园艺学报 ›› 2018, Vol. 45 ›› Issue (12): 2395-2406.doi: 10.16420/j.issn.0513-353x.2018-0324

• 研究论文 • 上一篇    下一篇



  1. 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室/农业部黄淮地区园艺作物生物学与种质创制重点开放实验室/山东果蔬优质高效生产协同创新中心,山东泰安 271018
  • 出版日期:2018-12-25 发布日期:2018-12-25
  • 基金资助:

Hydrogen Sulfide Interacting with Abscisic Acid Counteracts Oxidative Damages Against Chilling Stress in Cucumber Seedlings

LI Dandan,ZHANG Xiaowei,LIU Fengjiao,PAN Dongyun,and AI Xizhen*   

  1. College of Horticulture Science and Engineering,Shandong Agricultural University/State Key Laboratory of Crop Biology/Key Laboratory of Horticultural Crop Biology and Germplasm Innovation in Huanghuai Region of Agriculture Ministry/Collaborative Innovation Center of Shandong Province with High Quality and Efficient Production of Fruit and Vegetable,Tai’an,Shandong 271018,China
  • Online:2018-12-25 Published:2018-12-25

摘要: 为了探明硫化氢(H2S)与脱落酸(ABA)在提高植物耐冷性中的互作关系,以黄瓜幼苗为试材,叶面分别喷施1.0 mmol · L-1硫氢化钠(NaHS,H2S供体)、0.15 mmol · L-1次牛磺酸(HT,H2S清除剂)、50 μmol · L-1ABA、3 mmol · L-1 Na2WO4(钨酸钠,ABA合成抑制剂)、3 mmol · L-1 Na2WO4 + 1.0 mmol · L-1 NaHS、0.15 mmol · L-1 HT + 50 μmol · L-1 ABA及去离子水(H2O),研究低温(8 ℃昼/5 ℃夜)胁迫下H2S和ABA对黄瓜幼苗活性氧积累及抗氧化系统的影响。结果表明,低温胁迫48 h,NaHS和ABA预处理的幼苗冷害指数显著降低。ABA可以提高L–/D–半胱氨酸脱巯基酶(LCD/DCD)活性及其基因表达量,促进内源H2S产生;NaHS处理的9–顺式–环氧类胡萝卜素双加氧酶(NCED)活性及其基因相对表达也明显增加,内源ABA含量快速升高。低温下NaHS和ABA处理均可减少活性氧积累,提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性与基因相对表达量,增加抗坏血酸(AsA)和谷胱甘肽(GSH)含量及还原型/氧化型抗坏血酸(AsA/DHA)和还原型/氧化型谷胱甘肽(GSH/GSSG)比例,从而减轻低温胁迫对黄瓜幼苗的过氧化伤害。ABA合成抑制剂Na2WO4处理后,H2S对黄瓜幼苗抗氧化能力的促进效应明显减弱;同样,ABA对黄瓜幼苗活性氧的调控作用也被H2S清除剂HT所逆转。可见,H2S和ABA可诱导黄瓜幼苗耐冷性,二者在信号转导过程中存在交互效应和“对话”机制。

关键词: 黄瓜, 硫化氢, 脱落酸, 活性氧, 抗氧化系统, 低温

Abstract: In order to verify the interaction between hydrogen sulfide(H2S)and abscisic acid(ABA)in improving chilling tolerance of plants,we evaluated the effect of H2S and ABA on the reactive oxygen (ROS)accumulation and antioxidant system of cucumber(Cucumis sativa L.)seedlings subjected to chilling stress,that were pretreated with 1.0 mmol · L-1 sodium hydrosulfide(NaHS,H2S donor),0.15 mmol · L-1 hypotaurine(HT,H2S scavenger),50 μmol · L-1 ABA,3 mmol · L-1 sodium tungstate(Na2WO4,ABA inhibitor),3 mmol · L-1 Na2WO4 + 1.0 mmol · L-1 NaHS,0.15 mmol · L-1 HT + 50 μmol · L-1 ABA or deionized water(H2O)and the H2O pretreatment at room temperature was used as the control. The results showed that the cold injury index of NaHS- and ABA-pretreated seedlings decreased significantly after 48 h of chilling stress. ABA led to an increase in the activity and relative mRNA expression of L-/D-cysteine desulfhydrase(LCD/DCD),and consequently promoted the accumulation of endogenous H2S. Similarly,NaHS enhanced the 9-cis-epoxycarotenoid dioxygenase(NCED)activity and relative mRNA expression,and improved the endogenous ABA. NaHS and ABA reduced the accumulation of hydrogen peroxide(H2O2)and production rate of superoxide anion caused by chilling stress,whereas elevated the activities and relative mRNA expression of superoxide dismutase(SOD),peroxidase(POD),ascorbate peroxidase(APX)and glutathione reductase(GR),compared with the H2O-treated seedlings. Furthermore,NaHS and ABA led to an increase in the contents of ascorbic acid(AsA)and glutathione(GSH),as well as the ratio of reduced/oxidized ascorbic acid(AsA/DHA)and reduced/oxidized glutathione(GSH/GSSG)in stressed seedlings,thus resulting in the alleviation of oxidative damage. The H2S-induced positive effect on the antioxidant ability was weakened by addition of Na2WO4,a specific inhibitor of ABA biosynthesis. Similarly,the regulating role of ABA in the ROS metabolism in cucumber seedlings was also reversed by HT,a specific scavenger of H2S. All of the above-mentioned results suggest that H2S and ABA could improve the chilling tolerance of cucumber seedlings,and the two signal molecules have interaction and cross talk in the signal transduction.

Key words: cucumber, hydrogen sulfide, abscisic acid, reactive oxygen(ROS), antioxidant system, low temperature