关键词: 基因编辑, 园艺作物, 应用, 展望

Abstract:

Genome editing is a new technology based on programmable nucleases to make precise changes in the genomes of cells. These nucleases create site-specific double-strand breaks（DSBs）at desired locations in the genome. The induced double-strand breaks are repaired through nonhomologous end-joining（NHEJ）or homologous recombination（HR），resulting in targeted mutations including deletion，insertion and exchange of specific DNA fragment. Here，we explained the principle of ZNF，TALEN and CRISPR/Cas9 and mainly summarized their usage in plants，especially in horticultural plants. As a precise and efficient method of genetic engineering，genome editing was developed rapidly in recent years，which will be utilized widely to improve agronomic traits of plant in the future.

Key words: genome editing, horticultural crops, utilization, prospect