园艺学报 ›› 2003, Vol. 30 ›› Issue (6): 687-689.

• 研究论文 • 上一篇    下一篇



  1. (山东农业大学农学院,泰安271018)
  • 收稿日期:2002-12-23 修回日期:2003-03-10 出版日期:2003-12-25 发布日期:2003-12-25

Detection of Potato Virus X by Reverse~ dnscription Polymerase Chain Reaction

Sun Qi and Zhang Chunqing   

  1. (/Ig阳nom, College,Stmnd~ngAgricuturcd Univershy, Tai'an 271018, Ch/na)
  • Received:2002-12-23 Revised:2003-03-10 Online:2003-12-25 Published:2003-12-25

摘要: 通过对“一步法”RNA提取方法的改进,简化了提取程序,并保证了RNA的质量。根据马铃
薯x病毒(PVX)基因序列设计合成了一对特异性引物,运用反转录PCR (RT-PCR)对PVX-RNA进行扩
增,成功得到了与预期大小相一致的308 bp片段,而对照未得到任何产物,同时对反转录酶AMV用量做了

关键词: 马铃薯x病毒, 病毒, 检测, 反转录PCR

Abstract: T0tal RNA was extracted with an impmvod one.step method from tobacco leaves infec ted with potato
virus X (P、Ⅸ ).The mole quality of tested RNA was very good,which indieated that the impmvod method was
very efective and simple. A pair of specific primers were designed an d synthesized based on the homologous
nueleotide sequence of diferent isolates of PVX by the reverse transcription an d polymerase chain reaction (RT.
PCR),a 308 bp DNA fragment was amplified successfully from PVX of Shandong isolate,and no fragment was
obtained from contro1.With only 1U Reverse Transefiptase XL (AMV),the am plified resnlt were still very good.
The resnlts showed that an economical an d convenient RT.PCR detec tion system of Pvx was set up.

Key words: :Pvx (Pomto vires X), Vires, Detection, Reverse transcription and polymemse chain reaction(RT.PCR)