关键词: 茶树, 胁迫应答, 小分子热激蛋白, 基因克隆, 表达分析

Abstract: Four small heat shock protein（sHSP） genes were cloned from Camallia sinensis‘Longjing Changye’using RT-PCR technology. The open reading frames of them were 600，732，462 and 657 bp，which encoded 199，243，153 and 218 amino acids，respectively. According to the molecular weight of encoded protein，they were named as CsHSP22.4，CsHSP27.4，CsHSP17.5 and CsHSP25.2. Protein structure analysis showed that the four CsHSPs contained a ACD domain consisting of CRⅠ，CRⅡ and β6-loop，typically belonging to the sHSP family member. Subcellular localization prediction and phylogenetic analysis showed that CsHSP22.4 and CsHSP17.5 were mainly localized in the mitochondria and cytoplasm，belonging to the MⅠ and CⅠ subfamily，respectively. CsHSP27.4 and CsHSP25.2 were mainly located in the chloroplast，which belonged to the CP subfamily. qRT-PCR test results indicated that the expression of CsHSP22.4 and CsHSP27.4 was higher in leaf than in other tissues，while the expression of CsHSP17.5 and CsHSP25.2 was higher in stem. The expression of CsHSP22.4，CsHSP27.4，CsHSP17.5 and CsHSP25.2 was induced by heat and drought stress，and the expression level increased explosively under heat stress，implying that they were involved in response to heat and drought stress in Camallia sinensis，although they were more sensitive to heat stress.

Key words: tea plant, stress response, sHSP, gene cloning, expression analysis