杏和李树啤酒花矮化类病毒的检测与序列分析

园艺学报 ›› 2006, Vol. 33 ›› Issue (6): 1193-1198.

杏和李树啤酒花矮化类病毒的检测与序列分析

杨元爱^{1, 2};李世访^2*;成卓敏²;王红清^1*

(¹ 中国农业大学农学与生物技术学院果树系, 北京100094; ² 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室, 北京100094)

收稿日期:2006-06-21 修回日期:2006-08-21 出版日期:2006-12-25 发布日期:2006-12-25

Detection and Sequence Analysis of Hop stunt viroid in Apricot and Plum

Yang Yuanai^{1, 2};Li Shifang^2*;Cheng Zhuomin²;Wang Hongqing^1*

(¹Department of Fruit Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China;²State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China)

Received:2006-06-21 Revised:2006-08-21 Online:2006-12-25 Published:2006-12-25

摘要/Abstract

摘要： 从无明显症状表现的24个杏样品, 37个李样品的1年生枝条表皮中抽提低分子RNA进行DIG-cDNA斑点杂交、RT-PCR、正反向聚丙烯酰胺凝胶电泳(Return-PAGE) 和生物学检测。结果表明: 杏有3个样品, 李有6个样品带有啤酒花矮化类病毒(Hop stunt viroid, HSVd) 。从这9个样品中选a5、a17、p6、p16 进行克隆和测序分析。所得序列与GenBank中已报道的HSVd序列同源性达99.0% ～99.7% , 且序列变异与地域、寄主等无明显相关性, 说明HSVd的序列是比较保守的。

关键词: 啤酒花矮化类病毒, 检测, 杏, 李

Abstract: Low molecular mass RNAs extracted from bark of one year old branch of 24 apricot samples, 37 plum samples were used for DIG-cDNA Dot-blot hybridization, RT-PCR, Return-PAGE and biological indexing. There were no clear symptoms when these samples were collected. The result showed that 3 of 24 apricot samples, 6 of 37 plum samples were infected with Hop stunt viroid (HSVd). a5, a17, p6, p16 selected from the 9 samples were used for cloning and sequencing analysis. HSVd sequence variants obtained were
compared with HSVd reported in GenBank and showed 99.0% - 99.7% identity. There was no clear relativity among sequence variants, region and host. These results suggest that sequence of HSVd is relatively conserved.

Key words: Hop stunt viroid, Detection, Apricot, Plum

中图分类号:

杨元爱;;李世访;成卓敏;王红清. 杏和李树啤酒花矮化类病毒的检测与序列分析[J]. 园艺学报, 2006, 33(6): 1193-1198.

Yang Yuanai;;Li Shifang;Cheng Zhuomin;Wang Hongqing. Detection and Sequence Analysis of Hop stunt viroid in Apricot and Plum[J]. ACTA HORTICULTURAE SINICA, 2006, 33(6): 1193-1198.

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