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园艺学报 ›› 2021, Vol. 48 ›› Issue (11): 2161-2170.doi: 10.16420/j.issn.0513-353x.2020-0731

• 研究论文 • 上一篇    下一篇

基于代谢组学分析‘秋香’核桃贮藏期间脂肪酸的变化

许海峰1, 陈新1, 张士刚2, 相昆1, 张美勇1, 徐颖1, 王贵芳1,*()   

  1. 1山东省果树研究所,国家果树种质核桃板栗资源圃,山东泰安 271000
    2泰安市农业农村局,山东泰安 271000
  • 收稿日期:2021-04-11 修回日期:2021-08-20 发布日期:2021-12-02
  • 通讯作者: 王贵芳 E-mail:gfwang05@163.com
  • 基金资助:
    山东省农业良种工程项目(2020LZGC09);山东省自然科学基金项目(ZR2020MC161)

Change of Fatty Acid During Storage Period of‘Qiuxiang’Walnut Based on Metabolomics Analysis

XU Haifeng1, CHEN Xin1, ZHANG Shigang2, XIANG Kun1, ZHANG Meiyong1, XU Ying1, WANG Guifang1,*()   

  1. 1Shandong Institute of Pomology,National Germplasm Repository Genebank for Walnut and Chestnut,Tai’an,Shandong 271000,China
    2Tai’an City Agricultural and Rural Bureau,Tai’an,Shandong 271000,China
  • Received:2021-04-11 Revised:2021-08-20 Published:2021-12-02
  • Contact: WANG Guifang E-mail:gfwang05@163.com

摘要:

以‘秋香’核桃为试材,设置常温贮藏1年(CS)、4 ℃低温贮藏1年(LS)和未贮藏(WS)3个处理,利用超高效液相色谱/质谱(Ultra high performance liquid chromatography/mass spectrometry,LC/MS)定性定量分析核仁脂肪酸。结果表明:常温贮藏与未贮藏比较组样品共筛选到差异代谢物969个,而低温贮藏与未贮藏比较组共筛选到975个;通过变量权重值筛选(VIP > 1),与未贮藏相比,常温贮藏核桃差异代谢物有35个,而低温贮藏的有31个,其中硬脂酸含量均降低,亚油酸和α-亚麻酸在常温贮藏核桃中明显降低,但油酸含量却稍有增加,在低温贮藏中油酸、亚油酸和α-亚麻酸无明显变化差异,此外,在常温贮藏核桃中还检测到泛醌类代谢物下调,而在低温贮藏中角质类物质增加。KEGG通路分析发现差异代谢物主要参与不饱和脂肪酸的合成及α-亚麻酸和亚油酸的代谢,还有部分参与角质和泛醌类物质的合成。代谢物之间相关性分析表明,不饱和脂肪酸与泛醌类物质存在正相关关系,硬脂酸与角质存在负相关关系。综上,常温贮藏核桃泛醌类物质降低,抗氧化能力下降,α-亚麻酸和亚油酸氧化减少,而低温贮藏可抑制α-亚麻酸和亚油酸氧化减少,并提高角质含量适应低温环境。

关键词: 核桃, 代谢组学, 贮藏温度, 脂肪酸, 不饱和脂肪酸

Abstract:

The‘Qiuxiang’walnuts were set up three treatments:climate storage for one year(CS),4 ℃ low-temperature storage for one year(LS)and without storage(WS). Then the fatty acids of walnut kernels were analyzed qualitatively and quantitatively using LC/MS. The results show that a total of 969 differential metabolites were obtained in the CS_WS comparison group,and a total of 975 differential metabolites were obtained in the LS_WS comparison group. Through further screening according to variable weight value(VIP > 1),the CS_WS and LS_WS have 35 and 31 different metabolites,respectively. Comparing with WS,the stearidonic acid is reduced in CS and LS,linoleic acid and α-linolenic acid are significantly reduced in CS,the oleic acid is slightly increased in CS,however,in LS,oleic acid,linoleic acid and α-linolenic acid have no significant difference. In addition,ubiquinone is down-regulated in CS,while keratinous substances are increased in LS. The analysis of KEGG pathway shows that differential metabolites are mainly involved in the synthesis of unsaturated fatty acids and the metabolism of α-linolenic acid and linoleic acid. In addition,some differential metabolites are involved in the synthesis of keratin and ubiquinone. The correlation analysis among differential metabolites shows that there is a positive correlation between unsaturated fatty acids and ubiquinone,and a negative correlation between stearidonic acid and keratin. In summary,the ubiquinone is decreased and the antioxidant capacity is reduced of walnut in natural storage,thereby leading to reduced α-linolenic acid and linoleic acid,but the walnut in cold storage at 4 ℃ can inhibit the reduction of α-linolenic acid and linoleic acid,in addition,it also increase the keratin content to adapt to low-temperature environment.

Key words: walnut, metabolomics, storage temperature, fatty acid, unsaturated fatty acid

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