvest quality of the tomato fruit by employing methyl jasmonate and ethanol vapor treatment. Journal of Food Quality, 30 (5):567-580.
[33]	Ulmasov T, Hagen G, Guilfoyle T J. 1999. Activation and repression of transcription by auxin-response factors. Proceedings of the National Academy of Sciences of the United States of America, 96 (10):5844-5849.
[34]	Wang B, Luo Q, Li Y, Du K, Wu Z, Li T, Shen W H, Huang C H, Gan J, Dong A. 2022. Structural insights into partner selection for MYB and bHLH transcription factor complexes. Nature Plants, 8 (9):1108-1117. doi: 10.1038/s41477-022-01223-w pmid: 35995835
[35]	Wang Z, Liu Q, Wang H, Zhang H, Xu X, Li C, Yang C. 2016. Comprehensive analysis of trihelix genes and their expression under biotic and abiotic stresses in Populus trichocarpa. Scientific Reports,6:36274.
[36]	Wu Tong. 2022. Phylogenetic analysis of Trihelix transcription factor family in plants[Ph. D. Dissertation]. Tangshan: North China University of Science and Technology. (in Chinese)
	吴彤. 2022. 植物中三螺旋转录因子家族的系统进化分析[博士论文]. 唐山: 华北理工大学.
[37]	Xie Z M, Zou H F, Lei G, Wei W, Zhou Q Y, Niu C F, Liao Y, Tian A G, Ma B, Zhang W K, Zhang J S, Chen S Y. 2009. Soybean Trihelix transcription factors GmGT-2A and GmGT-2B improve plant tolerance to abiotic stresses in transgenic Arabidopsis. PLoS ONE,4:e6898.
[38]	Xu Jianfeng. 2003. Studies on effect of methyl jasmonate on colour development of apples[M. D. Dissertation]. Baoding: Henan Agricultural University. (in Chinese)
	许建锋. 2003. 茉莉酸甲酯对苹果色泽发育效应的研究[硕士论文]. 保定: 河南农业大学
[39]	Yang Y, Yang X H, Guo X, Hu X X, Dong D F, Li G C, Xiong X Y. 2022. Exogenously applied methyl Jasmonate induces early defense related genes in response to Phytophthora infestans infection in potato plants. Horticultural Plant Journal, 8 (4):511-526.
[40]	Yue P, Jiang Z, Sun Q, Wei R, Yin Y, Xie Z, Larkin R M, Ye J, Chai L, Deng X. 2023. Jasmonate activates a CsMPK6-CsMYC 2 module that regulates the expression of beta-citraurin biosynthetic genes and fruit coloration in orange(Citrus sinensis). The Plant Cell, 35 (4):1167-1185.
[41]	Yuan H, Zhang J, Nageswaran D, Li L. 2015. Carotenoid metabolism and regulation in horticultural crops. Horticulture Research, 2 (1):15036.
[42]	Zeng Yunliu. 2014. Investigation on chromoplast structural characteristics and their differentiation in citrus flesh during fruit ripening [Ph. D. Dissertation]. Wuhan:Huazhong Agricultural University. (in Chinese)
	曾云流. 2014. 柑橘果肉有色体的结构特征及其分化研究[博士论文]. 武汉:华中农业大学.
[43]	Zhai Q, Yan L, Tan D, Chen R, Sun J, Gao L, Dong M Q, Wang Y, Li C. 2013. Phosphorylation-coupled proteolysis of the transcription factor MYC 2 is important for jasmonate-signaled plant immunity. PLoS Genetics, 9 (4):e1003422.
[44]	Zheng Jiarui, Yang Xiaoyan, Ye Jiabao, Liao Yongling, Xu Feng. 2023. Advances in the functional studies of MYC 2 transcription factor in plants. Acta Horticulturae Sinica, 50 (4):896-908. (in Chinese) doi: 10.16420/j.issn.0513-353x.2022-0102 URL
	郑嘉瑞, 杨晓燕, 叶家保, 廖咏玲, 许锋. 2023. MYC2转录因子在植物中的功能研究进展. 园艺学报, 50 (4):896-908. doi: 10.16420/j.issn.0513-353x.2022-0102 URL
[45]	Zheng X, Liu H, Ji H, Wang Y, Dong B, Qiao Y, Liu M, Li X. 2016. The wheat GT factor TaGT2L1D negatively regulates drought tolerance and plant development. Scientific Reports,6:27042.
[46]	Zhu J Y, Chen H R, Liu L, Xia X B, Yan X M, Mi X Z, Liu S R, Wei C L. 2024. JA-mediated MYC2/LOX/AOS feedback loop regulates osmotic stress response in tea plant. Horticultural Plant Journal, 10 (3):931-946.

用途	引物名称	引物序列（5′-3′）
Purpose	Primer name	Primer sequence
全长扩增	CsGLP-F	ATGAAGGTAATTCAATTTCTTATAGGTTTT
Full length amplification	CsGLP-R	CTAATTGCCATTAATGAACTTATTTTGAAG
	CsGT2-F	ATGGAAGGCCAATCATCACTAACT
	CsGT2-R	CTAAACTGTTAAATCGTAATCAGCCC
蛋白纯化	CsGLP-C2X-EcoRⅠ-F	GAGGGAAGGATTTCA GAATTCATGAAGGTAATTCAATTTCTTAT
Protein purification	CsGLP-C2X-SalⅠ-R	CAAGCTTGCCTGCAG GTCGACCTAATTGCCATTAATGAACTTATTT
	CsGT2-GST-EcoRⅠ-F	CCGCGTGGATCCCCG GAATTCATGGAAGGCCAATCATCACTAACT
	CsGT2-GST-SalⅠ-R	GATGCGGCCGCTCGA GTCGACCTAAACTGTTAAATCGTAATCAGCCC
荧光素酶互补实验	CsGT2-772-F	TACGCGTCCCGGGGC GGTACCATGGAAGGCCAATCATCACTAACT
Luciferase complementation	CsGT2-772-R	ACGAAAGCTCTGCAG GTCGACCTAAACTGTTAAATCGTAATCAGCCC
assay	CsGLP-771-F	ACGGGGGACGAGCTC GGTACCATGAAGGTAATTCAATTTCTTATAGGTTT
	CsGLP-771-R	CGCGTACGAGATCTG GTCGACCTAATTGCCATTAATGAACTTATTTTGAA

用途	引物名称	引物序列（5′-3′）
Purpose	Primer name	Primer sequence
全长扩增	CsGLP-F	ATGAAGGTAATTCAATTTCTTATAGGTTTT
Full length amplification	CsGLP-R	CTAATTGCCATTAATGAACTTATTTTGAAG
	CsGT2-F	ATGGAAGGCCAATCATCACTAACT
	CsGT2-R	CTAAACTGTTAAATCGTAATCAGCCC
蛋白纯化	CsGLP-C2X-EcoRⅠ-F	GAGGGAAGGATTTCA GAATTCATGAAGGTAATTCAATTTCTTAT
Protein purification	CsGLP-C2X-SalⅠ-R	CAAGCTTGCCTGCAG GTCGACCTAATTGCCATTAATGAACTTATTT
	CsGT2-GST-EcoRⅠ-F	CCGCGTGGATCCCCG GAATTCATGGAAGGCCAATCATCACTAACT
	CsGT2-GST-SalⅠ-R	GATGCGGCCGCTCGA GTCGACCTAAACTGTTAAATCGTAATCAGCCC
荧光素酶互补实验	CsGT2-772-F	TACGCGTCCCGGGGC GGTACCATGGAAGGCCAATCATCACTAACT
Luciferase complementation	CsGT2-772-R	ACGAAAGCTCTGCAG GTCGACCTAAACTGTTAAATCGTAATCAGCCC
assay	CsGLP-771-F	ACGGGGGACGAGCTC GGTACCATGAAGGTAATTCAATTTCTTATAGGTTT
	CsGLP-771-R	CGCGTACGAGATCTG GTCGACCTAATTGCCATTAATGAACTTATTTTGAA

基因ID	注释	基因ID	注释
Gene ID	Annotation	Gene ID	Annotation
CISIN 1g039131mg	MYB-DNA结合结构域蛋白MYB-DNA domain-containing protein（CsGT2）	CISIN 1g027381mg	类萌发素蛋白 Germin-like protein（CsGLP）
CISIN 1g001709mg	含有Lipase 3结构域的蛋白 Lipase 3 domain-containing protein	CISIN 1g031118mg	含VOC结构域的蛋白 VOC domain-containing protein
CISIN 1g028399mg	LEA 2结构域蛋白 LEA_2 domain-containing protein	CISIN 1g011329mg	液泡质子泵亚基B Vacuolar proton pump subunit B
CISIN 1g013829mg	抗坏血酸过氧化酶 L-ascorbate peroxidase	CISIN 1g031168mg	含有Usp结构域的蛋白质 Usp domain-containing protein
Annotation	Gene ID	Annotation
CISIN 1g026316mg	KH 2型结构域蛋白 KH type-2 domain-containing protein	CISIN 1g005713mg	含有Tr型G结构域的蛋白 Tr-type G domain-containing protein
CISIN 1g015012mg	异柠檬酸脱氢酶 Isocitrate dehydrogenase[NADP]	CISIN 1g003492mg	蔗糖合酶 Sucrose synthase
CISIN 1g017413mg	异柠檬酸脱氢酶（NAD）亚基，线粒体 Isocitrate dehydrogenase[NAD]subunit，mitochondrial	CISIN 1g031218mg	含SHSP结构域的蛋白 SHSP domain-containing protein
CISIN 1g032645mg	组蛋白 H3 Histone H3	CISIN 1g015873mg	S-腺苷甲硫氨酸合酶 S-Adenosylmethionine synthase
CISIN 1g013352mg	鸟苷核苷酸二磷酸解离抑制剂 Guanosine nucleotide diphosphate dissociation inhibitor	CISIN 1g012947mg	Sacchrp dh NADP结构域蛋白 Sacchrp dh NADP domain-containing protein
CISIN 1g024728mg	含有GRAM结构域的蛋白 GRAM domain-containing protein	CISIN 1g012719mg	RuvB-like解旋酶 RuvB-like helicase
CISIN 1g027134mg	谷胱甘肽过氧化物酶 Glutathione peroxidase	CISIN 1g007404mg	RNA聚合酶因子 RNA polymerase sigma factor
CISIN 1g028808mg	谷氧还蛋白依赖过氧化物还原酶 Glutaredoxin-dependent peroxiredoxin	CISIN 1g031854mg	含有核糖体s13 n结构域的蛋白 Ribosomal S13 N domain-containing protein
CISIN 1g020203mg	LRRNT 2结构域蛋白 LRRNT 2 domain-containing protein	CISIN 1g036921mg	丙酮酸激酶 Pyruvate kinase
CISIN 1g018326mg	果糖二磷酸醛缩酶 Fructose-bisphosphate aldolase	CISIN 1g026587mg	蛋白酶体亚基 Proteasome subunit beta
CISIN 1g023956mg	铁蛋白 Ferritin	CISIN 1g023059mg	抑制素蛋白 Prohibitin
CISIN 1g027898mg	内质网跨膜蛋白Endoplasmic reticulum transmembrane protein	CISIN 1g027094mg	PMEI结构域蛋白 PMEI domain-containing protein
CISIN 1g027254mg	含EF1 GNE结构域的蛋白 EF1 GNE domain-containing protein	CISIN 1g0180941mg	PKS ER结构域蛋白 PKS ER domain-containing protein
CISIN 1g0075121mg	D-3-磷酸甘油酸脱氢酶 D-3-phosphoglycerate dehydrogenase	CISIN 1g014812mg	Pinin SDK memA结构域蛋白 Pinin SDK memA domain-containing protein
CISIN 1g023850mg	细胞色素b-c1复合体亚基Rieske，线粒体Cytochrome b-c1 complex subunit Rieske，mitochondrial	CISIN 1g043137mg	磷酸丙酮酸水合酶 Phosphopyruvate hydratase
CISIN 1g002012mg	含有Clp R结构域的蛋白 Clp R domain-containing protein	CISIN 1g004579mg	磷脂酶D Phospholipase D
CISIN 1g044386mg	过氧化氢酶 Catalase	CISIN 1g011942mg	磷酸甘油酸酯激酶Phosphoglycerate kinase
CISIN 1g023810mg	C2结构域蛋白 C2 domain-containing protein	CISIN 1g0064861mg	磷酸烯醇丙酮酸羧激酶（ATP） Phosphoenolpyruvate carboxykinase（ATP）
CISIN 1g029006mg	ATP-synt DE N结构域蛋白 ATP-synt DE N domain-containing protein	CISIN 1g000370mg	PHD型结构域蛋白 PHD-type domain-containing protein
CISIN 1g008610mg	ATP合成酶亚基 ATP synthase subunit beta	CISIN 1g038305mg	含PHB型结构域蛋白 PHB domain-containing protein
CISIN 1g035973mg	膜联蛋白 Annexin	CISIN 1g018811mg	过氧化物酶 Peroxidase
CISIN 1g012095mg	Aminotran 1 2结构域蛋白 Aminotran 1 2 domain-containing protein	CISIN 1g011566mg	含有肽酶A1结构域的蛋白 Peptidase A1 domain-containing protein
CISIN 1g018374mg	Aldo ket red结构域蛋白 Aldo ket red domain-containing protein	CISIN 1g014555mg	PCI结构域蛋白 PCI domain-containing protein
CISIN 1g016996mg	ADP/ATP移位酶 ADP/ATP translocase	CISIN 1g022196mg	PAP 原纤维蛋白结构域蛋白 PAP fibrillin domain-containing protein
CISIN 1g011464mg	腺苷高半胱氨酸酶 Adenosyl homocysteinase	CISIN 1g026906mg	核苷二磷酸激酶 Nucleoside diphosphate kinase
CISIN 1g008996mg	丙酮酸脱氢酶复合物的乙酰转移酶组分 Acetyltransferase component of pyruvate dehydrogenase complex	CISIN 1g033151mg	非特异性脂质转移蛋白 Non-specific lipid-transfer protein
CISIN 1g038101mg	AAA结构域蛋白 AAA domain-containing protein	CISIN 1g020296mg	NADH-细胞色素b5还原酶 NADH-cytochrome b5 reductase
CISIN 1g025016mg	60S核糖体蛋白L7a 60S ribosomal protein L7a	CISIN 1g031073mg	V型质子ATP酶蛋白脂亚基 V-type proton ATPase proteolipid subunit
CISIN 1g015722mg	(S)-2-羟酸氧化酶 (S)-2-hydroxy-acid oxidase	CISIN 1g026558mg	Methyl transfer 11结构域蛋白 Methyltransf 11 domain-containing protein
CISIN 1g018314mg	苹果酸脱氢酶 Malate dehydrogenase	CISIN 1g017293mg	Methylthioribose-1-磷酸异构酶 Methylthioribose-1-phosphate isomerase

基因ID	注释	基因ID	注释
Gene ID	Annotation	Gene ID	Annotation
CISIN 1g039131mg	MYB-DNA结合结构域蛋白MYB-DNA domain-containing protein（CsGT2）	CISIN 1g027381mg	类萌发素蛋白 Germin-like protein（CsGLP）
CISIN 1g001709mg	含有Lipase 3结构域的蛋白 Lipase 3 domain-containing protein	CISIN 1g031118mg	含VOC结构域的蛋白 VOC domain-containing protein
CISIN 1g028399mg	LEA 2结构域蛋白 LEA_2 domain-containing protein	CISIN 1g011329mg	液泡质子泵亚基B Vacuolar proton pump subunit B
CISIN 1g013829mg	抗坏血酸过氧化酶 L-ascorbate peroxidase	CISIN 1g031168mg	含有Usp结构域的蛋白质 Usp domain-containing protein
Annotation	Gene ID	Annotation
CISIN 1g026316mg	KH 2型结构域蛋白 KH type-2 domain-containing protein	CISIN 1g005713mg	含有Tr型G结构域的蛋白 Tr-type G domain-containing protein
CISIN 1g015012mg	异柠檬酸脱氢酶 Isocitrate dehydrogenase[NADP]	CISIN 1g003492mg	蔗糖合酶 Sucrose synthase
CISIN 1g017413mg	异柠檬酸脱氢酶（NAD）亚基，线粒体 Isocitrate dehydrogenase[NAD]subunit，mitochondrial	CISIN 1g031218mg	含SHSP结构域的蛋白 SHSP domain-containing protein
CISIN 1g032645mg	组蛋白 H3 Histone H3	CISIN 1g015873mg	S-腺苷甲硫氨酸合酶 S-Adenosylmethionine synthase
CISIN 1g013352mg	鸟苷核苷酸二磷酸解离抑制剂 Guanosine nucleotide diphosphate dissociation inhibitor	CISIN 1g012947mg	Sacchrp dh NADP结构域蛋白 Sacchrp dh NADP domain-containing protein
CISIN 1g024728mg	含有GRAM结构域的蛋白 GRAM domain-containing protein	CISIN 1g012719mg	RuvB-like解旋酶 RuvB-like helicase
CISIN 1g027134mg	谷胱甘肽过氧化物酶 Glutathione peroxidase	CISIN 1g007404mg	RNA聚合酶因子 RNA polymerase sigma factor
CISIN 1g028808mg	谷氧还蛋白依赖过氧化物还原酶 Glutaredoxin-dependent peroxiredoxin	CISIN 1g031854mg	含有核糖体s13 n结构域的蛋白 Ribosomal S13 N domain-containing protein
CISIN 1g020203mg	LRRNT 2结构域蛋白 LRRNT 2 domain-containing protein	CISIN 1g036921mg	丙酮酸激酶 Pyruvate kinase
CISIN 1g018326mg	果糖二磷酸醛缩酶 Fructose-bisphosphate aldolase	CISIN 1g026587mg	蛋白酶体亚基 Proteasome subunit beta
CISIN 1g023956mg	铁蛋白 Ferritin	CISIN 1g023059mg	抑制素蛋白 Prohibitin
CISIN 1g027898mg	内质网跨膜蛋白Endoplasmic reticulum transmembrane protein	CISIN 1g027094mg	PMEI结构域蛋白 PMEI domain-containing protein
CISIN 1g027254mg	含EF1 GNE结构域的蛋白 EF1 GNE domain-containing protein	CISIN 1g0180941mg	PKS ER结构域蛋白 PKS ER domain-containing protein
CISIN 1g0075121mg	D-3-磷酸甘油酸脱氢酶 D-3-phosphoglycerate dehydrogenase	CISIN 1g014812mg	Pinin SDK memA结构域蛋白 Pinin SDK memA domain-containing protein
CISIN 1g023850mg	细胞色素b-c1复合体亚基Rieske，线粒体Cytochrome b-c1 complex subunit Rieske，mitochondrial	CISIN 1g043137mg	磷酸丙酮酸水合酶 Phosphopyruvate hydratase
CISIN 1g002012mg	含有Clp R结构域的蛋白 Clp R domain-containing protein	CISIN 1g004579mg	磷脂酶D Phospholipase D
CISIN 1g044386mg	过氧化氢酶 Catalase	CISIN 1g011942mg	磷酸甘油酸酯激酶Phosphoglycerate kinase
CISIN 1g023810mg	C2结构域蛋白 C2 domain-containing protein	CISIN 1g0064861mg	磷酸烯醇丙酮酸羧激酶（ATP） Phosphoenolpyruvate carboxykinase（ATP）
CISIN 1g029006mg	ATP-synt DE N结构域蛋白 ATP-synt DE N domain-containing protein	CISIN 1g000370mg	PHD型结构域蛋白 PHD-type domain-containing protein
CISIN 1g008610mg	ATP合成酶亚基 ATP synthase subunit beta	CISIN 1g038305mg	含PHB型结构域蛋白 PHB domain-containing protein
CISIN 1g035973mg	膜联蛋白 Annexin	CISIN 1g018811mg	过氧化物酶 Peroxidase
CISIN 1g012095mg	Aminotran 1 2结构域蛋白 Aminotran 1 2 domain-containing protein	CISIN 1g011566mg	含有肽酶A1结构域的蛋白 Peptidase A1 domain-containing protein
CISIN 1g018374mg	Aldo ket red结构域蛋白 Aldo ket red domain-containing protein	CISIN 1g014555mg	PCI结构域蛋白 PCI domain-containing protein
CISIN 1g016996mg	ADP/ATP移位酶 ADP/ATP translocase	CISIN 1g022196mg	PAP 原纤维蛋白结构域蛋白 PAP fibrillin domain-containing protein
CISIN 1g011464mg	腺苷高半胱氨酸酶 Adenosyl homocysteinase	CISIN 1g026906mg	核苷二磷酸激酶 Nucleoside diphosphate kinase
CISIN 1g008996mg	丙酮酸脱氢酶复合物的乙酰转移酶组分 Acetyltransferase component of pyruvate dehydrogenase complex	CISIN 1g033151mg	非特异性脂质转移蛋白 Non-specific lipid-transfer protein
CISIN 1g038101mg	AAA结构域蛋白 AAA domain-containing protein	CISIN 1g020296mg	NADH-细胞色素b5还原酶 NADH-cytochrome b5 reductase
CISIN 1g025016mg	60S核糖体蛋白L7a 60S ribosomal protein L7a	CISIN 1g031073mg	V型质子ATP酶蛋白脂亚基 V-type proton ATPase proteolipid subunit
CISIN 1g015722mg	(S)-2-羟酸氧化酶 (S)-2-hydroxy-acid oxidase	CISIN 1g026558mg	Methyl transfer 11结构域蛋白 Methyltransf 11 domain-containing protein
CISIN 1g018314mg	苹果酸脱氢酶 Malate dehydrogenase	CISIN 1g017293mg	Methylthioribose-1-磷酸异构酶 Methylthioribose-1-phosphate isomerase

基因ID	注释	基因ID	注释
Gene ID	Annotation	Gene ID	Annotation
CISIN 1g027381mg	类萌发素蛋白 Germin-like protein，CsGLP	CISIN 1g010053mg	bHLH结构域蛋白 BHLH domain-containing protein，CsMYC2
CISIN 1g020203mg	LRRNT 2结构域蛋白 LRRNT 2 domain-containing protein	CISIN 1g013165mg	微管蛋白 Tubulin beta chain
CISIN 1g002055mg	LRRNT 2结构域蛋白 LRRNT 2 domain-containing protein	CISIN 1g002012mg	含Clp R结构域的蛋白 Clp R domain-containing protein
CISIN 1g014614mg	无特征的蛋白质 Uncharacterized protein	CISIN 1g024515mg	核糖体L18e/L15P结构域蛋白 Ribosomal L18e/L15P domain-containing protein
CISIN 1g0132372mg	延长因子1-α Elongation factor 1-alpha	CISIN 1g017081mg	无特征的蛋白质 Uncharacterized protein
CISIN 1g017684mg	无特征的蛋白质 Uncharacterized protein	CISIN 1g006297mg	无特征的蛋白质 Uncharacterized protein
CISIN 1g005713mg	含有Tr型G结构域的蛋白 Tr-type G domain-containing protein	CISIN 1g019445mg	甘油醛-3-磷酸脱氢酶 Glyceraldehyde-3-phosphate dehydrogenase

基于Pull-Down/MS技术筛选柑橘CsMYC2和CsMPK6的互作蛋白

Screening and Identification of Interacting Proteins of CsMYC2 and CsMPK6 in Citrus Based on Pull-Down/MS Technique

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 46

相关文章 15

编辑推荐

Metrics

本文评价

访问总数:　当日访问总数:　当前在线人数:

[1]	杨金磊, 吕壁纹, 陈岳文, 金燕, 杨俊枫, 周铁, 唐俊, 常媛媛, 杨长耀, 卢晓鹏, . 湖南柑橘产区镉污染状况及镉对柑橘发育影响分析[J]. 园艺学报, 2025, 52(1): 200-212.
[2]	董丽婷, 屈荣荣, 庞淑炜, 莫凯琴, 陈爽, 商兰月, 邹修平. 柑橘CsMEKK1-1响应黄龙病菌侵染的表达特征与功能探究[J]. 园艺学报, 2024, 51(9): 2168-2182.
[3]	陈明, 张洁茹, 杨航云, 王印宝, 郑致远, 曾教科, 陈金印, 付永琦, 向妙莲. 茉莉酸甲酯诱导采后脐橙抗青霉病的机制研究[J]. 园艺学报, 2024, 51(9): 2183-2194.
[4]	张文龙, 万润楚, 郑妮, 陈焱, 赖恒鑫, 余歆, 钱春, 曹立. 柑橘不育系新品种‘阳光1号’[J]. 园艺学报, 2024, 51(9): 2221-2222.
[5]	李洁, 武超, 贾祥堑, 王娟. ‘壶瓶枣’果皮着色物质及其相关基因筛选[J]. 园艺学报, 2024, 51(8): 1728-1742.
[6]	杜美霞, 庞淑玮, 董丽婷, 莫凯琴, 候梦圆, 王帅, 邹修平. 柑橘黄龙病菌与寄主互作的分子机制研究进展[J]. 园艺学报, 2024, 51(7): 1623-1638.
[7]	曹惠祥, 罗鑫, 刘婉荣, 管书萍, 王婷婷, 伍小萌, 郭文武, 解凯东. 柑橘2个三倍体果实囊衣发育与化渣性评价[J]. 园艺学报, 2024, 51(6): 1179-1188.
[8]	周慧珍, 张嘉, 胡军华, 李白雪, 曹立, 余歆, 王福生, 邹修平, 周彦. 柑橘感染褐斑病过程中BAG1的作用[J]. 园艺学报, 2024, 51(5): 956-970.
[9]	彭爱红, 张婧芸, 陈志毅, 苏娟, 何永睿, 姚利晓. CsEXPA8过表达对‘晚锦橙’生长及溃疡病抗性的影响[J]. 园艺学报, 2024, 51(5): 971-981.
[10]	游平, 杨进, 周俊, 黄爱军, 鲍敏丽, 易龙. 柑橘黄龙病菌原噬菌体的遗传多样性分析[J]. 园艺学报, 2024, 51(4): 727-736.
[11]	熊志伟, 李智龙, 尹晖, 高玉霞. 柑橘黄龙病菌亚洲种的泛基因组分析[J]. 园艺学报, 2024, 51(4): 737-747.
[12]	易倩, 张曼曼, 汪小利, 冯继鹏, 朱世平, 王福生, 赵晓春. CclSAUR49对柑橘生长及类柠檬苦素代谢的影响[J]. 园艺学报, 2024, 51(3): 479-494.
[13]	孙权, 何政辰, 叶俊丽, 魏冉冉, 尹映紫, 柴利军, 谢宗周, 徐强, 徐娟, 郭文武, 程运江, 邓秀新. 与呼吸跃变型果实共贮藏改善柑橘果实色泽和品质[J]. 园艺学报, 2024, 51(3): 601-615.
[14]	吴卓群, 陈鹏旭, 陈思怡, 张福琼, 方红, 祝建, 谢宗周, 李春龙, 刘继红. 温州蜜柑与椪柑果实日灼发生差异及机制[J]. 园艺学报, 2024, 51(12): 2743-2757.
[15]	姜波, 吕远达, 刘淑梅, 闫化学. 植物激素调控柑橘果实成熟的研究进展[J]. 园艺学报, 2024, 51(12): 2928-2944.

基于Pull-Down/MS技术筛选柑橘CsMYC2和CsMPK6的互作蛋白

Screening and Identification of Interacting Proteins of CsMYC2 and CsMPK6 in Citrus Based on Pull-Down/MS Technique

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 46

相关文章 15

编辑推荐

Metrics

本文评价

访问总数: 当日访问总数: 当前在线人数:

访问总数:　当日访问总数:　当前在线人数: