园艺学报 ›› 2021, Vol. 48 ›› Issue (6): 1250-1264.doi: 10.16420/j.issn.0513-353x.2020-0711
收稿日期:
2021-01-12
修回日期:
2021-03-25
出版日期:
2021-06-25
发布日期:
2021-07-08
通讯作者:
洪艳,戴思兰
E-mail:hongy@bjfu.edu.cn;silandai@sina.com
基金资助:
DENG Zeyi, SONG Xiang, HONG Yan(), DAI Silan()
Received:
2021-01-12
Revised:
2021-03-25
Online:
2021-06-25
Published:
2021-07-08
Contact:
HONG Yan,DAI Silan
E-mail:hongy@bjfu.edu.cn;silandai@sina.com
摘要:
综述了近30年来观赏植物转基因研究中使用的主要启动子类型,并对3类启动子(组成型启动子、组织特异性启动子和诱导型启动子)在基因工程中的应用效果进行了对比分析,深入探讨了观赏植物启动子研究和应用中存在的问题,以期为观赏植物基因工程研究中实现基因的高效特异表达提供研究思路。
中图分类号:
邓泽宜, 宋想, 洪艳, 戴思兰. 启动子在观赏植物基因工程中的应用综述[J]. 园艺学报, 2021, 48(6): 1250-1264.
DENG Zeyi, SONG Xiang, HONG Yan, DAI Silan. Applications of Promoters in the Genetic Engineering of Ornamental Plants:A Review[J]. Acta Horticulturae Sinica, 2021, 48(6): 1250-1264.
序号 No. | 启动子类型(比例) Promoter type(%) | 特异性 Specificity | 主要启动子名称 Key promoter | 改良性状(文献篇数) Improved trait(No. of articles) |
---|---|---|---|---|
1 | 组成型(80.1) Constitutive promoter | CaMV 35S、NOS、Actin、Ubi | 抗逆性Resistance(45)、花色Color(29)、花期Florescence(12)、瓶插期Longevity(7)、分枝Branching(6)、花香Perfume(5)、花型Type(5)、株高Height(5)、生根Root(3) | |
2 | 组织特异性(15.1) Tissue-specific promoter | 花器官 Flower | fbp1、CHS、F3H、DFR、ANS、MYB、DEF、GLO | 花色Color(9)、瓶插期Longevity(7)、花型Type(3) |
维管束 Vascular | rolC | 花色Color(2) | ||
根部 Root | mas2 | 提高根部虫害抗性Improve the resistance to root pest(1) | ||
3 | 诱导型(4.8) Inducible promoter | 非生物胁迫 Abiotic stress | rd29A、cor15a | 抗旱耐盐Drought and salt tolerance(2)、耐低温Low temperature resistance(1) |
衰老 Senescence | SAG12 | 降低对外源乙烯的敏感性、延缓衰老Reduce sensitivity to exogenous ethylene and extend the life circle(4) |
表1 不同类型启动子在观赏植物基因工程中的应用
Table 1 Applications of different promoter types on the genetic engineering of ornamental plants
序号 No. | 启动子类型(比例) Promoter type(%) | 特异性 Specificity | 主要启动子名称 Key promoter | 改良性状(文献篇数) Improved trait(No. of articles) |
---|---|---|---|---|
1 | 组成型(80.1) Constitutive promoter | CaMV 35S、NOS、Actin、Ubi | 抗逆性Resistance(45)、花色Color(29)、花期Florescence(12)、瓶插期Longevity(7)、分枝Branching(6)、花香Perfume(5)、花型Type(5)、株高Height(5)、生根Root(3) | |
2 | 组织特异性(15.1) Tissue-specific promoter | 花器官 Flower | fbp1、CHS、F3H、DFR、ANS、MYB、DEF、GLO | 花色Color(9)、瓶插期Longevity(7)、花型Type(3) |
维管束 Vascular | rolC | 花色Color(2) | ||
根部 Root | mas2 | 提高根部虫害抗性Improve the resistance to root pest(1) | ||
3 | 诱导型(4.8) Inducible promoter | 非生物胁迫 Abiotic stress | rd29A、cor15a | 抗旱耐盐Drought and salt tolerance(2)、耐低温Low temperature resistance(1) |
衰老 Senescence | SAG12 | 降低对外源乙烯的敏感性、延缓衰老Reduce sensitivity to exogenous ethylene and extend the life circle(4) |
性状类型 Type of trait | 改良性状 Improved trait | 启动子 Promoter | 总计 Total | |||
---|---|---|---|---|---|---|
CaMV 35S | NOS | Actin | Ubi | |||
花部性状 Flower traits | 花色 Color | 28 | 0 | 1 | 0 | 29 |
花期 Florescence | 12 | 0 | 0 | 0 | 12 | |
花香 Perfume | 5 | 0 | 0 | 0 | 5 | |
花型 Type | 5 | 0 | 0 | 0 | 5 | |
株型Plant type | 株高 Height | 4 | 0 | 1 | 0 | 5 |
分枝 Branching | 6 | 0 | 0 | 0 | 6 | |
生根 Root | 3 | 0 | 0 | 0 | 3 | |
抗逆性Resistance | 生物胁迫 Biological stress | 29 | 2 | 0 | 2 | 33 |
非生物胁迫 Abiotic stress | 11 | 1 | 0 | 0 | 12 | |
其他Others | 瓶插期 Longevity | 4 | 3 | 0 | 0 | 7 |
总计Total | 107 | 6 | 2 | 2 | 117 |
表2 应用组成型启动子改良观赏植物性状的文献数
Table 2 The number of literatures on trait improvement using constitutive promoters in ornamental plants
性状类型 Type of trait | 改良性状 Improved trait | 启动子 Promoter | 总计 Total | |||
---|---|---|---|---|---|---|
CaMV 35S | NOS | Actin | Ubi | |||
花部性状 Flower traits | 花色 Color | 28 | 0 | 1 | 0 | 29 |
花期 Florescence | 12 | 0 | 0 | 0 | 12 | |
花香 Perfume | 5 | 0 | 0 | 0 | 5 | |
花型 Type | 5 | 0 | 0 | 0 | 5 | |
株型Plant type | 株高 Height | 4 | 0 | 1 | 0 | 5 |
分枝 Branching | 6 | 0 | 0 | 0 | 6 | |
生根 Root | 3 | 0 | 0 | 0 | 3 | |
抗逆性Resistance | 生物胁迫 Biological stress | 29 | 2 | 0 | 2 | 33 |
非生物胁迫 Abiotic stress | 11 | 1 | 0 | 0 | 12 | |
其他Others | 瓶插期 Longevity | 4 | 3 | 0 | 0 | 7 |
总计Total | 107 | 6 | 2 | 2 | 117 |
改良性状 Improved trait | 启动子 Promoter | 来源 Origin | 组织特异性 Tissue specificity | 目的基因 Target gene | 受体植物 Recipient plant | 改良效果 Improvement | 参考文献 Reference |
---|---|---|---|---|---|---|---|
花色 Color | CHS | 月季Rosa spp. | 花瓣 Petal | F3'5'H | 菊花 Chrysanthemum× morifolium | 花积累飞燕草素,呈蓝色 Petals accumulate delphinidin and turn to bluish | Brugliera et al., |
东方百合 Lilium oriental‘Sorbonne' | 矮牵牛Petunia×hybrida 东方百合Lilium oriental‘Sorbonne' | Qi et al., | |||||
三花龙胆 Gentiana triflora | CHSir | 烟草Nicotiana tabacum | 花色变浅 Faintly colored | Nakatsuka et al., | |||
F3H | 菊花 Chrysanthemum×morifolium | F3'5'H A3'5'GT | 菊花Chrysanthemum× morifolium | 花积累飞燕草素,呈蓝色 Petals accumulate delphinidin and turn to bluish | Noda et al., | ||
F3'5'H | 花积累飞燕草素,从红变为紫色 Petals accumulate delphinidin and change from red to purple | Noda et al., | |||||
TfDEF | 蝴蝶草属 Torenia spp. | AtTCP3-SRDX | 蓝猪耳Torenia fournieri | 花色变浅 Faintly colored | Sasaki et al., | ||
TfDFR | 花色变浅 Faintly colored | ||||||
TfGLO | 花瓣边缘脱色 Decolorized border | ||||||
TfF3H | 花色变浅 Faintly colored | ||||||
改良性状 Improved trait | 启动子 Promoter | 来源 Origin | 组织特异性 Tissue specificity | 目的基因 Target gene | 受体植物 Recipient plant | 改良效果 Improvement | 参考文献 Reference |
花色 Color | PANS | 烟草Nicotiana tabacum | 花瓣 Petal | B-peru | 烟草Nicotiana tabacum | 花冠由浅粉色转变为深粉色到深红色 Flower changes from light pink to dark pink,then to deep red | Kim et al., |
mPAP1 | |||||||
Md-MYB10R6 | 苹果Malus×domestica | Md-MYB10R6 | 矮牵牛Petunia×hybrida | 花冠从白色变为紫色 Flower changes from white to purple | Boase et al., | ||
InMYB1 | 牵牛花 Ipomoea nil | RsMYB1 | 矮牵牛Petunia×hybrida | 花冠由白色变为粉色 Flower color changes from white to intense pink | Naing et al., | ||
花型 Type | TfDEF | 蝴蝶草属 Torenia spp. | 花瓣 Petal | AtTCP3-SRDX | 蓝猪耳Torenia fournieri | 花瓣波浪状 Wavy borders | Sasaki et al., |
TfDFR | 花瓣边缘较窄 Narrowed border | ||||||
TfGLO | 花瓣边缘锯齿状 Serrated petal margins | ||||||
TfF3H | 花瓣边缘波浪状 Wavy petal margins | ||||||
InMYB2 | 牵牛花 Ipomoea nil | MYB106-SRDX | 拟南芥Arabidopsis thaliana | 花瓣皱缩 Wrinkled petals | Azuma et al., | ||
MYB106-VP16 | 花瓣向内卷曲 Curled inwards | ||||||
CPG | 仙客来Cyclamen persicum | AtTCP3-RD CpTCP1B-RD AtSEP3-RD CpSEP3-RD | 蓝猪耳Torenia fournieri | 花瓣边缘锯齿状 Serrated flowers 花瓣波浪状 Wavy flowers | Kasajima et al., | ||
瓶插期 Longevity | fbp1 | 矮牵牛Petunia× hybrida | 花芽、花瓣 和雄蕊 Flower buds,petals and stamens | etr1-1 | 文心兰Oncidium ‘Sweet Sugar' | 阻止乙烯生物合成,延长瓶插寿命 Prevent ethylene biosynthesis and improve longevity | Raffeiner et al., |
杂交文心兰Burrageara‘Stefan Isler Lava Flow' | Winkelmann et al., | ||||||
长寿花Kalanchoe blossfeldiana‘Debbie' | Sanikhani et al., | ||||||
香石竹Dianthus caryophyllus‘Lena' | Bovy et al., | ||||||
风铃草Campanula | Sriskandarajah et al., Mibus et al., | ||||||
天竺葵Pelargonium zonale‘Katinka' | Gehl et al., |
表3 观赏植物花器官特异性启动子的特性、类型及其应用
Table 3 Characteristics,types and applications of flower organ-specific promoters in ornamental plants
改良性状 Improved trait | 启动子 Promoter | 来源 Origin | 组织特异性 Tissue specificity | 目的基因 Target gene | 受体植物 Recipient plant | 改良效果 Improvement | 参考文献 Reference |
---|---|---|---|---|---|---|---|
花色 Color | CHS | 月季Rosa spp. | 花瓣 Petal | F3'5'H | 菊花 Chrysanthemum× morifolium | 花积累飞燕草素,呈蓝色 Petals accumulate delphinidin and turn to bluish | Brugliera et al., |
东方百合 Lilium oriental‘Sorbonne' | 矮牵牛Petunia×hybrida 东方百合Lilium oriental‘Sorbonne' | Qi et al., | |||||
三花龙胆 Gentiana triflora | CHSir | 烟草Nicotiana tabacum | 花色变浅 Faintly colored | Nakatsuka et al., | |||
F3H | 菊花 Chrysanthemum×morifolium | F3'5'H A3'5'GT | 菊花Chrysanthemum× morifolium | 花积累飞燕草素,呈蓝色 Petals accumulate delphinidin and turn to bluish | Noda et al., | ||
F3'5'H | 花积累飞燕草素,从红变为紫色 Petals accumulate delphinidin and change from red to purple | Noda et al., | |||||
TfDEF | 蝴蝶草属 Torenia spp. | AtTCP3-SRDX | 蓝猪耳Torenia fournieri | 花色变浅 Faintly colored | Sasaki et al., | ||
TfDFR | 花色变浅 Faintly colored | ||||||
TfGLO | 花瓣边缘脱色 Decolorized border | ||||||
TfF3H | 花色变浅 Faintly colored | ||||||
改良性状 Improved trait | 启动子 Promoter | 来源 Origin | 组织特异性 Tissue specificity | 目的基因 Target gene | 受体植物 Recipient plant | 改良效果 Improvement | 参考文献 Reference |
花色 Color | PANS | 烟草Nicotiana tabacum | 花瓣 Petal | B-peru | 烟草Nicotiana tabacum | 花冠由浅粉色转变为深粉色到深红色 Flower changes from light pink to dark pink,then to deep red | Kim et al., |
mPAP1 | |||||||
Md-MYB10R6 | 苹果Malus×domestica | Md-MYB10R6 | 矮牵牛Petunia×hybrida | 花冠从白色变为紫色 Flower changes from white to purple | Boase et al., | ||
InMYB1 | 牵牛花 Ipomoea nil | RsMYB1 | 矮牵牛Petunia×hybrida | 花冠由白色变为粉色 Flower color changes from white to intense pink | Naing et al., | ||
花型 Type | TfDEF | 蝴蝶草属 Torenia spp. | 花瓣 Petal | AtTCP3-SRDX | 蓝猪耳Torenia fournieri | 花瓣波浪状 Wavy borders | Sasaki et al., |
TfDFR | 花瓣边缘较窄 Narrowed border | ||||||
TfGLO | 花瓣边缘锯齿状 Serrated petal margins | ||||||
TfF3H | 花瓣边缘波浪状 Wavy petal margins | ||||||
InMYB2 | 牵牛花 Ipomoea nil | MYB106-SRDX | 拟南芥Arabidopsis thaliana | 花瓣皱缩 Wrinkled petals | Azuma et al., | ||
MYB106-VP16 | 花瓣向内卷曲 Curled inwards | ||||||
CPG | 仙客来Cyclamen persicum | AtTCP3-RD CpTCP1B-RD AtSEP3-RD CpSEP3-RD | 蓝猪耳Torenia fournieri | 花瓣边缘锯齿状 Serrated flowers 花瓣波浪状 Wavy flowers | Kasajima et al., | ||
瓶插期 Longevity | fbp1 | 矮牵牛Petunia× hybrida | 花芽、花瓣 和雄蕊 Flower buds,petals and stamens | etr1-1 | 文心兰Oncidium ‘Sweet Sugar' | 阻止乙烯生物合成,延长瓶插寿命 Prevent ethylene biosynthesis and improve longevity | Raffeiner et al., |
杂交文心兰Burrageara‘Stefan Isler Lava Flow' | Winkelmann et al., | ||||||
长寿花Kalanchoe blossfeldiana‘Debbie' | Sanikhani et al., | ||||||
香石竹Dianthus caryophyllus‘Lena' | Bovy et al., | ||||||
风铃草Campanula | Sriskandarajah et al., Mibus et al., | ||||||
天竺葵Pelargonium zonale‘Katinka' | Gehl et al., |
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