关键词: 蜡梅, CpAGL6基因, 启动子, GUS基因

Abstract: The regulative sequence（1 266 bp）of the flower development-related gene CpAGL6 promoter was cloned from genomic DNA of Chimonanthus praecox by hiTAIL-PCR. Bioinformatics analysis revealed that the promoter sequence contained basic cis-elements，such as TATA-box and CAAT-box and many elements involved in the plant abiotic stress. In order to study the function of CpAGL6 promoter，a promoter-reporter vector was constructed，and introduced into tobacco by Agrobacterium-mediated method. Then the transgenic tobacco with GUS histochemical staining and quantitative detection of GUS enzyme activity were analyzed，the results showed that CpAGL6 promoter could drive the GUS gene exclusively express in leaves，stems and flowers of transgenic tobacco，and there were significant differences of GUS enzyme activity among the flowers in different stages，almost no expression in roots. GUS enzyme activity of transgenic plants have increased under different treatments，including darkness，gibberellin（GA3）and 4 ℃ low temperature. The results indicated that CpAGL6 promotermainly driven GUS reporter gene expression in floral organs and green organs or tissues of tobacco，playing a very important role in abiotic stress resistance.

Key words: Chimonanthus praecox, CpAGL6 gene, GUS gene, promoter