园艺学报 ›› 2006, Vol. 33 ›› Issue (6): 1209-1214.

• 研究论文 • 上一篇    下一篇


申书兴1, 2*;侯喜林1;张成合2   

  1. (1 南京农业大学作物遗传与种质资源创新国家重点实验室, 园艺学院, 江苏南京210095; 2 河北农业大学园艺学院,河北保定071001)
  • 收稿日期:2006-04-07 修回日期:2006-09-05 出版日期:2006-12-25 发布日期:2006-12-25

A Study on Obtaining Primary Trisomics by the Isolated Microspore Cultureof Autotetraploid Chinese Cabbage

Shen Shuxing 1, 2*;Hou Xilin1;Zhang Chenghe2   

  1. (1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultral University,Nangjing, Jiangsu 210095, China; 2College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071001, China)
  • Received:2006-04-07 Revised:2006-09-05 Online:2006-12-25 Published:2006-12-25

摘要: 创建大白菜初级三体是开展基因染色体定位等细胞分子遗传学方面研究的基础。本研究从四倍体大白菜9405自交系游离小孢子培养获得的植株中, 筛选出79个2n + 1植株。通过根尖中期染色体核型分析, 并结合减数分裂和植株外部形态特征观察, 从中鉴定筛选出了一整套初级三体。各初级三体在植株的形态特征上表现出不同程度的差异。四倍体大白菜游离小孢子培养是创建初级三体的快捷简便的有效途径。

关键词: 大白菜, 小孢子, 三体, 核型

Abstract: Selection of primary trisomics of Chinese cabbage 〔Brassica campestris L. ssp. pekinesis (Lour) Olsson〕is an important foundation for the gene chromosome mapp ing and other genetic studies. The aneuploid plants with one extra chromosome (2n + 1) were obtained from the isolatedmicrospore cultured plantlets of the tetraploid Chinese cabbage. By karyotype analysis of the root tip metaphase chromosome, meiosis observation and plant characters investigation, a set of primary trisomics ( Tri-1, Tri-2, Tri-3, Tri-4, Tri-5, Tri-6, Tri-7, Tri-8, Tri-9 and Tri-10) was identified. Each trisomic was with some unique characters on plant morphological traits. The isolated microspore culture of tetraploid Chinese cabbage is a convenient and effective way to select trisomics in Chinese cabbage.

Key words: Chinese cabbage, Brassica campestris L. ssp. pekinesis (Lour) Olsson, Microspore, Risomics, Karyotype