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园艺学报 ›› 2022, Vol. 49 ›› Issue (7): 1505-1518.doi: 10.16420/j.issn.0513-353x.2021-0474

• 研究论文 • 上一篇    下一篇

百日草花青素苷合成相关MYB转录因子筛选及ZeMYB9功能研究

钱婕妤, 蒋玲莉, 郑钢, 陈佳红, 赖吴浩, 许梦晗, 付建新, 张超()   

  1. 浙江农林大学风景园林与建筑学院,浙江省园林植物种质创新与利用重点实验室,杭州 311300
  • 收稿日期:2022-02-27 修回日期:2022-04-24 出版日期:2022-07-25 发布日期:2022-07-29
  • 基金资助:
    国家级大学生创新创业训练计划项目(202010341022);浙江农林大学大学生科研训练项目(2021KX0069)

Identification and Expression Analysis of MYB Transcription Factors Regulating the Anthocyanin Biosynthesis in Zinnia elegans and Function Research of ZeMYB9

QIAN Jieyu, JIANG Lingli, ZHENG Gang, CHEN Jiahong, LAI Wuhao, XU Menghan, FU Jianxin, ZHANG Chao()   

  1. Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants,School of Landscape Architecture,Zhejiang A & F University,Hangzhou 311300,China
  • Received:2022-02-27 Revised:2022-04-24 Online:2022-07-25 Published:2022-07-29

摘要:

以百日草‘梦境红色’作为试验材料,基于其转录组初步鉴定出33个MYB转录因子。系统进化树分析,鉴定出10个可能参与调控花青素苷合成的R2R3-MYB蛋白,其中S4亚族5个、S6亚族3个、S7亚族2个。结合这10个MYB基因表达和花瓣花青素苷含量变化结果,推测S4亚族ZeMYB32可能负调控花青素苷的合成,而ZeMYB3和ZeMYB16可能促进花青素苷的积累。此外,S6亚族ZeMYB9和ZeMYB10可能正调控花青素苷的合成。进一步研究发现,ZeMYB9定位于细胞核内。烟草中过表达ZeMYB9显著促进其叶片花青素苷的积累,花青素苷合成相关基因上调表达,表明ZeMYB9正向调控花青素合成。

关键词: 百日草, 花青素苷, MYB转录因子, 生物信息学分析

Abstract:

In this study,Zinnia elegans‘Dreamland Red’was employed as plant materials,and 33 MYB transcription factors were screened based on the transcriptome data of Z. elegans. Furthermore,phylogenetic tree analysis revealed that ten R2R3-MYB transcription factors probably related to anthocyanin synthesis were identified in Z. elegans. Among them,five MYB proteins of Z. elegans belonged to subgroup 4(S4),three MYB proteins were clustered in S6,and two MYB proteins were included in S7. According to combined analysis on gene expression levels of ten MYB genes and anthocyanidin content in Z. elegans‘Dreamland Red’,it was suggested that ZeMYB32 of S4 probably negatively regulated anthocyanin synthesis in Z. elegans,while ZeMYB3 and ZeMYB16 promoted the accumulation of anthocyanin. What’s more,ZeMYB9 and ZeMYB10 of S6 probably positively regulated anthocyanin synthesis. Further research found that ZeMYB9 is located in the nucleus. Overexpression of ZeMYB9 gene in tobacco enhanced the marked accumulation of anthocyanins in leaves,and dramatically up-regulated the expression levels of all detected genes related with anthocyanin synthesis,revealing that ZeMYB9 plays a positive role in anthocyanin biosynthesis.

Key words: Zinnia elegans, anthocyanin, MYB transcription factors, bioinformatics analysis

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