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园艺学报 ›› 2015, Vol. 42 ›› Issue (11): 2197-2205.doi: 10.16420/j.issn.0513-353x.2015-0492

• 蔬菜 • 上一篇    下一篇

茄子果实性状相关基因的QTL 定位

葛海燕,刘 扬,陈火英   

  1. 上海交通大学农业与生物学院,上海 200240
  • 出版日期:2015-11-25 发布日期:2015-11-25
  • 基金资助:

    国家自然科学基金青年科学基金项目(31301770);上海市科技兴农种业项目[沪农科种字(2013)第5 号]

QTL Analysis of Fruit-Associated Traits in Eggplant

GE Hai-yan,LIU Yang,and CHEN Huo-ying   

  1. School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China
  • Online:2015-11-25 Published:2015-11-25

摘要:

以果实性状差异显著的茄子(Solanum melongena L.)种质材料绿茄‘131’和上海本地长
茄‘141’为作图亲本,构建了237 个株系的F2 群体,采用450 对SSR 标记构建遗传图谱,定位茄子果
实单果质量、果长、果径、果形指数和果实花萼大小的QTL。研究结果表明:在亲本间表现多态性的SSR
标记为63 对,平均多态性比例为14%;共有53 个标记被分配到13 个连锁群上(12 条染色体),13 个连
锁群覆盖总长度为532.2 cM,标记间距范围为0.2 ~ 45.9 cM,平均距离为10.04 cM;共定位了9 个与果
实性状相关的QTL 位点(单果质量1 个,果长1 个,果径2 个,果形指数3 个,果实花萼大小2 个)。

关键词: 茄子, 遗传图谱, SSR, 果实性状

Abstract:

Two morphologically and molecularly distinguished advanced breeding lines,Lü Qie
(Solanum melongena L.)‘131’and Shanghai Qie(Solanum melongena L.)‘141’,were selected for
mapping parents. An F2 population including 237 individuals was obtained. A total of 450 pairs of SSR
markers were used to construct linkage map and therefore to locate QTL related to fruit weight,fruit
length,fruit diameter,fruit shape index and fruit calyx size. The results showed that only 63(14%)markers
showed polymorphism between two parents. A total of 53 SSR markers were mapped into 13 linkage
groups,including 12 chromsomes. The map spanned 532.2 cM. The distance between loci varied from 0.2
to 45.9 cM,with the average distance of 10.04 cM. Nine QTLs for fruit-related traits were detected,
including 1 QTL for fruit weight,1 QTL for fruit length,2 QTLs for fruit diameter,3 QTLs for fruit shape
index and 2 QTLs for fruit calyx size.

Key words: eggplant, linkage map, SSR, fruit trait

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