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园艺学报 ›› 2007, Vol. 34 ›› Issue (3): 591-596.

• 果树 • 上一篇    下一篇

利用RAPD和SCAR标记鉴定草莓品种

王志刚1,2; 张志宏1*; 李 贺1; 高秀岩1; 杜国栋1; 谭昌华1
  

  1. (1 沈阳农业大学园艺学院, 沈阳110161; 2 辽宁省农业科学院花卉研究所, 沈阳110161)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-06-25 发布日期:2007-06-25

Identification of Strawberry Cultivars by RAPD and SCAR Markers

WANG Zhi-gang1,2;ZHANG Zhi-hong1*; L IHe1;GAO Xiu-yan1;DU Guo-dong1;TAN Chang-hua1   

  1. ( 1College of Horticulture, Shenyang AgriculturalUniversity, Shenyang 110161, China; 2 Institute of Floriculture, Liaoning Academ y of Agricultural Sciences, Shenyang 110161, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

摘要: 利用RAPD和SCAR标记对32份草莓材料进行了鉴定, 结果表明: 8个RAPD引物共扩增出
85个标记, 其中71个为多态性标记, 多态性比率为83。5%。25份草莓材料的RAPD图谱差异较大, 易于
区分。利用具有多态性的RAPD标记, 对28份草莓试材的亲缘关系进行分析, 初步鉴定出同名异物和同物
异名品种。两个RAPD标记被转化为片段长度分别为378 bp和214 bp的显性SCAR标记, 其多态性与相应
RAPD标记一致。利用这两个SCAR标记对草莓品种进行了初步鉴定。

关键词: 草莓, 品种鉴定, RAPD, SCAR

Abstract: Thirty-two strawberry cultivarswere tested by randomly amp lified polymorphic DNA (RAPD)
and sequence characterized amp lified region ( SCAR) markers. Results showed that 8 RAPD p rimers p roduced
85 bands, and 71 of them (83。5% ) were polymorphic bands. Among the 32 strawberrymaterials, 25 materi
alswere easy to be distinguished by RAPD markers. The relationship of 28 materialswas analyzed with RAPD
markers, and the synonyms and homonyms cultivars were screened p rimarily. Two dominant SCAR markers
WS01 andWS02 with the length of 378 bp and 214 bp respectively were developed from RAPD markers and
showed the same p rofiles as the corresponding RAPD markers. This study laid a sound foundation for the iden
tification of strawberry cultivar atmolecular level.

Key words: Strawberry, Cultivar identification, RAPD, SCAR