园艺学报 ›› 2006, Vol. 33 ›› Issue (6): 1193-1198.

• 研究论文 • 上一篇    下一篇


杨元爱1, 2;李世访2*;成卓敏2;王红清1*   

  1. (1 中国农业大学农学与生物技术学院果树系, 北京100094; 2 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室, 北京100094)
  • 收稿日期:2006-06-21 修回日期:2006-08-21 出版日期:2006-12-25 发布日期:2006-12-25

Detection and Sequence Analysis of Hop stunt viroid in Apricot and Plum

Yang Yuanai1, 2;Li Shifang2*;Cheng Zhuomin2;Wang Hongqing1*

  1. (1Department of Fruit Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China;2 State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China)
  • Received:2006-06-21 Revised:2006-08-21 Online:2006-12-25 Published:2006-12-25

摘要: 从无明显症状表现的24个杏样品, 37个李样品的1年生枝条表皮中抽提低分子RNA进行DIG-cDNA斑点杂交、RT-PCR、正反向聚丙烯酰胺凝胶电泳(Return-PAGE) 和生物学检测。结果表明: 杏有3个样品, 李有6个样品带有啤酒花矮化类病毒(Hop stunt viroid, HSVd) 。从这9个样品中选a5、a17、p6、p16 进行克隆和测序分析。所得序列与GenBank中已报道的HSVd序列同源性达99.0% ~99.7% , 且序列变异与地域、寄主等无明显相关性, 说明HSVd的序列是比较保守的。

关键词: 啤酒花矮化类病毒, 检测, 杏,

Abstract: Low molecular mass RNAs extracted from bark of one year old branch of 24 apricot samples, 37 plum samples were used for DIG-cDNA Dot-blot hybridization, RT-PCR, Return-PAGE and biological indexing. There were no clear symptoms when these samples were collected. The result showed that 3 of 24 apricot samples, 6 of 37 plum samples were infected with Hop stunt viroid (HSVd). a5, a17, p6, p16 selected from the 9 samples were used for cloning and sequencing analysis. HSVd sequence variants obtained were
compared with HSVd reported in GenBank and showed 99.0% - 99.7% identity. There was no clear relativity among sequence variants, region and host. These results suggest that sequence of HSVd is relatively conserved.

Key words: Hop stunt viroid, Detection, Apricot, Plum