桃自交亲和性的分子机制及遗传特性研究

摘要/Abstract

摘要： 以油桃（Prunus persicavar.necturina）‘中油5号’及其自交后代为试材，利用花柱S-RNase基因和花粉SFB基因的特异性引物进行PCR扩增，对扩增片段进行回收、克隆、测序和Blast分析，确定了‘中油5号’的自交不亲和基因型为S₁S_2(m)。序列分析结果显示，‘中油5号’桃S₁-RNase和S_2(m)-RNase与多个其他李属S-RNase基因氨基酸和核苷酸序列间都具有极高的相似性，SFB₁和SFB₂基因与多个其他李属SFB基因氨基酸和核苷酸序列间也都具有极高的相似性。进一步对‘中油5号’自交后代个体的S基因型进行检测，发现后代群体中存在3种S基因型，分别为S₁S₁、S₁S_2(m)和S_2(m)S_2(m)，且分离比为8︰13︰7，与预期的比例1︰2︰1差异不显著（χ² = 0.214 < χ²_0.05,2 = 5.991），说明S-RNase基因和SFB基因都遵循两因素的孟德尔遗传规律进行连锁分离。此外，基因型为S₁S₁和S_2(m)S_2(m)的纯合体的存在，表明桃花粉SFB₁和SFB₂基因均不能被自身花柱S-RNase所识别，这是由于SFB₁和SFB₂基因翻译提前终止所造成的，从而使‘中油5号’表现出自交亲和性。

关键词: 桃, 花柱S-RNase 基因, 花粉SFB 基因, 自交亲和

Abstract: PCR were performed with specific primer sets of Prunus S-RNase and SFB alleles using ‘Zhongyou 5’（Prunus persica var.necturina）as the template. The amplification products were extracted，cloned，sequenced and then Blast analysis，which were used to determine the S-genotype of this cultivar，S₁S_2(m). Sequence analysis showed that Prunus persica S₁-RNase and S_2(m)-RNase alleles have exceptionally high identities with other Prunus S-RNase alleles，and，SFB₁and SFB₂alleles have exceptionally high identities with other Prunus SFB alleles，too. The S-genotypes of self-pollinated progeny of‘Zhongyou 5’were detected，it is obviously that three classes were found in progeny，and the segregation ratio of S₁S₁，S₁S_2(m)and S_2(m)S_2(m) was 8︰13︰7，which did fit to the expected ratio that was 1︰2︰1（χ² = 0.214 < χ²_0.05,2 = 5.991）. This result showed that S-RNase and SFB alleles are tightly co-segregation according to the Mendel genetic model. Moreover，the existence of S₁S₁ and S_2(m)S_2(m)homozygotes suggested that SFB₁ and SFB₂ could not be cognized by self S-RNase，due to that the protein of these two SFB alleles were premature，which resulted in the‘Zhongyou 5’is self-compatible.

Key words: peach, S-RNase, SFB, self-compatibility

中图分类号:

S 662.1

许高歌, 吴华清, 吴俊, 王超, 齐开杰, 张绍铃. 桃自交亲和性的分子机制及遗传特性研究[J]. 园艺学报, 2012, 39(6): 1035-1044.

XU Gao-Ge, WU Hua-Qing, WU Jun, WANG Chao, QI Kai-Jie, ZHANG Shao-Ling. Molecular Mechanism and Genetic Characterization of Self-compatibility in Peach[J]. ACTA HORTICULTURAE SINICA, 2012, 39(6): 1035-1044.

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