基于SSR分子标记的新疆桃地方品种遗传多样性分析及DNA指纹图谱的构建

doi:10.16420/j.issn.0513-353x.2025-0698

园艺学报 ›› 2026, Vol. 53 ›› Issue (8): 2224-2240.doi: 10.16420/j.issn.0513-353x.2025-0698

• 遗传育种 · 种质资源 · 分子生物学 • 上一篇下一篇

基于SSR分子标记的新疆桃地方品种遗传多样性分析及DNA指纹图谱的构建

买尔哈巴·塞来江¹^,²^,^*, 程平³^,^*, 米尔阿迪力·麦木提敏⁴, 李丹³, 艾沙江·阿不都沙拉木¹^,²^,^**()

¹ 喀什大学生命与地理科学学院，新疆喀什 844006
² 新疆帕米尔高原生物资源与生态重点实验室，新疆喀什 844006
³ 新疆农业大学林学与风景园林学院，乌鲁木齐 830052
⁴ 喀什地区科技局，新疆喀什 844006

收稿日期:2025-12-17 修回日期:2026-07-09 出版日期:2026-08-25 发布日期:2026-08-25
通讯作者:
^** E-mail：aysajanxj@sina.com
作者简介:
* 共同第一作者
基金资助:
新疆维吾尔自治区重大科技专项(2023A02008-4); 喀什大学校级科研项目[(2024)

Genetic Diversity Analysis and DNA Fingerprinting Development of Prunus ferganensis Landrace Germplasm Based on SSR

SAILAIJIANG Maierhaba¹^,², CHENG Ping³, MAIMUTIMIN Mieradili⁴, LI Dan³, ABDUSALAM Aysajan¹^,²^,^**()

¹ College of Life and Geographic Sciences， Kashi University，Kashi， Xinjiang 844006， China
² Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang， Kashi， Xinjiang 844006， China
³ College of Forestry and Landscape Architecture， Xinjiang Agricultural University， Urumqi 830052， China
⁴ Kashgar Prefectural Science and Technology Bureau， Kashi， Xinjiang 844006， China

Received:2025-12-17 Revised:2026-07-09 Published:2026-08-25 Online:2026-08-25

摘要/Abstract

摘要：

目前对新疆桃地方种质资源的系统性遗传评价仍较为缺乏。选取153份新疆桃地方种质作为研究对象，利用16对SSR引物开展多维度分析（遗传多样性、聚类分析及群体结构分析），系统解析其遗传变异规律。同时，基于毛细管电泳分型技术，成功构建了新疆桃地方品种DNA指纹图谱。主要结果如下：（1）16对SSR引物在153份桃种质中共扩增出121个多态性位点；有效等位基因数（N_e）范围为1.10 ~ 4.70；香农信息指数（I）范围为0.23 ~ 1.78；观测杂合度（H_o）为0.05 ~ 0.26；期望杂合度（H_e）为0.09 ~ 0.79；多态信息含量（PIC）为0.09 ~ 0.76（均值0.50）。（2）UPGMA聚类分析将153份种质资源分为9个类群，平均遗传距离为0.50；聚类结果与品种地理来源及桃类型无明显相关性。（3）基于STRUCTURE群体遗传结构分析表明，153份种质可分为3个亚群，推测其可能分别对应野生型、地方品种及栽培品种三大遗传背景。（4）采用覆盖桃基因组8条染色体的SSR核心引物组合，成功构建128份新疆桃地方种质资源的分子身份识别编码体系，为核心种质库的标准化构建及优异基因挖掘奠定了分子鉴定基础。

关键词: 桃, 新疆桃, 种质, SSR标记, 遗传多样性, 指纹图谱

Abstract:

Currently，systematic genetic evaluation of Xinjiang peach landrace germplasm remains limited. A total of 153 Xinjiang peach landrace accessions were selected and analyzed using 16 SSR primers for multi-dimensional analyses（genetic diversity，cluster analysis，and population structure analysis）to systematically unravel their genetic variation patterns. Meanwhile，a DNA fingerprint database for Xinjiang peach landrace varieties was successfully constructed based on capillary electrophoresis. The main results are as follows：(1) The 16 SSR primer pairs amplified 121 polymorphic loci across the 153 peach accessions；the effective number of alleles（N_e）ranged from 1.10 to 4.70，Shannon’s information index（I）from 0.23 to 1.78，observed heterozygosity（H_o）from 0.05 to 0.26，expected heterozygosity （H_e）from 0.09 to 0.79，and polymorphism information content（PIC）from 0.09 to 0.76（mean 0.50）. (2) UPGMA cluster analysis divided the 153 accessions into 9 groups with an average genetic distance of 0.50；the clustering showed no significant correlation with geographical origin or peach type. (3) STRUCTURE-based population genetic structure analysis indicated that the 153 accessions could be partitioned into three subpopulations，which were speculated to correspond to wild types，landraces，and cultivated cultivars. (4) Using a core set of SSR primers covering the eight chromosomes of the peach genome，molecular identification codes were successfully established for 128 Xinjiang peach landrace accessions，providing a molecular basis for the standardized construction of core germplasm collections and the mining of elite genes.

Key words: Prunus persica, Prunus ferganensis, germplasm, SSR markers, genetic diversity, DNA fingerprinting

买尔哈巴·塞来江, 程平, 米尔阿迪力·麦木提敏, 李丹, 艾沙江·阿不都沙拉木. 基于SSR分子标记的新疆桃地方品种遗传多样性分析及DNA指纹图谱的构建[J]. 园艺学报, 2026, 53(8): 2224-2240.

SAILAIJIANG Maierhaba, CHENG Ping, MAIMUTIMIN Mieradili, LI Dan, ABDUSALAM Aysajan. Genetic Diversity Analysis and DNA Fingerprinting Development of Prunus ferganensis Landrace Germplasm Based on SSR[J]. Acta Horticulturae Sinica, 2026, 53(8): 2224-2240.

图/表 10

参考文献 57

[1]	An Jiale, Yu Xinchun, Tian Yi, Zhang Xueying, Chen Haijiang. 2025. SSR analysis of genetic relationship in peach new strain‘Baojiazaomi’. Molecular Plant Breeding, 23 (1):134-141. (in Chinese)
	安佳乐, 于辛春, 田义, 张学英, 陈海江. 2025. 桃新品系‘保佳早蜜’亲缘关系的SSR分析. 分子植物育种, 23 (1):134-141.
[2]	Antanynienė R, Šikšnianienė J B, Stanys V, Frercks B. 2023. Fingerprinting of plum(Prunus domestica)genotypes in Lithuania using SSR markers. Plants (Basel), 12 (7):1538.
[3]	Aranzana M J, Garcia-M J, Carbó J. 2002. Development and variability analysis of microsatellite markers in peach. Plant Breeding, 121 (1):87-92. doi: 10.1046/j.1439-0523.2002.00656.x URL
[4]	Aranzana M, Carbó J, Arús P. 2003. Microsatellite variability in peach[Prunus persica(L.) Batsch]:cultivar identification,marker mutation,pedigree inferences and population structure. Theoretical and Applied Genetics, 106:1341-1352. pmid: 12750778
[5]	Chen Jianjun, Wang Yuan, Ou Qiaoming, Wang Hong, Zhao Xiumei, Wang Falin. 2013. Genetic diversity of local peach germplasm resources in Gansu. Acta Agriculturae Boreali-Occidentalis Sinica, 22 (7):149-155. (in Chinese)
	陈建军, 王玉安, 欧巧明, 王鸿, 赵秀梅, 王发林. 2013. 甘肃地方桃种质资源的遗传多样性. 西北农业学报, 22 (7):149-155.
[6]	Delectis Florae Reipublicae Popularis Sinicae Agendae Academiae Sinicae Edita. 1986. Flora Reipublicae Popularis Sinicae. Tomus 38. Beijing:Science Press:20. (in Chinese)
	中国科学院中国植物志编辑委员会. 1986. 中国植物志. 38卷. 北京:科学出版社:20.
[7]	Diego M, Teresa D M, Sabrina M, Valeria A, Igor P, Cassia D S L. 2015. Whole-genome analysis of diversity and snp-major gene association in peach germplasm. PLoS ONE, 10 (9):e0136803. doi: 10.1371/journal.pone.0136803 URL
[8]	Dirlewanger E, Cosson P, Tavaud M, Aranzana J, Poizat C, Zanetto A, Arús P, Laigret F. 2002. Development of microsatellite markers in peach [Prunus persica(L.)Batsch] and their use in genetic diversity analysis in peach and sweet cherry(Prunus avium L.). Theoretical and Applied Genetics, 105 (1):127-138. pmid: 12582570
[9]	Du Jingjing, Liu Guoyin, Wei Junya, Liu Debing, Yang Xiaozhen. 2013. Construction of molecular ID for grape germplasm resources based on SSR markers. Bulletin of Botanical Research, 33 (2):232-237. (in Chinese) doi: 10.7525/j.issn.1673-5102.2013.02.019
	杜晶晶, 刘国银, 魏军亚, 刘德兵, 杨小振. 2013. 基于SSR标记构建葡萄种质资源分子身份证. 植物研究, 33 (2):232-237. doi: 10.7525/j.issn.1673-5102.2013.02.019
[10]	Duan Yonghong, Yang Xin, Yu Guanqun, Xia Junjun, Song Lushuai, Bai Xiaodong, Peng Suotang. 2025. Genetic diversity and principal component analysis of 125 potato germplasm resources. Biotechnology Bulletin, 41 (6):130-143. (in Chinese) doi: 10.13560/j.cnki.biotech.bull.1985.2024-1285
	段永红, 杨欣, 于冠群, 夏俊俊, 宋陆帅, 白小东, 彭锁堂. 2025. 125份马铃薯种质资源遗传多样性及主成分分析. 生物技术通报, 41 (6):130-143. doi: 10.13560/j.cnki.biotech.bull.1985.2024-1285
[11]	Ge Zhigang, Yu Mingliang, Ma Ruijuan, Shen Zhijun. 2010. Analysis of genetic diversity and relationship of flat peach cultivars by SSR. Journal of Fruit Science, 27 (3):356-361. (in Chinese)
	葛志刚, 俞明亮, 马瑞娟, 沈志军. 2010. 蟠桃种质SSR标记的遗传多样性分析. 果树学报, 27 (3):356-361.
[12]	Guan Liping, Wang Lingling, Cao Ke, Wang Lirong. 2021. SSR core primer screening and application for peach cultivar identification. China Fruits,(6):33-38. (in Chinese)
	关利平, 王玲玲, 曹珂, 王力荣. 2021. 桃品种鉴定的SSR核心引物筛选及其应用. 中国果树,(6):33-38.
[13]	Guo Shaolei. 2019. Genetic mapping and regulatory gene analysis of firmness trait in peach fruit[Ph. D. Dissertation]. Nanjing: Nanjing Agricultural University. (in Chinese)
	郭绍雷. 2019. 桃果实硬质性状的遗传定位及调控基因分析[博士论文]. 南京: 南京农业大学.
[14]	Hou Jiayin, Feng Shuxiang, Dai Songhua, Yan Shufang. 2023. TP-M13-SSR molecular marker identification and genetic relationship analysis of seven new ornamental peach germplasms. Journal of Beijing Forestry University, 45 (8):132-141. (in Chinese)
	侯佳音, 冯树香, 代嵩华, 闫淑芳. 2023. 7种观赏桃新种质的TP-M13-SSR分子标记鉴定及亲缘关系分析. 北京林业大学学报, 45 (8):132-141.
[15]	Kumar R, Dimri D C, Karki K, Rai K M, Sing N K, Shivran J S, Bharti S. 2023. SSR marker based profiling and population structure analysis in peach(Prunus persica)germplasm. The Indian Journal of Agricultural Sciences, 93 (10):1080-1085. doi: 10.56093/ijas.v93i10.132658 URL
[16]	Li A, Sun S, Wang H, Badrunnesa A, Meng J, Li X, Gao Y, Niu L, Pan L, Duan W, Cui G, Wang Z, Zeng W. 2025a. Peach MD: a multi-omics database for peach. Molecular Horticulture, 5 (1):37.
[17]	Li Dan, Bai Lixia, Tang Zhuoyue, Yang Kanghui, Yang Lu, Cheng Ping. 2025. Diversity analysis of fruit traits in 105 peach germplasm resources from Xinjiang. Journal of Fruit Science, 42 (7):1377-1388. (in Chinese)
	李丹, 白丽霞, 唐卓越, 杨康慧, 杨璐, 程平. 2025. 新疆105份桃种质资源果实性状多样性分析. 果树学报, 42 (7):1377-1388.
[18]	Li Xianghua, Han Xiaochong, Lu Ying, Li Hanyu, Hou Jian, Sun Yanpeng, Han xiaoyang. 2025. Genetic diversity analysis of traditional tea tree resources in Tai’an based on SSR molecular markers. Shandong Agricultural Sciences, 57 (5):69-74. (in Chinese)
	李相花, 韩笑翀, 鹿英, 李涵宇, 侯剑, 孙衍鹏, 韩晓阳. 2025. 基于SSR分子标记的泰安老茶树资源遗传多样性分析. 山东农业科学, 57 (5):69-74.
[19]	Li Xuesong, Hua Rong, Sun Dafeng, Yue Wansong, Zhang Junbo, Li Jianying, Liu Shaoxiong. 2025. Development of SSR molecular markers and construction of fingerprint codes for Tremella aurantialba. Acta Horticulturae Sinica, 52 (7):1780-1788. (in Chinese)
	李雪松, 华蓉, 孙达锋, 岳万松, 张俊波, 李建英, 刘绍雄. 2025. 金耳SSR分子标记开发及指纹编码构建. 园艺学报, 52 (7):1780-1788. doi: 10.16420/j.issn.0513-353x.2024-0767
[20]	Li Y, Arús P, Wu J L, Zhu G R, Fang W C, Chen C W, Wang X W, Cao K, Wang L R. 2025b. Panvariome and pangenome of 1 020 global peach accessions shed light on evolution patterns,hidden natural variations,and efficient gene discovery. Molecular Plant, 18 (6):995-1013. doi: 10.1016/j.molp.2025.04.009 URL
[21]	Lin Yao. 2019. Genome resequencing dataset based SSR locus analysis and its application in identification and evaluation of peach(Prunus persica)wild resources[M. D. Dissertation]. Nanjing: Nanjing Agricultural University. (in Chinese)
	林瑶. 2019. 基于重测序的桃SSR位点分析及其在野生资源鉴定评价中的应用[硕士论文]. 南京: 南京农业大学.
[22]	Liu K, Muse S V. 2005. PowerMarker:an integrated analysis environment for genetic marker analysis. Bioinformatics(Oxford,England), 21 (9):2128-2129.
[23]	Liu Lili, Li Jianjun, Zheng Xueliang, Chen Jun, Yang Haiying, Liu Chunrong. 2017. Application of DNA molecular markers in peach research. Agricultural Science and Technology Communication,(1):25-28. (in Chinese)
	刘丽丽, 李建辉, 郑雪良, 陈俊, 杨海英, 刘春荣. 2017. DNA分子标记技术在桃研究中的应用. 农业科技通讯,(1):25-28.
[24]	Liu Wei, Li Miao, Li GuiXiang, Dong Xiaomin, Gao Xiaolan, Zhang Anning. 2022. Using SSR fluorescence labeling to construct molecular ID for local peach germplasm resources in Shandong. Shandong Agricultural Sciences, 54 (2):6-13. (in Chinese)
	刘伟, 李淼, 李桂祥, 董晓民, 高晓兰, 张安宁. 2022. 应用SSR荧光标记法构建山东地方桃种质资源分子身份证. 山东农业科学, 54 (2):6-13.
[25]	Lu Caihong. 2025. Discovery of drought-resistant germplasm and identification of drought-regulation candidate genes in Xinjiang wild peach[M. D. Dissertation]. Akesu: Tarim University. (in Chinese)
	卢彩红. 2025. 新疆土桃抗旱优异种质发掘及抗旱调控候选基因鉴定[硕士论文]. 阿克苏: 塔里木大学.
[26]	Lu Zhenhua, Shen Zhijun, Niu Liang, Pan Lei, Cui Guochao, Zeng Wenfang, Wang Zhiqiang. 2020. Molecular marker-assisted identification of yellow/white flesh trait for 122 peach cultivars(lines). Scientia Agricultura Sinica, 53 (14):2929-2940. (in Chinese)
	鲁振华, 沈志军, 牛良, 潘磊, 崔国朝, 曾文芳, 王志强. 2020. 122份栽培桃品种(系)黄白肉性状的分子标记辅助鉴定. 中国农业科学, 53 (14):2929-2940. doi: 10.3864/j.issn.0578-1752.2020.14.016
[27]	Mei Daoyuan, Wang Chen, Wang Zhonghong, Zeng Xiuli. 2022. Research progress in peach breeding in China. Anhui Agricultural Science Bulletin, 28 (2):55-57,64. (in Chinese)
	梅道源, 王晨, 王忠红, 曾秀丽. 2022. 我国桃育种研究进展. 安徽农学通报, 28 (2):55-57,64.
[28]	Peakall R, Smouse P E. 2012. GenAlEx 6.5:genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics(Oxford,England), 28 (19):2537-2539.
[29]	Powell W, Machray G C, Provan J. 1996. Polymorphism revealed by simple sequence repeats. Trends in Plant Science, 1 (7):215-222. doi: 10.1016/1360-1385(96)86898-1 URL
[30]	Pritchard J K, Stephens M J, Donnelly P J. 2000. Inference of population structure using multilocus genotype data. Genetics, 155 (2):945-959. doi: 10.1093/genetics/155.2.945 pmid: 10835412
[31]	Qin Yu, Guo Rongkun, Nong Huilan, Wang Yan, Cui Kai, Dong Ningguang. 2024. Construction of molecular ID for hawthorn germplasm using fluorescent SSR markers. Acta Horticulturae Sinica, 51 (6):1227-1240. (in Chinese)
	秦宇, 郭荣琨, 农惠兰, 王燕, 崔凯, 董宁光. 2024. 利用SSR荧光标记构建山楂种质分子身份证. 园艺学报, 51 (6):1227-1240.
[32]	Qu Yanhua, Li Dongmei, Zhao Lijun, Abbuduwaili · Muminni, Zhu Lixin, Jia Kegong. 2014. Evaluation of acid-alkali-salt tolerance in wild Xinjiang peach(Prunus ferganensis). Journal of China Agricultural University, 19 (3):115-120. (in Chinese)
	曲艳华, 李冬梅, 赵丽君, 阿布都外力 · 木米尼, 朱立新, 贾克功. 2014. 野生新疆桃抗酸碱盐性评价. 中国农业大学学报, 19 (3):115-120.
[33]	Rohlf F J. 1998. NTSYSpc:numerical taxonomy and multivariate analysis system, version 2.02. New York: Applied Biostatistics.
[34]	Savoia M A, Del Faro L, Turco A, Fanelli V, Venerito P, Montemurro C. 2023. Biodiversity evaluation and preservation of Italian stone fruit germplasm(peach and apricot)in southern Italy. Plants, 12 (6):1279. doi: 10.3390/plants12061279 URL
[35]	Sun Yu tong, Liu Deshuai, Feng Mei, Qi Xun, Yao Wenkong. 2024. Research progress on molecular marker-assisted breeding in fruit trees. Jiangsu Journal of Agricultural Sciences, 40 (1):183-192. (in Chinese)
	孙雨桐, 刘德帅, 冯美, 齐迅, 姚文孔. 2024. 果树分子标记辅助育种研究进展. 江苏农业学报, 40 (1):183-192.
[36]	Trifonova A A, Boris K V, Mesyats N V, Tsiupka V A, Smykov A V, Mitrofanova I V. 2021. Genetic diversity of peach cultivars from the collection of the nikita botanical garden based on SSR markers. Plants, 10 (12):2609. doi: 10.3390/plants10122609 URL
[37]	Verde I, Abbott A G, Scalabrin S, Jung S, Shu S, Marroni F, Zhebentyayeva T, Dettori M T, Grimwood J, Cattonaro F, Zuccolo A, Rossini L, Jenkins J, Vendramin E, Meisel L A, Decroocq V, Sosinski B, Prochnik S, Mitros T, Policriti A, Cipriani G, Dondini L, Ficklin S, Goodstein D M, Xuan P, Del Fabbro C, Aramini V, Copetti D, Gonzalez S, Horner DS Falchi R, Lucas S, Mica E, Maldonado J, Lazzari B, Bielenberg D, Pirona R, Miculan M, Barakat A, Testolin R, Stella A, Tartarini S, Tonutti P, Arus P, Orellana A, Wells C, Main D, Vizzotto G, Silva H, Salamini F, Schmutz J, Morgante M, Rokhsar D S. 2013. The high-quality draft genome of peach(Prunus persica)identifies unique patterns of genetic diversity,domestication and genome evolution. Nature Genetics, 45 (5):487-494. doi: 10.1038/ng.2586
[38]	Wang Furong, Tong Zhaoguo, Zhao JianBo, Zhang Zhen, Jiang Quan, Guo Jiying, Jiang Lijie. 2008. AFLP analysis of genetic relationships of wild and local variety germplasm resources in peach. Journal of Fruit Science, 25 (3):305-311. (in Chinese)
	王富荣, 佟兆国, 赵剑波, 章镇, 姜全, 郭继英, 姜立杰. 2008. 桃野生种和地方品种种质资源亲缘关系的AFLP分析. 果树学报, 25 (3):305-311.
[39]	Wang Lin, Ao Dun, Bao Wenquan, Zhang Shuning, Chen Junxing, Li Fengming, Meng Fanqing, Yang Yuying, Bai Yu’e. 2021. Identification of peach cultivars and construction of fingerprint based on SSR markers. Journal of Central South University of Forestry & Technology, 41 (6):131-138. (in Chinese)
	王淋, 敖敦, 包文泉, 张淑宁, 陈俊兴, 李凤鸣, 孟繁庆, 杨钰莹, 白玉娥. 2021. 基于SSR分子标记的桃品种鉴别及指纹图谱构建. 中南林业科技大学学报, 41 (6):131-138.
[40]	Wang Lirong. 2009. Genetic pleiotropy and breeding utilization value of nectarine and flat peach. Journal of Fruit Science, 26 (5):692-698. (in Chinese)
	王力荣. 2009. 油桃、蟠桃的遗传多效性及育种利用价值探讨. 果树学报, 26 (5):692-698.
[41]	Wang Lirong, Zhu Gengrui, Fang Weichao. 2015. Genetic resources and cultivar atlas of peach in China. Beijing: China Agriculture Press. (in Chinese)
	王力荣, 朱更瑞, 方伟超. 2015. 中国桃遗传资源与品种图谱. 北京: 中国农业出版社.
[42]	Wang Lirong. 2021. Historical review and prospect of Chinese peach varieties improvement. Journal of Fruit Science, 38 (12):2178-2195. (in Chinese)
	王力荣. 2021. 中国桃品种改良历史回顾与展望. 果树学报, 38 (12):2178-2195.
[43]	Wang Qingming, Ye Yuyun, Ma Jianwei, Zhang Yinping, Chen Qiyang, Zhang Yong. 2016. Construction of EST-SSR fingerprints for main cultivated peach varieties in Chengdu Plain. Molecular Plant Breeding, 14 (3):638-646. (in Chinese)
	王清明, 叶宇芸, 马建伟, 张银平, 陈其阳, 张勇. 2016. 成都平原桃主栽品种EST-SSR指纹图谱构建. 分子植物育种, 14 (3),638-646.
[44]	Wang Ruixue, Yu Xiaoyan, Wang Haoran, Wu Qichao, Ma Yan, Zang Dekui, Zang Fengqi. 2025. Genetic diversity analysis and fingerprint construction of elite pomegranate varieties based on SSR markers. Journal of Beijing University of Agriculture, 40 (2):34-40. (in Chinese)
	王瑞雪, 于晓燕, 王浩然, 吴其超, 马燕, 臧德奎, 臧凤岐. 2025. 基于SSR标记的石榴良种遗传多样性分析及指纹图谱构建. 北京农学院学报, 40 (2):34-40.
[45]	Xu Baoli. 2006. Genetic diversity and phylogenetic evolution analysis of local peach(Prunus persica L.) germplasm resources in Gansu using SSR markers[M. D. Dissertation]. Lanzhou: Gansu Agricultural University. (in Chinese)
	徐宝利. 2006. 用SSR标记对甘肃地方油桃(Prunus persica L.)种质资源遗传多样性及亲缘演化关系分析[硕士论文]. 兰州: 甘肃农业大学.
[46]	Xu Mengqi, Lu Caihong, Zhu Gengrui, Shao Yajie, Li Yong, Wu Jinlong, Wang Lirong. 2024a. Phenotypic diversity analysis of 133 local peach germplasms in southern Xinjiang. Journal of Fruit Science, 41 (12):2369-2376. (in Chinese)
	徐孟琪, 卢彩红, 朱更瑞, 邵亚杰, 李勇, 吴金龙, 王力荣. 2024a. 南疆133份土桃种质表型多样性分析. 果树学报, 41 (12):2369-2376.
[47]	Xu Mengqi, Li Yong, Wang Lirong. 2024b. Identification technology and application of homonymous peach varieties based on fluorescent SSR markers. Fruit Growers’ Companion,(11):1-6. (in Chinese)
	徐孟琪, 李勇, 王力荣. 2024b. 基于SSR荧光标记法的桃同名异物品种鉴定技术与应用. 果农之友,(11):1-6.
[48]	Xu Peng, Zheng Chunhui, Sun Zhiyong, Yu Lan, Feng Kun. 2018. Experimental study on rapid extraction of genomic DNA by magnetic bead method. Chinese Journal of Bioinformatics, 16 (3):190-195. (in Chinese)
	许朋, 郑春辉, 孙智勇, 余兰, 冯昆. 2018. 磁珠法快速提取基因组DNA的实验研究. 生物信息学, 16 (3):190-195.
[49]	Xu Qingxiu. 1990. Various peaches in Xinjiang. Xinjiang Agricultural Sciences,(1):35. (in Chinese)
	徐庆岫. 1990. 形形色色的新疆桃. 新疆农业科学,(1):35.
[50]	Ye Yuyun, Wang Qingming, MA Jianwei, Zhang Yinping, Chen Qiyang, Tang Haoru, Zhang Yong. 2017. Genetic diversity analysis and comparison of genetic similarity coefficients in peach cultivars based on SSR markers. Journal of South China Agricultural University, 38 (3):39-45. (in Chinese)
	叶宇芸, 王清明, 马建伟, 张银平, 陈其阳, 汤浩茹, 张勇. 2017. 基于SSR标记的桃品种遗传多样性分析及遗传相似系数比较. 华南农业大学学报, 38 (3):39-45.
[51]	Yin Jiwei, Han Beibei, Ma Yingxue, Wu Xingting, Xu Zhenjiang, Jiang Jianfu, Han Ruixi. 2023. Genetic diversity analysis of 154 peach varieties based on SSR molecular markers. Jiangsu Agricultural Sciences, 51 (16):18-26. (in Chinese)
	殷纪伟, 韩贝贝, 马莹雪, 武星廷, 徐振江, 姜建福, 韩瑞玺. 2023. 基于SSR分子标记的154份桃品种遗传多样性分析. 江苏农业科学, 51 (16):18-26.
[52]	Yu Mingliang, Ma Ruijuan, Shen Zhijun, Cai Zhixiang. 2010. Research advances in peach germplasm resources in China. Jiangsu Journal of Agricultural Sciences, 26 (6):1418-1423. (in Chinese)
	俞明亮, 马瑞娟, 沈志军, 蔡志翔. 2010. 中国桃种质资源研究进展. 江苏农业学报, 26 (6):1418-1423.
[53]	Yu Xinyi, Gao Yanan, Yang Yingjie, Liu Jianlong, Ma Chunhui, Cui Zhenhua, Li Dingli. 2025. Construction of molecular ID and genetic diversity analysis of pear rootstocks based on SSR markers. Journal of Qingdao Agricultural University(Natural Science Edition), 42 (2):79-85. (in Chinese)
	于心怡, 高雅楠, 杨英杰, 刘建龙, 马春晖, 崔振华, 李鼎立. 2025. 基于SSR标记的梨砧木分子身份证构建与遗传多样性分析. 青岛农业大学学报(自然科学版), 42 (2):79-85.
[54]	Zhang Junhuan, Zhang Meiling, Yang Li, Jiang Fengchao, Wang Yuzhu, Yu Wenjian, Sun Haoyuan. 2025. Parentage identification of interspecific distant hybridization offspring between apricot and almond using SSR markers. China Fruits,(1):28-34. (in Chinese)
	张俊环, 张美玲, 杨丽, 姜凤超, 王玉柱, 于文剑, 孙浩元. 2025. 杏与扁桃属间远缘杂交后代的SSR标记亲子鉴定. 中国果树,(1):28-34.
[55]	Zhang Yong, Wang Qingming, Ye Yuyun, Tang Haoru. 2019. Development and universality analysis of EST-SSR primers in peach(Prunus persica). Molecular Plant Breeding, 17 (7):2264-2269. (in Chinese)
	张勇, 王清明, 叶宇芸, 汤浩茹. 2019. 桃EST-SSR引物的开发及通用性分析. 分子植物育种, 17 (7):2264-2269.
[56]	Zhang Zhao. 1990. Xinjiang peach germplasm resources. Journal of Plant Genetic Resources,(4):5-6. (in Chinese)
	张钊. 1990. 新疆桃树种质资源. 作物品种资源,(4):5-6.
[57]	Zou Jiawei, Xu Yuqiang, Yang Ming. 2025. Analysis of development strategies for Xinjiang’s peach industry. Xinjiang Agricultural Reclamation Science and Technology, 48 (2):49-51. (in Chinese)
	邹佳伟, 徐宇强, 杨名. 2025. 浅析新疆桃产业的发展思路. 新疆农垦科技, 48 (2):49-51.

来源（种质资源圃） Origin（Germplasm repository）	种质编号 Accession code	类型 Type	来源（农户） Origin（Farmer’s orchard）	种质编号 Accession code	类型 Type
阿克苏地区Akesu	ZA1	油桃Prunus persica var. nectarina	喀什地区Kashi	NK73	毛桃Prunus persica (L.) Batsch
	ZA2	油桃Prunus persica var. nectarina		NK74	毛桃Prunus persica (L.) Batsch
	ZA3	油桃Prunus persica var. nectarina		NK75	油桃Prunus persica var. nectarina
	ZA4	油桃Prunus persica var. nectarina		NK76	油桃Prunus persica var. nectarina
	ZA5	油桃Prunus persica var. nectarina		NK77	油桃Prunus persica var. nectarina
	ZA6	油桃Prunus persica var. nectarina		NK78	毛桃Prunus persica (L.) Batsch
	ZA7	油桃Prunus persica var. nectarina		NK79	毛桃Prunus persica (L.) Batsch
	ZA8	油桃Prunus persica var. nectarina		NK80	毛桃Prunus persica (L.) Batsch
	ZA9	油桃Prunus persica var. nectarina		NK81	毛桃Prunus persica (L.) Batsch
	ZA10	油桃Prunus persica var. nectarina		NK82	毛桃Prunus persica (L.) Batsch
	ZA11	油桃Prunus persica var. nectarina		NK83	毛桃Prunus persica (L.) Batsch
	ZA12	油桃Prunus persica var. nectarina		NK84	毛桃Prunus persica (L.) Batsch
	ZA13	油桃Prunus persica var. nectarina		NK85	油桃Prunus persica var. nectarina
	ZA14	油桃Prunus persica var. nectarina		NK86	油桃Prunus persica var. nectarina
	ZA15	油桃Prunus persica var. nectarina		NK87	油桃Prunus persica var. nectarina
	ZA16	油桃Prunus persica var. nectarina		NK88	油桃Prunus persica var. nectarina
	ZA17	毛桃Prunus persica (L.) Batsch		NK89	毛桃Prunus persica (L.) Batsch
	ZA18	毛桃Prunus persica (L.) Batsch		NK90	毛桃Prunus persica (L.) Batsch
	ZA19	毛桃Prunus persica (L.) Batsch		NK91	毛桃Prunus persica (L.) Batsch
	ZA20	毛桃Prunus persica (L.) Batsch		NK92	毛桃Prunus persica (L.) Batsch
	ZA21	毛桃Prunus persica (L.) Batsch		NK93	毛桃Prunus persica (L.) Batsch
	ZA22	毛桃Prunus persica (L.) Batsch		NK94	毛桃Prunus persica (L.) Batsch
	ZA23	毛桃Prunus persica (L.) Batsch		NK95	毛桃Prunus persica (L.) Batsch
	ZA24	毛桃Prunus persica (L.) Batsch		NK96	毛桃Prunus persica (L.) Batsch
	ZA25	毛桃Prunus persica (L.) Batsch		NK97	毛桃Prunus persica (L.) Batsch
	ZA26	毛桃Prunus persica (L.) Batsch		NK98	毛桃Prunus persica (L.) Batsch
喀什地区Kashi	ZK27	油桃Prunus persica var. nectarina		NK99	毛桃Prunus persica (L.) Batsch
	ZK28	油桃Prunus persica var. nectarina		NK100	毛桃Prunus persica (L.) Batsch
	ZK29	油桃Prunus persica var. nectarina		NK101	毛桃Prunus persica (L.) Batsch
	ZK30	油桃Prunus persica var. nectarina		NK102	油桃Prunus persica var. nectarina
	ZK31	油桃Prunus persica var. nectarina		NK103	毛桃Prunus persica (L.) Batsch
	ZK32	油桃Prunus persica var. nectarina		NK104	毛桃Prunus persica (L.) Batsch
	ZK33	油桃Prunus persica var. nectarina		NK105	毛桃Prunus persica (L.) Batsch
	ZK34	毛蟠桃Prunus persica var. platycarpa		NK106	毛桃Prunus persica (L.) Batsch
	ZK35	毛桃Prunus persica (L.) Batsch		NK107	毛桃Prunus persica (L.) Batsch
	ZK36	毛桃Prunus persica (L.) Batsch		NK108	毛桃Prunus persica (L.) Batsch
	ZK37	毛桃Prunus persica (L.) Batsch		NK109	毛桃Prunus persica (L.) Batsch
	ZK38	毛桃Prunus persica (L.) Batsch		NK110	毛桃Prunus persica (L.) Batsch
喀什地区Kashi	ZK39	毛桃Prunus persica (L.) Batsch	喀什地区Kashi	NK111	毛桃Prunus persica (L.) Batsch
	ZK40	毛桃Prunus persica (L.) Batsch		NK112	毛桃Prunus persica (L.) Batsch
	ZK41	毛桃Prunus persica (L.) Batsch		NK113	毛桃Prunus persica (L.) Batsch
	ZK42	毛桃Prunus persica (L.) Batsch		NK114	油桃Prunus persica var. nectarina
	ZK43	毛桃Prunus persica (L.) Batsch		NK115	油桃Prunus persica var. nectarina
	ZK44	毛桃Prunus persica (L.) Batsch		NK116	毛桃Prunus persica (L.) Batsch
	ZK45	毛桃Prunus persica (L.) Batsch		NK117	毛桃Prunus persica (L.) Batsch
	ZK46	毛桃Prunus persica (L.) Batsch		NK118	毛桃Prunus persica (L.) Batsch
	ZK47	毛桃Prunus persica (L.) Batsch		NK119	毛桃Prunus persica (L.) Batsch
	ZK48	毛桃Prunus persica (L.) Batsch		NK120	毛桃Prunus persica (L.) Batsch
	ZK49	毛桃Prunus persica (L.) Batsch	和田地区Hotan	NH121	油桃Prunus persica var. nectarina
	ZK50	毛桃Prunus persica (L.) Batsch		NH122	油桃Prunus persica var. nectarina
	ZK51	毛桃Prunus persica (L.) Batsch		NH123	毛桃Prunus persica (L.) Batsch
	ZK52	毛桃Prunus persica (L.) Batsch		NH124	毛桃Prunus persica (L.) Batsch
	ZK53	毛桃Prunus persica (L.) Batsch		NH125	毛桃Prunus persica (L.) Batsch
	ZK54	毛桃Prunus persica (L.) Batsch		NH126	毛桃Prunus persica (L.) Batsch
和田地区Hotan	ZH55	油桃Prunus persica var. nectarina		NH127	毛桃Prunus persica (L.) Batsch
	ZH56	油桃Prunus persica var. nectarina		NH128	毛桃Prunus persica (L.) Batsch
	ZH57	油蟠桃Flat nectarine		NH129	毛桃Prunus persica (L.) Batsch
	ZH58	毛桃Prunus persica (L.) Batsch		NH130	毛桃Prunus persica (L.) Batsch
	ZH59	毛桃Prunus persica (L.) Batsch		NH131	毛桃Prunus persica (L.) Batsch
	ZH60	毛桃Prunus persica (L.) Batsch		NH132	毛桃Prunus persica (L.) Batsch
	ZH61	毛桃Prunus persica (L.) Batsch		NH133	毛桃Prunus persica (L.) Batsch
	ZH62	毛桃Prunus persica (L.) Batsch	克孜勒苏柯尔克孜自治州 Kizilsu Kirghiz	NK134	油桃Prunus persica var. nectarina
	ZH63	毛桃Prunus persica (L.) Batsch		NK135	油桃Prunus persica var. nectarina
	ZH64	毛桃Prunus persica (L.) Batsch		NK136	油桃Prunus persica var. nectarina
	ZH65	毛桃Prunus persica (L.) Batsch		NK137	毛桃Prunus persica (L.) Batsch
	ZH66	毛桃Prunus persica (L.) Batsch		NK138	毛桃Prunus persica (L.) Batsch
	ZH67	毛桃Prunus persica (L.) Batsch		NK139	毛桃Prunus persica (L.) Batsch
	ZH68	毛桃Prunus persica (L.) Batsch		NK140	毛桃Prunus persica (L.) Batsch
	ZH69	毛桃Prunus persica (L.) Batsch		NK141	毛桃Prunus persica (L.) Batsch
	ZH70	毛桃Prunus persica (L.) Batsch		NK142	毛桃Prunus persica (L.) Batsch
	ZH71	毛桃Prunus persica (L.) Batsch		NK143	毛桃Prunus persica (L.) Batsch
	ZH72	毛桃Prunus persica (L.) Batsch		NK144	毛桃Prunus persica (L.) Batsch
				NK145	毛桃Prunus persica (L.) Batsch
				NK146	毛桃Prunus persica (L.) Batsch
				NK147	毛桃Prunus persica (L.) Batsch
				NK148	毛桃Prunus persica (L.) Batsch
				NK149	毛桃Prunus persica (L.) Batsch
				NK150	毛桃Prunus persica (L.) Batsch
				NK151	毛桃Prunus persica (L.) Batsch
				NK152	毛桃Prunus persica (L.) Batsch
				NK153	毛桃Prunus persica (L.) Batsch

来源（种质资源圃） Origin（Germplasm repository）	种质编号 Accession code	类型 Type	来源（农户） Origin（Farmer’s orchard）	种质编号 Accession code	类型 Type
阿克苏地区Akesu	ZA1	油桃Prunus persica var. nectarina	喀什地区Kashi	NK73	毛桃Prunus persica (L.) Batsch
	ZA2	油桃Prunus persica var. nectarina		NK74	毛桃Prunus persica (L.) Batsch
	ZA3	油桃Prunus persica var. nectarina		NK75	油桃Prunus persica var. nectarina
	ZA4	油桃Prunus persica var. nectarina		NK76	油桃Prunus persica var. nectarina
	ZA5	油桃Prunus persica var. nectarina		NK77	油桃Prunus persica var. nectarina
	ZA6	油桃Prunus persica var. nectarina		NK78	毛桃Prunus persica (L.) Batsch
	ZA7	油桃Prunus persica var. nectarina		NK79	毛桃Prunus persica (L.) Batsch
	ZA8	油桃Prunus persica var. nectarina		NK80	毛桃Prunus persica (L.) Batsch
	ZA9	油桃Prunus persica var. nectarina		NK81	毛桃Prunus persica (L.) Batsch
	ZA10	油桃Prunus persica var. nectarina		NK82	毛桃Prunus persica (L.) Batsch
	ZA11	油桃Prunus persica var. nectarina		NK83	毛桃Prunus persica (L.) Batsch
	ZA12	油桃Prunus persica var. nectarina		NK84	毛桃Prunus persica (L.) Batsch
	ZA13	油桃Prunus persica var. nectarina		NK85	油桃Prunus persica var. nectarina
	ZA14	油桃Prunus persica var. nectarina		NK86	油桃Prunus persica var. nectarina
	ZA15	油桃Prunus persica var. nectarina		NK87	油桃Prunus persica var. nectarina
	ZA16	油桃Prunus persica var. nectarina		NK88	油桃Prunus persica var. nectarina
	ZA17	毛桃Prunus persica (L.) Batsch		NK89	毛桃Prunus persica (L.) Batsch
	ZA18	毛桃Prunus persica (L.) Batsch		NK90	毛桃Prunus persica (L.) Batsch
	ZA19	毛桃Prunus persica (L.) Batsch		NK91	毛桃Prunus persica (L.) Batsch
	ZA20	毛桃Prunus persica (L.) Batsch		NK92	毛桃Prunus persica (L.) Batsch
	ZA21	毛桃Prunus persica (L.) Batsch		NK93	毛桃Prunus persica (L.) Batsch
	ZA22	毛桃Prunus persica (L.) Batsch		NK94	毛桃Prunus persica (L.) Batsch
	ZA23	毛桃Prunus persica (L.) Batsch		NK95	毛桃Prunus persica (L.) Batsch
	ZA24	毛桃Prunus persica (L.) Batsch		NK96	毛桃Prunus persica (L.) Batsch
	ZA25	毛桃Prunus persica (L.) Batsch		NK97	毛桃Prunus persica (L.) Batsch
	ZA26	毛桃Prunus persica (L.) Batsch		NK98	毛桃Prunus persica (L.) Batsch
喀什地区Kashi	ZK27	油桃Prunus persica var. nectarina		NK99	毛桃Prunus persica (L.) Batsch
	ZK28	油桃Prunus persica var. nectarina		NK100	毛桃Prunus persica (L.) Batsch
	ZK29	油桃Prunus persica var. nectarina		NK101	毛桃Prunus persica (L.) Batsch
	ZK30	油桃Prunus persica var. nectarina		NK102	油桃Prunus persica var. nectarina
	ZK31	油桃Prunus persica var. nectarina		NK103	毛桃Prunus persica (L.) Batsch
	ZK32	油桃Prunus persica var. nectarina		NK104	毛桃Prunus persica (L.) Batsch
	ZK33	油桃Prunus persica var. nectarina		NK105	毛桃Prunus persica (L.) Batsch
	ZK34	毛蟠桃Prunus persica var. platycarpa		NK106	毛桃Prunus persica (L.) Batsch
	ZK35	毛桃Prunus persica (L.) Batsch		NK107	毛桃Prunus persica (L.) Batsch
	ZK36	毛桃Prunus persica (L.) Batsch		NK108	毛桃Prunus persica (L.) Batsch
	ZK37	毛桃Prunus persica (L.) Batsch		NK109	毛桃Prunus persica (L.) Batsch
	ZK38	毛桃Prunus persica (L.) Batsch		NK110	毛桃Prunus persica (L.) Batsch
喀什地区Kashi	ZK39	毛桃Prunus persica (L.) Batsch	喀什地区Kashi	NK111	毛桃Prunus persica (L.) Batsch
	ZK40	毛桃Prunus persica (L.) Batsch		NK112	毛桃Prunus persica (L.) Batsch
	ZK41	毛桃Prunus persica (L.) Batsch		NK113	毛桃Prunus persica (L.) Batsch
	ZK42	毛桃Prunus persica (L.) Batsch		NK114	油桃Prunus persica var. nectarina
	ZK43	毛桃Prunus persica (L.) Batsch		NK115	油桃Prunus persica var. nectarina
	ZK44	毛桃Prunus persica (L.) Batsch		NK116	毛桃Prunus persica (L.) Batsch
	ZK45	毛桃Prunus persica (L.) Batsch		NK117	毛桃Prunus persica (L.) Batsch
	ZK46	毛桃Prunus persica (L.) Batsch		NK118	毛桃Prunus persica (L.) Batsch
	ZK47	毛桃Prunus persica (L.) Batsch		NK119	毛桃Prunus persica (L.) Batsch
	ZK48	毛桃Prunus persica (L.) Batsch		NK120	毛桃Prunus persica (L.) Batsch
	ZK49	毛桃Prunus persica (L.) Batsch	和田地区Hotan	NH121	油桃Prunus persica var. nectarina
	ZK50	毛桃Prunus persica (L.) Batsch		NH122	油桃Prunus persica var. nectarina
	ZK51	毛桃Prunus persica (L.) Batsch		NH123	毛桃Prunus persica (L.) Batsch
	ZK52	毛桃Prunus persica (L.) Batsch		NH124	毛桃Prunus persica (L.) Batsch
	ZK53	毛桃Prunus persica (L.) Batsch		NH125	毛桃Prunus persica (L.) Batsch
	ZK54	毛桃Prunus persica (L.) Batsch		NH126	毛桃Prunus persica (L.) Batsch
和田地区Hotan	ZH55	油桃Prunus persica var. nectarina		NH127	毛桃Prunus persica (L.) Batsch
	ZH56	油桃Prunus persica var. nectarina		NH128	毛桃Prunus persica (L.) Batsch
	ZH57	油蟠桃Flat nectarine		NH129	毛桃Prunus persica (L.) Batsch
	ZH58	毛桃Prunus persica (L.) Batsch		NH130	毛桃Prunus persica (L.) Batsch
	ZH59	毛桃Prunus persica (L.) Batsch		NH131	毛桃Prunus persica (L.) Batsch
	ZH60	毛桃Prunus persica (L.) Batsch		NH132	毛桃Prunus persica (L.) Batsch
	ZH61	毛桃Prunus persica (L.) Batsch		NH133	毛桃Prunus persica (L.) Batsch
	ZH62	毛桃Prunus persica (L.) Batsch	克孜勒苏柯尔克孜自治州 Kizilsu Kirghiz	NK134	油桃Prunus persica var. nectarina
	ZH63	毛桃Prunus persica (L.) Batsch		NK135	油桃Prunus persica var. nectarina
	ZH64	毛桃Prunus persica (L.) Batsch		NK136	油桃Prunus persica var. nectarina
	ZH65	毛桃Prunus persica (L.) Batsch		NK137	毛桃Prunus persica (L.) Batsch
	ZH66	毛桃Prunus persica (L.) Batsch		NK138	毛桃Prunus persica (L.) Batsch
	ZH67	毛桃Prunus persica (L.) Batsch		NK139	毛桃Prunus persica (L.) Batsch
	ZH68	毛桃Prunus persica (L.) Batsch		NK140	毛桃Prunus persica (L.) Batsch
	ZH69	毛桃Prunus persica (L.) Batsch		NK141	毛桃Prunus persica (L.) Batsch
	ZH70	毛桃Prunus persica (L.) Batsch		NK142	毛桃Prunus persica (L.) Batsch
	ZH71	毛桃Prunus persica (L.) Batsch		NK143	毛桃Prunus persica (L.) Batsch
	ZH72	毛桃Prunus persica (L.) Batsch		NK144	毛桃Prunus persica (L.) Batsch
				NK145	毛桃Prunus persica (L.) Batsch
				NK146	毛桃Prunus persica (L.) Batsch
				NK147	毛桃Prunus persica (L.) Batsch
				NK148	毛桃Prunus persica (L.) Batsch
				NK149	毛桃Prunus persica (L.) Batsch
				NK150	毛桃Prunus persica (L.) Batsch
				NK151	毛桃Prunus persica (L.) Batsch
				NK152	毛桃Prunus persica (L.) Batsch
				NK153	毛桃Prunus persica (L.) Batsch

连锁群 LG	引物名称 Marker	引物序列（5′-3′） Primer Sequence	重复序列 Motif	扩增位点 Locus	产物大小/bp Size	参考文献 Reference
1	SSR152	F：GTTCTCGACTCCCATATCCAA	(TC)28	Pp01:1400332- 1400582	250	关利平等,2021
	SSR152	R：CTCCAAAGTACAGAGCCTATCG	(TC)28	Pp01:1400332- 1400582	250	关利平等,2021
	Pp1-4	F：CCAACAAAACCAAACTCAACCC	(AG)12	Pp01:13906635- 13906795	160	林瑶,2019
	Pp1-4	R：ACAACAACCTGGCTCTCTTCC	(AG)12	Pp01:13906635- 13906795	160	林瑶,2019
2	SSR96	F：AACCTCAATCATTCTTTACACAAGC	(GA)22	Pp02:29445050- 29445196	146	关利平等,2021
	SSR96	R：CTGCTTAAGGAGGAACCTCAAAT	(GA)22	Pp02:29445050- 29445196	146	关利平等,2021
	Pp2-3	F：CTCAGCTTCTCTTCCCTCTTCC	(TC)20	Pp02:7762934- 7763083	149	林瑶,2019
	Pp2-3	R：TGCGCTGCATATACAAGTGAAG	(TC)20	Pp02:7762934- 7763083	149	林瑶,2019
3	SSR184	F：TGAATGTTCTTCCTGCTCCTG	(GA)33	Pp03:4210394- 4210684	290	关利平等,2021
	SSR184	R：ATGAACATGAACCAGTCAAGGA	(GA)33	Pp03:4210394- 4210684	290	关利平等,2021
	Pp3-4	F：AGCTGAAACTTAGGGGAAGGC	(GA)16	Pp03:22922391- 22922517	126	林瑶,2019
	Pp3-4	R：GGGATTGAAAACAACCCAACCC	(GA)16	Pp03:22922391- 22922517	126	林瑶,2019
4	SSR73	F：TTGCTGCTGAAAAATAATGAACA	(CAA)6	Pp04:25147615- 25147775	160	关利平等,2021
	SSR73	R：GGGTGGCCTGTTGAGAATATAA	(CAA)6	Pp04:25147615- 25147775	160	关利平等,2021
	Pp4-5	F：TGCATTCTGGTCGTGACTTGG	(TC)6	Pp04:3657853- 3657998	145	林瑶,2019
	Pp4-5	R：CCACCATGATTTGAGGTGCTG	(TC)6	Pp04:3657853- 3657998	145	林瑶,2019
5	BPPCT026	F：ATACCTTTGCCACTTGCG	(AG)19	Pp05:4389436- 4389578	142	Dirlewanger et al.,2002
	BPPCT026	R：TGAGTTGGAAGAAAACGTAACA	(AG)19	Pp05:4389436- 4389578	142	Dirlewanger et al.,2002
	Pp5-2	F：CAGGACGTTTCTGTTAGCCTG	(GA)18	Pp05:16696022- 16696164	142	林瑶,2019
	Pp5-2	R：TGTGGAAGGCTATAGAGTGGT	(GA)18	Pp05:16696022- 16696164	142	林瑶,2019
6	AJ826975	F：TGTTGCTTTTTCAAGTTGCG	(TC)17	Pp06:729646- 729814	168	叶宇芸等,2017
	AJ826975	R：AAGGTTTACATCAGTGCCCG	(TC)17	Pp06:729646- 729814	168	叶宇芸等,2017
	BU041198	F：TTGCCAGTTCATCATTGTTTG	(GA)19	Pp06:106385- 106587	202	叶宇芸等,2017
	BU041198	R：ACCATTATGCCTTGGTCAGG	(GA)19	Pp06:106385- 106587	202	叶宇芸等,2017
7	CPPCT022	F：CAATTAGCTAGAGAGAATTATTG	(AG)27	Pp07:10612186- 10612485	299	Aranzana et al.,2002
	CPPCT022	R：GACAAGAAGCAAGTAGTTTG	(AG)27	Pp07:10612186- 10612485	299	Aranzana et al.,2002
	Pp7-1	F：ACCCTTAAAAAGTGGCTGCCT	(TC)14	Pp07:15278492- 15278616	124	林瑶,2019
	Pp7-1	R：ATGCATAACAGAGAAACCCTCG	(TC)14	Pp07:15278492- 15278616	124	林瑶,2019
8	SSR93	F：AACTGCCTTAGCTTAGACTGGCT	(AG)10	Pp08:17345674- 17345834	160	关利平等,2021
	SSR93	R：AAGACGAGAAACCACCTTGAATC	(AG)10	Pp08:17345674- 17345834	160	关利平等,2021
	Pp8-5	F：TGGACCGTTTGGTCTTATGCT	(TC)16	Pp08:20184536- 20184669	133	林瑶,2019
	Pp8-5	R：GGCACCGAATTCCACTATTTCG	(TC)16	Pp08:20184536- 20184669	133	林瑶,2019

连锁群 LG	引物名称 Marker	引物序列（5′-3′） Primer Sequence	重复序列 Motif	扩增位点 Locus	产物大小/bp Size	参考文献 Reference
1	SSR152	F：GTTCTCGACTCCCATATCCAA	(TC)28	Pp01:1400332- 1400582	250	关利平等,2021
	SSR152	R：CTCCAAAGTACAGAGCCTATCG	(TC)28	Pp01:1400332- 1400582	250	关利平等,2021
	Pp1-4	F：CCAACAAAACCAAACTCAACCC	(AG)12	Pp01:13906635- 13906795	160	林瑶,2019
	Pp1-4	R：ACAACAACCTGGCTCTCTTCC	(AG)12	Pp01:13906635- 13906795	160	林瑶,2019
2	SSR96	F：AACCTCAATCATTCTTTACACAAGC	(GA)22	Pp02:29445050- 29445196	146	关利平等,2021
	SSR96	R：CTGCTTAAGGAGGAACCTCAAAT	(GA)22	Pp02:29445050- 29445196	146	关利平等,2021
	Pp2-3	F：CTCAGCTTCTCTTCCCTCTTCC	(TC)20	Pp02:7762934- 7763083	149	林瑶,2019
	Pp2-3	R：TGCGCTGCATATACAAGTGAAG	(TC)20	Pp02:7762934- 7763083	149	林瑶,2019
3	SSR184	F：TGAATGTTCTTCCTGCTCCTG	(GA)33	Pp03:4210394- 4210684	290	关利平等,2021
	SSR184	R：ATGAACATGAACCAGTCAAGGA	(GA)33	Pp03:4210394- 4210684	290	关利平等,2021
	Pp3-4	F：AGCTGAAACTTAGGGGAAGGC	(GA)16	Pp03:22922391- 22922517	126	林瑶,2019
	Pp3-4	R：GGGATTGAAAACAACCCAACCC	(GA)16	Pp03:22922391- 22922517	126	林瑶,2019
4	SSR73	F：TTGCTGCTGAAAAATAATGAACA	(CAA)6	Pp04:25147615- 25147775	160	关利平等,2021
	SSR73	R：GGGTGGCCTGTTGAGAATATAA	(CAA)6	Pp04:25147615- 25147775	160	关利平等,2021
	Pp4-5	F：TGCATTCTGGTCGTGACTTGG	(TC)6	Pp04:3657853- 3657998	145	林瑶,2019
	Pp4-5	R：CCACCATGATTTGAGGTGCTG	(TC)6	Pp04:3657853- 3657998	145	林瑶,2019
5	BPPCT026	F：ATACCTTTGCCACTTGCG	(AG)19	Pp05:4389436- 4389578	142	Dirlewanger et al.,2002
	BPPCT026	R：TGAGTTGGAAGAAAACGTAACA	(AG)19	Pp05:4389436- 4389578	142	Dirlewanger et al.,2002
	Pp5-2	F：CAGGACGTTTCTGTTAGCCTG	(GA)18	Pp05:16696022- 16696164	142	林瑶,2019
	Pp5-2	R：TGTGGAAGGCTATAGAGTGGT	(GA)18	Pp05:16696022- 16696164	142	林瑶,2019
6	AJ826975	F：TGTTGCTTTTTCAAGTTGCG	(TC)17	Pp06:729646- 729814	168	叶宇芸等,2017
	AJ826975	R：AAGGTTTACATCAGTGCCCG	(TC)17	Pp06:729646- 729814	168	叶宇芸等,2017
	BU041198	F：TTGCCAGTTCATCATTGTTTG	(GA)19	Pp06:106385- 106587	202	叶宇芸等,2017
	BU041198	R：ACCATTATGCCTTGGTCAGG	(GA)19	Pp06:106385- 106587	202	叶宇芸等,2017
7	CPPCT022	F：CAATTAGCTAGAGAGAATTATTG	(AG)27	Pp07:10612186- 10612485	299	Aranzana et al.,2002
	CPPCT022	R：GACAAGAAGCAAGTAGTTTG	(AG)27	Pp07:10612186- 10612485	299	Aranzana et al.,2002
	Pp7-1	F：ACCCTTAAAAAGTGGCTGCCT	(TC)14	Pp07:15278492- 15278616	124	林瑶,2019
	Pp7-1	R：ATGCATAACAGAGAAACCCTCG	(TC)14	Pp07:15278492- 15278616	124	林瑶,2019
8	SSR93	F：AACTGCCTTAGCTTAGACTGGCT	(AG)10	Pp08:17345674- 17345834	160	关利平等,2021
	SSR93	R：AAGACGAGAAACCACCTTGAATC	(AG)10	Pp08:17345674- 17345834	160	关利平等,2021
	Pp8-5	F：TGGACCGTTTGGTCTTATGCT	(TC)16	Pp08:20184536- 20184669	133	林瑶,2019
	Pp8-5	R：GGCACCGAATTCCACTATTTCG	(TC)16	Pp08:20184536- 20184669	133	林瑶,2019

引物名称 Marker	等位基因数 N_a	有效等位基因数 N_e	香农信息指数I	观测杂合度 H_o	期望杂合度 H_e	多态信息含量 PIC
SSR152	8	3.19	1.37	0.20	0.69	0.64
Pp1-4	10	4.64	1.70	0.26	0.78	0.75
SSR96	12	4.70	1.78	0.24	0.79	0.76
Pp2-3	7	1.97	1.04	0.20	0.49	0.46
SSR184	7	2.62	1.16	0.17	0.62	0.55
Pp3-4	9	3.49	1.59	0.23	0.71	0.69
SSR73	4	2.23	1.05	0.18	0.55	0.51
Pp4-5	6	1.37	0.59	0.12	0.27	0.26
BPPCT026	5	2.56	1.09	0.11	0.61	0.54
Pp5-2	4	1.50	0.56	0.08	0.33	0.29
AJ826975	4	1.10	0.23	0.05	0.09	0.09
BU041198	6	1.43	0.65	0.12	0.30	0.28
CPPCT022	14	3.34	1.71	0.13	0.70	0.68
Pp7-1	8	2.14	1.07	0.19	0.53	0.48
SSR93	5	2.02	0.95	0.14	0.50	0.45
Pp8-5	12	2.83	1.41	0.21	0.65	0.61
均值 Mean	7.56	2.57	1.12	0.17	0.54	0.50

基于SSR分子标记的新疆桃地方品种遗传多样性分析及DNA指纹图谱的构建

Genetic Diversity Analysis and DNA Fingerprinting Development of Prunus ferganensis Landrace Germplasm Based on SSR

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 57

相关文章 15

编辑推荐

Metrics

本文评价

访问总数:　当日访问总数:　当前在线人数:

类群 Group	类型 Type		分布地区 Distribution region				资源数量 Number of accessions
类群 Group	毛桃 Hairy peach	油桃 Nectarine	阿克苏 Akesu	喀什 Kashi	和田 Hotan	克州 Kizilsu	资源数量 Number of accessions
类群 Ⅰ	4	2	2	2	2	0	6
类群 Ⅱ	5	2	1	2	4	0	7
类群 Ⅲ	12	1	2	6	3	2	13
类群 Ⅳ	1	4	0	2	3	0	5
类群 Ⅴ	3	10	2	5	3	3	13
类群 Ⅵ	7	0	0	7	0	0	7
类群 Ⅶ	0	10	7	3	0	0	10
类群 Ⅷ	20	8	12	14	0	2	28
类群 Ⅸ	60	4	0	35	16	13	64
总数Total	112	41	26	76	31	20	153

连锁群 LG	引物 Primer	编码 Code
连锁群 LG	引物 Primer	1	2	3	4	5	6	7	8	9	A	B	C	D	E
1	Pp1-4	146	156	158	159	161	163	165	167	169	171
2	SSR96	117	123	125	129	131	133	139	142	144	146	148	150
3	Pp3-4	120	124	126	128	132	134	136	138	140
4	SSR73	158	170	184	193
5	BPPCT026	132	140	142	146	148
6	BU041198	198	200	202	204	206	209
7	CPPCT022	252	271	273	275	280	282	284	286	290	291	293	295	297	299
8	Pp8-5	124	132	134	136	138	150	152	153	159	161	163	169

[1]	王莹莹, 王孝娣, 杨兴旺, 刘万春. 早熟桃新品种‘中农早春’[J]. 园艺学报, 2026, 53(S1): 31-32.
[2]	陈昌文, 曹珂, 方伟超, 朱更瑞, 王新卫, 李勇, 吴金龙, 王力荣. 中熟黄肉桃新品种‘中桃金美’[J]. 园艺学报, 2026, 53(S1): 33-34.
[3]	杨兴旺, 王海波, 王莹莹, 张望舒, 史祥宾, 刘万春, 王孝娣. 晚熟抗寒桃新品种‘中寒秋阳’[J]. 园艺学报, 2026, 53(S1): 35-36.
[4]	时梦, 王海波, 刘立常, 刘志伍, 王莹莹, 杨兴旺, 王孝娣. 极晚熟桃新品种‘中农晚蜜’[J]. 园艺学报, 2026, 53(S1): 37-38.
[5]	于坤淼, 李鹏, 王莹莹, 杨兴旺, 张艺灿, 刘畅, 刘万春, 王孝娣. 抗寒桃新品种‘中寒5号’[J]. 园艺学报, 2026, 53(S1): 39-40.
[6]	于坤淼, 李鹏, 王莹莹, 杨兴旺, 刘培培, 刘畅, 刘万春, 王孝娣. 抗寒桃新品种‘中寒6号’[J]. 园艺学报, 2026, 53(S1): 41-42.
[7]	王富荣, 艾小艳, 王会良, 刘勇, 朱炜, 张杨, 甘志猛, 顾霞, 刘模发, 诸小敏, 黎刚, 何华平, 龚林忠. 红肉油桃新品种‘楚红3号’[J]. 园艺学报, 2026, 53(S1): 43-44.
[8]	许盼云, 徐叶挺, 王秀娟, 张洋军, 葛立鑫, 木巴热克·阿尤普. 扁桃新品种‘疆扁丰’[J]. 园艺学报, 2026, 53(S1): 45-46.
[9]	韩飞, 李大卫, 张琼, 刘小莉, 田华, 钟彩虹. 特早花猕猴桃雄性新品种‘磨山雄7号’[J]. 园艺学报, 2026, 53(S1): 57-58.
[10]	李坤, 杨林, 杨玉皎, 许智萍, 陈大明, 刘元伟, 王先位, 张斌艳. 美味猕猴桃新品种‘邓猕1号’[J]. 园艺学报, 2026, 53(S1): 59-60.
[11]	刘清华, 王策, 蒋迎春, 袁炎良, 陈奇. 黄肉猕猴桃新品种‘先沃七号’[J]. 园艺学报, 2026, 53(S1): 61-62.
[12]	罗轩, 杨晓斌, 高磊, 陈庆红, 汪志, 叶丽霞, 黄穷, 张蕾. 红肉软枣猕猴桃新品种‘金红豆’[J]. 园艺学报, 2026, 53(S1): 63-64.
[13]	张建瑛, 田新华, 殷东生. 软枣猕猴桃新品种‘绿福3号’[J]. 园艺学报, 2026, 53(S1): 65-66.
[14]	张庆朝, 冯博杰, 陶利婷, 徐晓薇, 陈瑞, 张立华. 猕猴桃新品种‘泰玉’[J]. 园艺学报, 2026, 53(S1): 67-68.
[15]	黄闽敏, 宁万军, 张毅, 王宝庆, 马合木提·阿不来提, 阿迪来·买买提, 张强. 核桃新品种‘墨宝’[J]. 园艺学报, 2026, 53(S1): 69-70.

基于SSR分子标记的新疆桃地方品种遗传多样性分析及DNA指纹图谱的构建

Genetic Diversity Analysis and DNA Fingerprinting Development of Prunus ferganensis Landrace Germplasm Based on SSR

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 57

相关文章 15

编辑推荐

Metrics

本文评价

访问总数: 当日访问总数: 当前在线人数:

访问总数:　当日访问总数:　当前在线人数: