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园艺学报 ›› 2010, Vol. 37 ›› Issue (7): 1057-1064.

• 果树 • 上一篇    下一篇

甘肃桃抗南方根结线虫性状的SRAP标记

刘 伟1,2,曹 珂2,王力荣2,*,张绍铃1,朱更瑞2,方伟超2,陈昌文2   

  1. (1南京农业大学园艺学院,南京 210095;2中国农业科学院郑州果树研究所,郑州 450009)
  • 收稿日期:2009-09-17 修回日期:2010-06-22 出版日期:2010-07-25 发布日期:2010-07-25
  • 通讯作者: 王力荣

SRAP Markers for Resistance to Nematode(Meloidogyne incognita)of Prunus kansuensis

LIU Wei1.2,CAO Ke2,WANG Li-rong2,*,ZHANG Shao-ling1,ZHU Geng-rui2,FANG Wei-chao2,and CHEN Chang-wen2   

  1. (1College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2Zhengzhou Fruit Research Institute,Chinese Acdemy of Agricultutal Sciences,Zhengzhou 450009,China)
  • Received:2009-09-17 Revised:2010-06-22 Online:2010-07-25 Published:2010-07-25
  • Contact: WANG Li-rong

摘要: 以桃野生近缘种红根甘肃桃(Prunus kansuensis)与贝蕾[P. persica(L.)Batsch.]杂交的BC1代190株后代为试材,采用SRAP分子标记进行遗传分析。通过筛选在亲本中扩增稳定且多态性丰富的53对SRAP引物进行后代分析,获得来自红根甘肃桃且符合1︰1分离比例的标记115个。应用Mapmaker/Exp 3.0分析软件构建了红根甘肃桃的SRAP分子连锁图谱。该图谱共8个连锁群,包含103个标记,其中102个SRAP标记、1个抗南方根结线虫(Meloidogyne incognita)标记,总图距994.2 cM,每个连锁群的平均长度为124.3 cM,标记间平均图距为9.6 cM。抗南方根结线虫标记Mi-redkansu位于第5连锁群,并获得与其紧密连锁的SRAP标记,其遗传距离为2.1 cM。以36株F2代群体作为验证,检验标记的准确性为91.7%。

关键词: 桃, 根结线虫, 遗传图谱, SRAP标记

Abstract: A linkage map was obtained using a 190 BC1 progeny{Prunus persica(L.)Batsch. × [P. persica(L.)Batsch. × P. kansuensis]}. The selected primers 53 SRAP with rich polymorphism and steady bands were tested in progeny. One hundred and fifteen markers in 1︰1 ratio χ2 test were integrated in the map with Mapmaker/Exp 3.0 software. The map is composed of 103 loci(102 SRAP,1 root-knot nematodes resistance traits) distributed in 8 linkage groups. The map covers 994.2 cM of the peach genome. The average length of 8 groups is 124.3 cM,the average distance between adjacent loci is 9.6 cM. SRAP markers linked to root-knot nematodes resistance gene,which genetic distance is 2.1 cM,were located in group 5. The 36 F2 progeny was verified the validity. The accuracy rate is 91.7%.

Key words: peach, root-knot nematode, genetic linkage map, SRAP marker

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