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园艺学报 ›› 2026, Vol. 53 ›› Issue (1): 82-98.doi: 10.16420/j.issn.0513-353x.2025-0094

• 遗传育种·种质资源·分子生物学 • 上一篇    下一篇

菜薹TCP家族基因鉴定及其对腋芽性状的影响

朱彩烁1,2,*, 李建忠2,3,*, 张倩男2,4, 徐丽杰2,5, 张淑江2, 章时蕃2, 李菲2, 张慧2, 徐进1, 宋子涵2,**(), 李国亮2,**()   

  1. 1 山西农业大学园艺学院, 山西太谷 030801
    2 中国农业科学院蔬菜花卉研究所蔬菜生物育种全国重点实验室, 北京 100081
    3 安徽农业大学园艺学院, 合肥 230036
    4 宁夏农林科学院固原分院, 宁夏固原 756000
    5 沈阳农业大学林学院, 沈阳 110866
  • 收稿日期:2025-04-17 修回日期:2025-09-19 出版日期:2026-01-25 发布日期:2026-01-26
  • 通讯作者:
    **E-mail:
  • 作者简介:

    *共同第一作者

  • 基金资助:
    重庆市自然科学基金(CSTB2025NSCQ-GPX0681); 中国农业科学院创新工程项目(CAAS-ASTIP-IVFCAAS); 农业农村部园艺作物生物学与种质创制重点实验室项目; 现代农业产业技术体系建设专项资助(CARS-23-A-14)

Identification of the TCP Gene Family in Brassica campestris and Analysis of Their Effects on Axillary Bud Traits

ZHU Caishuo1,2, LI Jianzhong2,3, ZHANG Qiannan2,4, XU Lijie2,5, ZHANG Shujiang2, ZHANG Shifan2, LI Fei2, ZHANG Hui2, XU Jin1, SONG Zihan2,**(), LI Guoliang2,**()   

  1. 1 College of HorticultureShanxi Agricultural University,Taigu, Shanxi 030801, China
    2 State Key Laboratory of Vegetable BiobreedingInstitute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3 College of HorticultureAnhui Agricultural University, Hefei 230036, China
    4 Guyuan BranchNingxia Academy of Agricultural and Forestry Sciences,Guyuan, Ningxia 756000, China
    5 College of ForestryShenyang Agricultural University, Shenyang 110866, China
  • Received:2025-04-17 Revised:2025-09-19 Published:2026-01-25 Online:2026-01-26

摘要:

解析TCP基因家族调控菜薹腋芽形成的机制,对于改良菜薹株型具有重要意义。基于拟南芥TCP家族成员与菜薹基因组的同源关系,筛选并鉴定出菜薹TCP家族成员,进行理化性质、系统发育关系、基因结构、染色体定位、共线性分析,同时对不同品种的菜薹材料中TCP家族基因的表达量进行分析,进一步筛选出与菜薹腋芽发育相关的关键基因进行亚细胞定位分析。结果表明,在菜薹中共鉴定出39个TCP家族成员,均包含保守的TCP结构域。TCP家族基因在染色体上分布不均匀。通过选择腋芽数量不同的4种菜薹材料进行实时荧光定量分析,发现TCP家族中的BraA05g005380.3C、BraA07g034590.3C、BraA03g048240.3C、BraA02g018710.3C、BraA03g038400.3C和BraA01g034360.3C具有明显的表达量差异,推测这些基因是正向调控菜薹腋芽发育的关键候选基因。对上述6个基因进行亚细胞定位分析发现,BraA07g034590.3C基因定位在细胞核膜上,其余基因均定位在细胞核中。

关键词: 菜薹, TCP, 基因家族, 腋芽, 调控基因

Abstract:

Elucidating the mechanism by with the TCP gene family regulates of axillary buds in Brassica campestris is important for enhancing the plant architecture of B. campestris. Based on the homology between Arabidopsis TCP family members and the Brassica rapa genome,the TCP family members of Brassica campestris were screened and identified for physicochemical properties, phylogenetic relationships,gene structure,chromosomal localization,and covariance analysis,and the expression of TCP family genes in different Brassica campestris materials was also analyzed,and key genes related to the development of axillary buds of Brassica campestris were further identified for subcellular localization analysis. The results showed that a total of 39 TCP family members were identified in Brassica campestris,all of which contained conserved TCP structural domains. the TCP family genes were distributed unevenly on the chromosome. By selecting four kinds of Brassica campestris materials with different numbers of axillary buds for real-time fluorescence quantitative analysis,it was found that the TCP family of BraA05g005380.3C,BraA07g034590.3C,BraA03g048240.3C,BraA02g018710.3C,BraA03g038400.3C,and BraA01g034360.3C had significant expression differences,and it was hypothesized that these genes were key candidate genes for positively regulating axillary bud growth in Brassica campestris. Subcellular localization analysis of the above six genes revealed that the BraA07g034590.3C gene was localized to the nuclear membrane of the cell,while the rest of the genes were localized in the nucleus.

Key words: Brassica campestris, TCP, gene family, axillary bud, regulation gene