甘蓝黑斑病菌鉴定、生物学特性分析及抗性种质资源筛选

doi:10.16420/j.issn.0513-353x.2025-0509

园艺学报 ›› 2026, Vol. 53 ›› Issue (5): 1502-1520.doi: 10.16420/j.issn.0513-353x.2025-0509

甘蓝黑斑病菌鉴定、生物学特性分析及抗性种质资源筛选

王彩红¹, 张扬勇², 李森¹, 张朝文¹, 孔小平³, 吕红豪², 王勇², 杨丽梅², 季家磊²^,^*(), 庄木²^,^*()

¹ 山西农业大学园艺学院，山西太谷030801
² 中国农业科学院蔬菜花卉研究所，蔬菜生物育种全国重点实验室，北京 100081
³ 西宁市蔬菜技术服务中心，西宁 810016

收稿日期:2025-09-28 修回日期:2025-12-19 出版日期:2026-05-26 发布日期:2026-05-26
通讯作者:
^* E-mail：jijialei@caas.cn，
zhuangmu@caas.cn
基金资助:
国家重点研发计划项目(2023YFD2001201); 青海省重大科技专项(2024-BJ-A5); 川渝科技创新合作计划项目(CSTB2024TIADCYKJCXX0022); 重庆市人民政府与中国农业科学院战略合作资助项目

Identification of Black Spot Pathogen in Cabbage，Analysis of Biological Characteristics，and Screening of Resistant Germplasm Resources

WANG Caihong¹, ZHANG Yangyong², LI Sen¹, ZHANG Chaowen¹, KONG Xiaoping³, LÜ Honghao², WANG Yong², YANG Limei², JI Jialei²^,^*(), ZHUANG Mu²^,^*()

¹ College of Horticulture， Shanxi Agricultural University，Taigu， Shanxi 030801， China
² State Key Laboratory of Vegetable Biobreeding， Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences， Beijing 100081， China
³ Xining Vegetable Technology Service Center， Xining 810016， China

Received:2025-09-28 Revised:2025-12-19 Published:2026-05-26 Online:2026-05-26
Contact:
^* E-mail：jijialei@caas.cn，
zhuangmu@caas.cn

摘要/Abstract

摘要：

黑斑病是制约甘蓝生产的重要病害之一。从北京、重庆、湖北、四川、内蒙古5个甘蓝主产区采集黑斑病样本多份，经分离、纯化获得30株黑斑病病原菌，通过观察明确了黑斑病病原菌的菌落、孢子及孢子链形态特征。通过菌块接种法对上述病原菌进行致病性鉴定，确定从北京筛选出的BJA1菌株致病性最强。通过克隆BJA1菌株的ITS、TEF1、GAPDH基因，并进行系统发育分析，明确BJA1是芸薹生链格孢（Alternaria brassicicola）的典型菌株。利用该菌株建立了一套高效、稳定的甘蓝黑斑病抗性离体接种鉴定体系，确定适宜接种的菌液浓度为1 × 10⁵ spores · mL^-1，适宜接种的苗龄为四叶期。应用该体系对100份甘蓝种质进行黑斑病抗性评价，从中筛选出24份中抗材料，其中1份抗病材料病情指数25.93。

关键词: 甘蓝, 黑斑病, 芸薹生链格孢, 抗性鉴定, 抗源筛选

Abstract:

Black spot disease is one of the major constraints in cabbage production. Multiple black spot disease samples were collected from five major cabbage production regions Beijing，Chongqing，Hubei，Sichuan，and Inner Mongolia. After isolation and purification，30 strains of the pathogen were obtained. Morphological characteristics of the colonies，conidia，and conidial chains of the pathogen were observed and described. Pathogenicity tests conducted using mycelial plug inoculation identified the BJA1 strain from Beijing as the most virulent. Molecular identification through cloning and phylogenetic analysis of the ITS，TEF1，and GAPDH genes confirmed that BJA1 is a typical strain of Alternaria brassicicola. Using this strain，an efficient and stable in vitro inoculation system for evaluating resistance to cabbage black spot disease was established. The optimal spore concentration for inoculation was determined to be 1 × 10⁵ spores · mL^-1，and the ideal plant growth stage for inoculation was the four-leaf stage. This system was applied to evaluate the resistance of 100 cabbage germplasms to black spot disease，leading to the identification of 24 moderately resistant accessions and one resistant accession with a disease index of 25.93.

Key words: cabbage, black spot disease, Alternaria brassicicola, resistance evaluation, resistant source screening

王彩红, 张扬勇, 李森, 张朝文, 孔小平, 吕红豪, 王勇, 杨丽梅, 季家磊, 庄木. 甘蓝黑斑病菌鉴定、生物学特性分析及抗性种质资源筛选[J]. 园艺学报, 2026, 53(5): 1502-1520.

WANG Caihong, ZHANG Yangyong, LI Sen, ZHANG Chaowen, KONG Xiaoping, LÜ Honghao, WANG Yong, YANG Limei, JI Jialei, ZHUANG Mu. Identification of Black Spot Pathogen in Cabbage，Analysis of Biological Characteristics，and Screening of Resistant Germplasm Resources[J]. Acta Horticulturae Sinica, 2026, 53(5): 1502-1520.

图/表 16

参考文献 35

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引物 Primer	目标片段 Target region	序列 Primers sequence	参考文献 Reference
ITS	ITS1	5′-TCCTCCGCTTATTGATATGC-3′	White et al.,1990
ITS	ITS4	5′-CCGTAGGTGAACCTGCGG-3′	White et al.,1990
TEF1	TEF1	5′-CATCGAGAAGTTCGAGAAGG-3′	赵东航,2024
TEF1	TEF2	5′-TACTTGAAGGAACCCTTACC-3′	赵东航,2024
GAPDH	gpd1	5′-CAACGGCTTCGGTCGCATTG-3′	Berbee et al.,1999
GAPDH	gpd2	5′-GCCAAGCAGTTGGTTGTGC-3′	Berbee et al.,1999

引物 Primer	目标片段 Target region	序列 Primers sequence	参考文献 Reference
ITS	ITS1	5′-TCCTCCGCTTATTGATATGC-3′	White et al.,1990
ITS	ITS4	5′-CCGTAGGTGAACCTGCGG-3′	White et al.,1990
TEF1	TEF1	5′-CATCGAGAAGTTCGAGAAGG-3′	赵东航,2024
TEF1	TEF2	5′-TACTTGAAGGAACCCTTACC-3′	赵东航,2024
GAPDH	gpd1	5′-CAACGGCTTCGGTCGCATTG-3′	Berbee et al.,1999
GAPDH	gpd2	5′-GCCAAGCAGTTGGTTGTGC-3′	Berbee et al.,1999

基因 Gene	预变性 Initial denaturation	变性（循环次数35） Denaturation（Number of cycles 35）	退火 Annealing	延伸 Extension	补充延伸 Final extension
ITS	95 ℃，30 s	95 ℃，15 s	60 ℃，15 s	72 ℃，10 s	72 ℃，5 min
TEF1	95 ℃，30 s	95 ℃，15 s	60 ℃，15 s	72 ℃，10 s	72 ℃，5 min
GAPDH	94 ℃，5 min	94 ℃，15 s	58 ℃，15 s	72 ℃，20 s	72 ℃，5 min

基因 Gene	预变性 Initial denaturation	变性（循环次数35） Denaturation（Number of cycles 35）	退火 Annealing	延伸 Extension	补充延伸 Final extension
ITS	95 ℃，30 s	95 ℃，15 s	60 ℃，15 s	72 ℃，10 s	72 ℃，5 min
TEF1	95 ℃，30 s	95 ℃，15 s	60 ℃，15 s	72 ℃，10 s	72 ℃，5 min
GAPDH	94 ℃，5 min	94 ℃，15 s	58 ℃，15 s	72 ℃，20 s	72 ℃，5 min

抗性级别 Resistance level	病情指数 Disease index（DI）	抗性级别 Resistance level	病情指数 Disease index（DI）
高抗 Highly resistant（HR）	0.10 ~ 11.11	感病 Susceptible（S）	55.55 ~ 77.77
抗病 Resistant（R）	11.11 ~ 33.33	高感 Highly susceptible（HS）	77.77 ~ 100
中抗 Moderately resistant（MR）	33.33 ~ 55.55

甘蓝黑斑病菌鉴定、生物学特性分析及抗性种质资源筛选

Identification of Black Spot Pathogen in Cabbage，Analysis of Biological Characteristics，and Screening of Resistant Germplasm Resources

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菌株 Strain	菌落形态 Colony morphology	分生孢子特征 Conidium characterization
		形态 Morphological	孢子直径/μm Diameter of a spore	隔膜数 Number of diaphragms
		形态 Morphological	孢子直径/μm Diameter of a spore	横隔膜 Diaphragm	纵隔膜 Longitudinal diaphragm
SCA1	同心轮纹，中心呈黑褐色，外围呈均匀灰褐色，气生菌丝少，且分生孢子茂密Concentric rings with dark brown centers and uniformly gray-brown margins，exhibiting sparse aerial mycelium but abundant conidial production	倒棒状，无喙或不明显 Obclavate，beakless or with indistinct beak	（4.26 ~ 10.90）× （2.26 ~ 3.60）	1 ~ 5	0 ~ 1
SCA2	同心轮纹，菌落呈黑褐色，气生菌丝少，且分生孢子茂密Concentric rings with dark brown colonies，sparse aerial mycelium，and dense conidiation	倒棒状，无喙或不明显 Obclavate，beakless or with indistinct beak	（3.01 ~ 4.92）× （2.70 ~ 3.72）	1 ~ 4	0 ~ 5
SCB1	同心轮纹，中心呈黑褐色，外围呈均匀黄褐色，气生菌丝发达，且分生孢子茂密Concentric rings with dark brown centers，uniformly tan margins，abundant aerial mycelium，and dense conidiation	倒棒状或卵形，无喙或不明显Obclavate or ovoid，beakless or with indistinct beak	（3.33 ~ 12.11）× （2.47 ~ 2.91）	1 ~ 3	0 ~ 1
SCB2	同心轮纹，中心呈黑褐色，外围呈均匀灰褐色，气生菌丝少，且分生孢子茂密Concentric rings with dark brown centers，uniformly gray-brown margins，sparse aerial mycelium，and dense conidiation	倒棒状，无喙或不明显 Obclavate，beakless or with indistinct beak	（4.08 ~ 8.39）× （2.71 ~ 2.87）	1 ~ 3	0
SCC1	同心轮纹，中心呈黑褐色，外围呈均匀灰褐色，气生菌丝少，且分生孢子茂密Concentric rings with dark brown centers，uniformly gray-brown edges，sparse aerial hyphae，and dense conidia	倒棒状或卵形，无喙或不明显Obclavate or ovoid，beakless or with indistinct beak	（3.50 ~ 8.59）× （1.88 ~ 3.07）	1 ~ 3	0 ~ 1
SCC2	同心轮纹，中心呈黑褐色，外围呈均匀黄褐色，气生菌丝少，且分生孢子茂密Concentric rings with dark brown centers，uniformly tan margins，sparse aerial mycelium，and dense conidiation	倒棒状，无喙或不明显 Obclavate，beakless or with indistinct beak	（3.43 ~ 8.14）× （2.14 ~ 3.36）	1 ~ 4	0 ~ 4
NMA1	菌落呈黄白色，气生菌丝发达，且分生孢子茂密 Colonies appear yellowish-white with luxuriant aerial mycelium and dense conidiation	卵形或近球形，无喙或不明显Ovoid or subglobose，beakless or with indistinct beak	（2.75 ~ 4.10）× （2.27 ~ 3.50）	1 ~ 2	0 ~ 2
NMA2	菌落呈黄褐色和不均匀白色，气生菌丝发达，且分生孢子茂密Colonies exhibit tan and uneven white coloration with luxuriant aerial mycelium and dense conidiation	卵形或近球形，无喙或不明显Ovoid or subglobose，beakless or with indistinct beak	（2.60 ~ 4.51）× （1.84 ~ 2.80）	1 ~ 2	0 ~ 2
NMB1	菌落呈白色和不均匀黄褐色，气生菌丝发达，且分生孢子茂密Colonies appear white with uneven tan pigmentation，exhibiting luxuriant aerial mycelium and dense conidiation	卵形或近球形，无喙或不明显Ovoid or subglobose，beakless or with indistinct beak	（2.32 ~ 3.54）× （1.57 ~ 3.50）	1 ~ 2	0 ~ 2
NMB2	同心轮纹，菌落呈白色和黄褐色，气生菌丝发达，且分生孢子茂密 Concentric rings with white and tan colonies，luxuriant aerial mycelium，and dense conidiation	卵形或近球形，无喙或不明显Ovoid or subglobose，beakless or with indistinct beak	（2.68 ~ 3.32）× （1.79 ~ 2.55）	1 ~ 2	0 ~ 2
NMC1	菌落呈白色，气生菌丝发达，且分生孢子茂密White colonies with luxuriant aerial mycelium and dense conidiation	卵形或近球形，无喙或不明显Ovoid or subglobose，beakless or with indistinct beak	（2.37 ~ 4.75）× （1.61 ~ 2.73）	1 ~ 3	0 ~ 2
NMC2	菌落呈白色，气生菌丝发达，且分生孢子茂密White colonies with luxuriant aerial mycelium and dense conidiation	倒棒状，无喙或不明显 Obclavate，beakless or with indistinct beak	（3.14 ~ 7.67）× （2.08 ~ 2.54）	1 ~ 3	0 ~ 1
BJA1	同心轮纹，中心呈黑褐色，外围呈均匀灰褐色，气生菌丝少，且分生孢子茂密Concentric rings with dark brown centers，uniformly gray-brown margins，sparse aerial mycelium，and dense conidiation	倒棒状，无喙或不明显 Obclavate，beakless or with indistinct beak	（2.16 ~ 8.39）× （1.67 ~ 2.63）	1 ~ 3	0 ~ 1
BJA2	同心轮纹，中心呈黄褐色，外围不均匀黄褐色和白色，气生菌丝发达，且分生孢子茂密Concentric rings with tan centers，uneven tan and white margins，luxuriant aerial mycelium，and dense conidiation	倒棒状，无喙或不明显 Obclavate，beakless or with indistinct beak	（2.62 ~ 9.39）× （2.94 ~ 1.71）	1 ~ 3	0
BJB1	同心轮纹，中心呈黑褐色，外围呈均匀灰褐色，伴有不均匀白色菌落，气生菌丝发达，且分生孢子茂密Concentric rings with dark brown centers，uniformly gray-brown margins interspersed with irregular white colonies，exhibiting luxuriant aerial mycelium and dense conidiation	倒棒状，无喙或不明显 Obclavate，beakless or with indistinct beak	（2.40 ~ 7.00）× （1.49 ~ 3.29）	1 ~ 4	0~1
BJB1	同心轮纹，中心呈黄褐色，外围呈不均匀黑褐色，气生菌丝少，且分生孢子茂密Concentric rings with tan centers，unevenly dark brown margins，sparse aerial mycelium，and dense conidiation	倒棒状或卵形，无喙或短至不明显Obclavate or ovoid，beakless or with indistinct beak	（2.77 ~ 12.17）× （2.05 ~ 4.39）	1 ~ 3	0 ~ 1
BJC1	同心轮纹，中心呈黑褐色，外围呈均匀灰褐色，气生菌丝少，且分生孢子茂密Concentric rings with dark brown centers，uniformly gray-brown margins，sparse aerial mycelium，and dense conidiation	倒棒状或卵形，无喙或不明显Obclavate or ovoid，beakless or with indistinct beak	（3.13 ~ 14.13）× （1.67 ~ 4.64）	1 ~ 3	0 ~ 1
BJC2	同心轮纹，中心呈黑褐色，外围呈均匀灰褐色，气生菌丝少，且分生孢子茂密Concentric rings with dark brown centers，uniformly gray-brown margins，sparse aerial mycelium，and dense conidiation	倒棒状或卵形，无喙或不明显Obclavate or ovoid，beakless or with indistinct beak	（1.62 ~ 8.00）× （1.96 ~ 2.88）	1 ~ 3	0 ~ 1
HBA1	同心轮纹，中心呈黑褐色，外围呈均匀黄褐色，伴有不均匀白色菌落，气生菌丝发达，且分生孢子茂密 Concentric rings with dark brown centers，uniformly tan margins interspersed with irregular white colonies，exhibiting luxuriant aerial mycelium and dense conidiation	倒棒状或卵形，无喙或不明显Obclavate or ovoid，beakless or with indistinct beak	（3.07 ~ 5.88）× （1.67 ~ 2.28）	1 ~ 8	0 ~ 4
HBA2	同心轮纹，中心呈黑褐色，外围呈均匀黄褐色，伴有不均匀白色菌落，气生菌丝发达，且分生孢子茂密 Concentric rings with dark brown centers，uniformly tan margins featuring patchy white colonies，showing vigorous aerial mycelium and dense conidiation	倒棒状或卵形，无喙或不明显Obclavate or ovoid，beakless or with indistinct beak	（3.15 ~ 7.40）× （1.79 ~ 3.58）	1 ~ 8	0 ~ 4
HBB1	同心轮纹，中心呈灰白色，外围呈不均匀灰褐色，气生菌丝发达，且分生孢子茂密Concentric rings with gray-white centers，unevenly gray-brown margins，luxuriant aerial mycelium，and dense conidiation	倒棒状，无喙或不明显 Obclavate，beakless or with indistinct beak	（2.17 ~ 7.81）× （1.70 ~ 3.85）	1 ~ 8	0 ~ 4
HBB2	菌落呈黄褐色，伴有不均匀白色菌落，气生菌丝发达，且分生孢子茂密Tan colonies with irregular white sectors，exhibiting luxuriant aerial mycelium and dense conidiation	倒棒状或卵形，无喙或不明显Obclavate or ovoid，beakless or with indistinct beak	（3.31 ~ 6.11）× （1.09 ~ 2.16）	1 ~ 3	0 ~ 2
HBC1	菌落呈黄褐色，伴有不均匀白色菌落，气生菌丝发达，且分生孢子茂密Tan colonies with mottled white sectors，showing luxuriant aerial mycelium and dense conidiation	倒棒状或卵形，无喙或不明显Obclavate or ovoid，beakless or with indistinct beak	（2.47 ~ 7.17）× （1.71 ~ 3.28）	1 ~ 3	0 ~ 2
HBC2	菌落呈灰绿色，气生菌丝发达，且分生孢子茂密Gray- reen colonies with luxuriant aerial mycelium and dense conidiation	卵形或卵形，无喙或不明显 Ovoid or oval，beakless or with indistinct beak	（2.22 ~ 6.03）× （1.53 ~ 2.72）	1 ~ 3	0 ~ 5
CQA1	菌落呈灰色，气生菌丝发达，且分生孢子茂密 Gray colonies with luxuriant aerial mycelium and dense conidiation	倒棒状或卵形，无喙或不明显Obclavate or ovoid，beakless or with indistinct beak	（2.37 ~ 6.67）× （1.67 ~ 2.55）	1 ~ 3	0 ~ 2
CQA2	菌落多呈黄色，伴有不均匀白色菌落，气生菌丝发达，且分生孢子茂密Predominantly yellow colonies with irregular white sectors，exhibiting luxuriant aerial mycelium and dense conidiation	倒棒状，无喙或不明显 Obclavate，beakless or with indistinct beak	（3.77 ~ 4.73）× （1.89 ~ 2.78）	1 ~ 3	0 ~ 3
CQB1	菌落多呈黄色，伴有不均匀白色菌落，气生菌丝发达，且分生孢子茂密Colonies predominantly yellow with irregular white sectors，exhibiting luxuriant aerial mycelium and dense conidiation	倒棒状或卵形，无喙或不明显Obclavate or ovoid，beakless or with indistinct beak	（2.56 ~ 4.48）× （1.69 ~ 2.44）	1 ~ 3	0 ~ 3
CQB2	菌落呈均匀灰褐色，气生菌丝发达，且分生孢子茂密 Uniformly gray-brown colonies with luxuriant aerial mycelium and dense conidiation	卵形或近球形，无喙或不明显Ovoid or subglobose，beakless or with indistinct beak	（2.51 ~ 3.77）× （1.88 ~ 3.33）	1 ~ 3	0 ~ 3
CQC1	中心呈黄褐色，外围呈均匀灰白色，气生菌丝发达，且分生孢子茂密Tan centers with uniformly gray-white margins，exhibiting luxuriant aerial mycelium and dense conidiation	倒棒状，无喙或不明显 Obclavate，beakless or with indistinct beak	（2.41 ~ 7.43）× （1.84 ~ 3.02）	1 ~ 3	0 ~ 2
CQC2	中心呈黄褐色，外围呈均匀灰白色，气生菌丝发达，且分生孢子茂密Tan centers with uniformly gray-white margins，exhibiting luxuriant aerial mycelium and dense conidiation	倒棒状、卵形或近球形，无喙或不明显Obclavate，ovoid or subglobose，lacking a beak or with indistinct beak	（2.71 ~ 7.74）× （1.33 ~ 3.07）	1 ~ 3	0 ~ 2

菌株 Strains	主要产孢表型类型 Main spore-producing phenotypes
SCA1	次生分生孢子梗或孢子侧向分支，形成短孢子链 Secondary conidiophores or spores develop lateral branches，forming short spore chains
SCA2	高大乔木状的产孢表型 Tall arborescent sporulation phenotype
SCB1	矮树状分支的分生孢子短链 Short-branched dwarf-tree-like conidial chains
SCB2	分生孢子单生，分生孢子梗分支 Conidia single，conidiophores branched
SCC1	矮树状分支的分生孢子短链 Short-branched dwarf-tree-like conidial chains
SCC2	次生分生孢子梗或孢子侧向分支，形成短孢子链Secondary conidiophores or spores develop lateral branches，forming short spore chains
NMA1	分生孢子单生，分生孢子梗不分支 Conidia single，conidiophores unbranched
NMA2	分生孢子单生，分生孢子梗不分支 Conidia single，conidiophores unbranched
NMB1	分生孢子单生，分生孢子梗不分支 Conidia single，conidiophores unbranched
NMB2	分生孢子单生，分生孢子梗不分支 Conidia single，conidiophores unbranched
NMC1	分生孢子单生，分生孢子梗不分支 Conidia single，conidiophores unbranched
NMC2	次生分生孢子梗或孢子侧向分支，形成短孢子链 Secondary conidiophores or spores develop lateral branches，forming short spore chains
BJA1	矮树状分支的分生孢子短链 Short-branched dwarf-tree-like conidial chains
BJA2	矮树状分支的分生孢子短链 Short-branched dwarf-tree-like conidial chains
BJB1	矮树状分支的分生孢子短链 Short-branched dwarf-tree-like conidial chains
BJB1	分生孢子单生，分生孢子梗分支 Conidia single，conidiophores branched
BJC1	短或长，不分支的分生孢子链 Short or long，unbranched conidial chains
BJC2	次生分生孢子梗或孢子侧向分支，形成短孢子链Secondary conidiophores or spores develop lateral branches，forming short spore chains
HBA1	短或长，不分支的分生孢子链 Short or long，unbranched conidial chains
HBA2	短或长，不分支的分生孢子链 Short or long，unbranched conidial chains
HBB1	不分支或稀少短分支的长分生孢子链 Long conidial chains，unbranched or with sparse short branches
HBB2	短或长，不分支的分生孢子链 Short or long，unbranched conidial chains
HBC1	矮树状分支的分生孢子短链 Short-branched dwarf-tree-like conidial chains
HBC2	矮树状分支的分生孢子短链 Short-branched dwarf-tree-like conidial chains
CQA1	分生孢子单生，分生孢子梗分支 Conidia single，conidiophores branched
CQA2	矮树状分支的分生孢子短链 Short-branched dwarf-tree-like conidial chains
CQB1	次生分生孢子梗或孢子侧向分支，形成短孢子链Secondary conidiophores or spores develop lateral branches，forming short spore chains
CQB2	不分支或稀少短分支的长分生孢子链 Long conidial chains，unbranched or with sparse short branches
CQC1	分生孢子单生，分生孢子梗分支 Conidia single，conidiophores branched
CQC2	短或长，不分支的分生孢子链 Short or long，unbranched conidial chains

菌株 Strain	占比/% Ratio	菌株 Strain	占比/% Ratio	菌株 Strain	占比/% Ratio
SCA1	20.1	NMC2	18.4	CQA1	16.8
NMA1	17.7	BJA1	32.6	CQA2	5.1
NMA2	9.5	BJC1	23.4	CQB1	4.2
NMB2	9.8	BJC2	24.9	CQB2	6.7
NMC1	10.6	HBA1	19.4

编号 Number	材料 Material	人工离体接种鉴定 In vitro artificial inoculation evaluation		编号 Number	材料 Material	人工离体接种鉴定 In vitro artificial inoculation evaluation
编号 Number	材料 Material	病情指数 Disease index	抗病等级 Disease resistance rating	编号 Number	材料 Material	病情指数 Disease index	抗病等级 Disease resistance rating
AR1	秋德 Qiude	62.96	S	AR51	亚飞322 Yafei 322	66.67	S
AR2	明德 Mingde	85.19	HS	AR52	亚飞329 Yafei 329	74.07	S
AR3	湖月 Huyue	85.19	HS	AR53	嘉宝 Jiabao	85.19	HS
AR4	梦午台310 Mengwutai 310	81.48	HS	AR54	BI16	92.59	HS
AR5	YR梦午台307 YR Mengwutai 307	92.59	HS	AR55	99-109	66.67	S
AR6	奥289 Ao 289	51.85	MR	AR56	99-198	74.07	S
AR7	奥213 Ao 213	81.48	HS	AR57	绿缘355 Lüyuan 355	88.89	HS
AR8	奥214 Ao 214	85.19	HS	AR58	绿缘356 Lüyuan 356	96.30	HS
AR9	奥215 Ao 215	66.67	S	AR59	湖北1186-1 Hubei 1186-1	92.59	HS
AR10	寒泰 Hantai	70.37	S	AR60	觉绿甘蓝 Juelü Cabbage	62.96	S
AR11	春蓝296 Chunlan 296	44.44	MR	AR61	越色 Yuese	48.15	MR
AR12	春蓝297 Chunlan 297	48.15	MR	AR62	似珍奇 Sizhenqi	33.33	MR
AR13	春蓝300 Chunlan 300	88.89	HS	AR63	XL60	70.37	S
AR14	20-2-5	62.96	S	AR64	XLMN	37.04	MR
AR15	23202	66.67	S	AR65	YC205	55.56	S
AR16	21-3	55.56	S	AR66	YC207	81.48	HS
AR17	台41 Tai 41	85.19	MR	AR67	YC207	66.67	S
AR18	24-5	74.07	S	AR68	96-100	51.85	MR
AR19	富士早生 Early Fuji	59.26	S	AR69	M3	74.07	S
AR20	非洲甜牛心 African Sweet Oxheart	66.67	S	AR70	嘉丽绿 Jiali Green	66.67	S
AR21	夏结254 Xiajie 254	77.78	HS	AR71	嘉丽灰 Jiali Gray	77.78	HS
AR22	似柳生288 Siliusheng 288	81.48	HS	AR72	越冬1 Yuedong 1	70.37	S
AR23	似柳生289 Siliusheng 289	51.85	MR	AR73	AQN	59.26	S
AR24	14秋494 14 Qiu 494	55.56	S	AR74	湖北48 Hubei 48	70.37	S
AR25	媛春 Yuanchun	48.15	MR	AR75	寒玉26 Hanyu 26	70.37	S
AR26	Gunma	74.07	S	AR76	甘蓝543 Cabbage 543	62.96	S
AR27	西六277 Xiliu 277	37.04	MR	AR77	瑞甘22 Ruigan 22	62.96	S
AR28	070	74.07	S	AR78	春味 Chunwei	40.74	MR
AR29	Going	59.26	S	AR79	寒4 Han 4	33.33	MR
AR30	泽雅 Zeya	55.56	S	AR80	JY81	33.33	MR
AR31	约旦扁球 Jordan Oblate	88.89	HS	AR81	春旺 Chunwang	53.70	MR
AR32	西四270 Xisi 270	70.37	S	AR82	07Cb18	33.33	MR
AR33	双流 Shuangliu	88.89	HS	AR83	春晓 Chunxiao	77.78	HS
AR34	QD37	77.78	HS	AR84	YFW1	59.26	S
AR35	19czb-1	66.67	S	AR85	好味来485 Haoweilai 485	40.74	MR
AR36	21LB3-1	66.67	S	AR86	亚马逊 Amazonas	48.15	MR
AR37	21CQ-1	85.19	HS	AR87	湖北1186-2 Hubei 1186-2	77.78	HS
AR38	1039	74.07	S	AR88	湖北1186-3 Hubei 1186-3	66.67	S
AR39	CB201-516	85.19	HS	AR89	绿宝石 Emerald	81.48	HS
AR40	CB201-520	92.59	HS	AR90	A1438	51.85	MR
AR41	CB201-521	85.19	HS	AR91	好味来525 Haoweilai 525	40.74	MR
AR42	希望720 Xiwang 720	59.26	S	AR92	米兰 Milan	62.96	S
AR43	希望721 Xiwang 721	70.37	S	AR93	A620	81.48	HS
AR44	吉宝 Jibao	48.15	MR	AR94	625甘蓝 625 Cabbage	77.78	HS
AR45	YFW2	77.78	HS	AR95	A901	51.85	MR
AR46	17HN82	66.67	S	AR96	A902	70.37	S
AR47	美一番 Meiyifan	62.96	S	AR97	C320	66.67	S
AR48	强绿625 Qianglü 625	66.67	S	AR98	牛心1 Oxheart 1	44.44	MR
AR49	天宝绿福 Tianbaolüfu	25.93	R	AR99	牛心10 Oxheart 10	55.56	S
AR50	T1000	37.04	MR	AR100	牛心33 Oxheart 33	66.67	S

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甘蓝黑斑病菌鉴定、生物学特性分析及抗性种质资源筛选

Identification of Black Spot Pathogen in Cabbage，Analysis of Biological Characteristics，and Screening of Resistant Germplasm Resources

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