园艺学报 ›› 2006, Vol. 33 ›› Issue (6): 1349-1352.

• 研究报告 • 上一篇    下一篇



  1. (1 东北农业大学农学院, 黑龙江哈尔滨150030; 2 中国农业科学院蔬菜花卉研究所, 北京100081)
  • 收稿日期:2005-12-15 修回日期:2006-08-08 出版日期:2006-12-25 发布日期:2006-12-25
  • 通讯作者: 金黎萍

Genetic Diversity Analysis of Some Chinese Cultivated Potato Varieties Using AFLP Markers

Di Hong1;Chen Yili1;Jin Liping2   

  1. (1College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; 2 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
  • Received:2005-12-15 Revised:2006-08-08 Online:2006-12-25 Published:2006-12-25
  • Contact: Jin Liping

摘要: 采用AFLP方法分析中国各地79个马铃薯栽培品种, 获得了8对引物组合的扩增谱带, 为马铃薯品种的鉴定提供了分子依据。8对引物组合扩增结果共获得801条带, 平均每对引物组合产生100.13条带。其中493条为多态性条带, 平均多态性检出率为61.2% , 引物组合E11 /M51扩增的多态性比率最高。聚类分析将材料分成3类, 聚类结果与地域无明显相关。

关键词: 马铃薯, 遗传多样性, AFLP

Abstract: Amplification fragment length polymorphism (AFLP) analysiswas applied to study the genetic relationship among some of 79 Chinese cultivated potato varieties. 8 pairs of primers were screened to amplify and 801 bands were obtained, and the average number of band amplified per pair primer was 100.13. 493 of the bands presented polymorphism, the average rate of certificated polymorphism was 61.2%. The polymorphic value was the highest if amplified with the combined primer E11 /M51. Accessionswere classified into 3
groups according to the analysis of clustering. There was no obvious difference in geographic relationship among the clustering results. In addition, the potato accessions could be certified through AFLP molecular markers.

Key words: Potato, Genetic diversity, AFLP