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园艺学报 ›› 2009, Vol. 36 ›› Issue (5): 731-736.

• 研究简报 • 上一篇    下一篇

大白菜DH群体TuMV抗性的QTL定位与分析

张晓伟1,2,原玉香2,3,王晓武3, 孙日飞3,武 剑3,谢从华1*,蒋武生2,姚秋菊2   

  1. (1华中农业大学园艺林学学院, 武汉430070; 2 河南省农业科学院园艺研究所, 郑州450002; 3中国农业科学院蔬菜花卉研究所, 北京100081)
  • 收稿日期:2008-10-25 修回日期:2009-04-24 出版日期:2009-05-25 发布日期:2009-05-25
  • 通讯作者: 谢从华

QTL Mapping for TuMV Resistance in Chinese Cabbage [Brassica campestris L. ssp. pekinensis (Lour. ) O lssom]

ZHANG Xiao-wei1,2,YUAN Yu-xiang2,3,WANG Xiao-wu3,SUN Ri-fei3,WU Jian3, XIE Cong-hua1*,JIANG Wu-sheng2,and YAO Qiu-ju2

  

  1. (1College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, China; 2Institute of Horticulture,Henan Academ y of Agricultural Sciences, Zhengzhou 450002, China; 3Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China )
  • Received:2008-10-25 Revised:2009-04-24 Online:2009-05-25 Published:2009-05-25
  • Contact: XIE Cong-hua1

摘要: 利用已构建的包含287个标记位点的遗传图谱的大白菜DH群体,采用多模型QTL作图的方法,通过苗期人工接种TuMV-C4株系对来自抗病亲本Y19593和感病亲本Y177-12的DH群体的TuMV抗性进行QTL分析。共检测到3个QTLs,分别位于R03、R04和R06连锁群上,解释的表型变异在10.5%~21.9%之间,3个QTLs可解释40.0%的表型总变异。其中位于R04上命名为Tu-2的QTL解释的表型变异最高,为21.9%,其余两个位于R03和R06上的QTLs,分别解释10.5%和14.5%的表型变异。

关键词: 大白菜, TuMV, QTL定位, DH群体

Abstract: One hundred and eighty-three doubled hap loid (DH) lines derived from the cross of two Chinese cabbage [Brassica campestris L. ssp. pekinensis (Lour. ) Olssom ] DH lines, Y177212 (TuMV-susceptible) and Y195293 ( TuMV-resistant) , were used to map quantitative trait loci (QTL) related to TuMV resistance. TuMV resistance was evaluated for young seedlings by inoculating true leaves with TuMV-C4 strain.Based on the reported genetic map with 287 loci, 3 QTLs were detected for TuMV resistance, localizing on linkage group s R03, R04 and R06, respectively. The most significant of the identified QTL locates on R04,named Tu-2, exp laining 21.9% of the phenotyp ic variance. The other two QTL s mapped on R03 and R06, explaining 10.5% and 14.5% of the phenotyp ic variance, respectively. The three QTL s together exp lained
40.0% of the TuMV resistance variance.

Key words: Chinese cabbage, Turnip mosaic virus (TuMV), QTL mapp ing, DH population

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