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园艺学报 ›› 2015, Vol. 42 ›› Issue (11): 2144-2152.doi: 10.16420/j.issn.0513-353x.2015-0261

• 果树 • 上一篇    下一篇

核桃硬壳发育期内果皮木质素与相关酶活性的#br# 变化

文 菁 1,赵书岗2,*,王红霞1,张志华1,*,李夕勃1   

  1. 1 河北农业大学山区研究所,河北保定 071001;2 河北农业大学生命科学学院,河北保定 071001
  • 出版日期:2015-11-25 发布日期:2015-11-25
  • 基金资助:

    国家科技基础性工作专项(2013FY111700-5);国家‘十二五’科技支撑计划项目(2013BAD14B0103)

Changes of Lignin Content and Its Related Enzyme Activities in Endocarp#br# During Walnut Shell Development Period

WEN Jing1,ZHAO Shu-gang2,*,WANG Hong-xia1,ZHANG Zhi-hua1,*,and LI Xi-bo1   

  1. 1Mountainous Areas Research Institute,Agricultural University of Hebei,Baoding,Hebei 071001,China;2College of
    Life Sciences,Agticultural University of Hebei,Baoding,Hebei 071001,China
  • Online:2015-11-25 Published:2015-11-25

摘要:

以‘赞美’和‘珍珠香’核桃(Juglans regia L.)果实为试材,采用间苯三酚–HCl 法观察
了内果皮木质化过程,测定了其发育过程中木质素、纤维素和酚类物质含量以及相关酶活性的变化。结
果表明:两个品种核桃硬壳木质化始于6 月初,6 月中旬初步成型,7 月下旬完成。从硬壳木质化至坚果
采收,木质素、纤维素和酚类物质含量均呈逐渐升高趋势,但酚类物质含量在采前略有下降,POD 和PAL
活性均呈下降趋势,PPO 活性整体变化不大。相关分析表明,核桃硬壳分化期木质素含量与纤维素含量
极显著正相关,酚类物质含量与木质素含量极显著正相关,与纤维素含量显著正相关;POD 活性与木质
素、纤维素、酚类物质含量极显著负相关,与PAL 活性极显著正相关。

关键词: 核桃, 硬壳, 木质素, 纤维素, PAL, POD, PPO

Abstract:

Lignification process was observed by the method of Phloroglucinol-HCl and content of
lignin,cellulose,polyphenol and the activity of related enzymes were measured during development with
the materials of walnut of‘Zanmei’and‘Zhenzhuxiang’. The results showed that lignification occurred
at beginning of June,already initial took shape in mid-June and completed in late July. From shell
lignification to harvest,the content of lignin,cellulose and polyphenol was increased,while polyphenol
content slightly decreased before harvest. The activities of both POD and PAL were decreased and PPO
varied little. The correlation analysis showed that lignin content was significant positive correlated with the
content of cellulose. Polyphenol content was significant positive correlated with the content of lignin.
Polyphenol content was positively correlated with cellulose. The activity of POD was significant negative
correlated with content of lignin,cellulose and polyphenol,but positively correlated with activity of
PAL.

Key words: walnut, shell, lignin, cellulose, PAL, POD, PPO

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