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园艺学报 ›› 2007, Vol. 34 ›› Issue (5): 1297-1300.

• 研究报告 • 上一篇    下一篇

核桃叶提取物对朱砂叶螨体内几种酶活性的影响

王有年1;苗沛芳2;曹 挥2;刘素琪2;师光禄1*   

  1. (1北京农学院,农业应用新技术北京市重点实验室,北京 102206; 2山西农业大学农学院, 山西太谷 030800)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-10-25 发布日期:2007-10-25

Effects of Extracts of Leaves of Juglans regia to Several Enzyme Systems in Tetranychus cinnabarinus

WANG You-nian1; MIAO Pei-fang2;CAO Hui2; LIU Su-qi2; SHI Guang-lu1*

  

  1. (1Key Laboratory of New Technology, Agricultural Application, Beijing University of Agriculture, Beijing 102206, China; 2College of Agronomy, Shanxi Agricultural University, Taigu, Shanxi 030800, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

摘要: 测定了核桃叶石油醚提取物对朱砂叶螨体内谷胱甘肽-S-转移酶、单胺氧化酶、Ca2+Mg2+-ATP酶、乙酰胆碱酯酶活性的影响。结果表明,核桃叶石油醚提取物处理朱砂叶螨后,螨体内谷胱甘肽-S-转移酶活性被激活;而乙酰胆碱酯酶、单胺氧化酶、Ca2+Mg2+-ATP酶的活力受到一定程度的抑制,这可能会引起神经传递的阻断,导致螨体的死亡。结果表明核桃叶作为新型植物源农药具有良好的开发前景。

关键词: 核桃, 朱砂叶螨, 酶活性, 植物农药, 萃取

Abstract: Several kinds of important enzyme systems inTetranychus cinnabarinus treated with petroleum ether extract of Juglans regia leaves were tested. The results indicated that the extracts of J. regia leaves had strongly acaricidal activities against T. cinnabarinus. The activities of glutathione-S-transferase in T. cinnabarinus were strongly induced, but the activities of acetylcholinease, monoamine oxidase and Ca2+Mg2+-ATPs were restrained. The result suggested that the petroleum ether extract of J. regia leaves may be the cause the transmit block of nerve in mites. The result from the experiment also indicated that the discovery had a good developing prospect for making full use of J. regia leaves as new resources of botanical acaricide.

Key words: Juglans regia, Tetranychus cinnabarinus, Enzyme activity, Botanic pesticides, Extraction