桃JMJ组蛋白去甲基化酶家族基因鉴定及表达分析

doi:10.16420/j.issn.0513-353x.2024-0105

园艺学报 ›› 2025, Vol. 52 ›› Issue (3): 575-590.doi: 10.16420/j.issn.0513-353x.2024-0105

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

桃JMJ组蛋白去甲基化酶家族基因鉴定及表达分析

汪芸芸¹^,²^,^*, 周晖²^,^*, 邱可立¹, 潘海发², 盛玉², 石佩², 谢庆梅², 陈红莉², 张金云²^,^**(), 李大辉¹^,^**()

¹ 安徽农业大学生命科学学院，合肥 230036
² 安徽省农业科学院园艺研究所，农业农村部园艺作物种质创制与利用重点实验室（省部共建），合肥 230001

收稿日期:2024-10-25 修回日期:2025-01-14 出版日期:2025-03-25 发布日期:2025-03-25
通讯作者:
**E-mail：zjy600@aaas.org.cn；
ldh@ahau.edu.cn
作者简介:
^*共同第一作者
基金资助:
淮北市重大科技专项(HK2021017); 安徽省果树产业技术体系项目(AHNYCYTX-10); 现代农业产业技术体系建设专项资助(CARS-30-Z-05)

Identification and Expression Analysis of Peach JMJ Histone Demethylase Gene Family

WANG Yunyun¹^,², ZHOU Hui², QIU Keli¹, PAN Haifa², SHENG Yu², SHI Pei², XIE Qingmei², CHEN Hongli², ZHANG Jinyun²^,^**(), LI Dahui¹^,^**()

¹ College of Life Sciences，Anhui Agricultural University，Hefei 230036，China
² Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops，Ministry of Agriculture and Rural Affairs，Institute of Horticulture，Anhui Academy of Agricultural Sciences，Hefei 230001，China

Received:2024-10-25 Revised:2025-01-14 Published:2025-03-25 Online:2025-03-25

摘要/Abstract

摘要：

为深入了解组蛋白甲基化在桃树生长发育和应对非生物胁迫中的功能，对含有JmjC结构域的组蛋白去甲基化酶（JMJ）家族基因进行了全基因组鉴定。在桃树基因组中鉴定到了21个JMJ，根据它们的系统发育关系分为5个亚家族。这些家族成员之间存在较大的差异，基因外显子数在7 ~ 33，蛋白中包含12个保守结构域，并且基因启动子上游区域含有不同数量的胁迫响应、激素响应和发育调控元件。选取PpJMJ5作为诱饵，通过酵母双杂交筛库发现了一个潜在互作蛋白PpCIPK1。通过克隆全长进行酵母双杂交试验，证明PpJMJ5与PpCIPK1存在互作关系，可能在一定程度上干扰钙调磷酸酶B类似蛋白（CBL）与其互作蛋白（CIPK）之间的相互作用。PpJMJ5可能在桃树的非生物胁迫响应中发挥重要作用。

关键词: 桃, 组蛋白去甲基化酶, 非生物胁迫, JMJ

Abstract:

In order to understand the function of histone methylation in growth and response to abiotic stress in peach trees，genome-wide identification of histone demethylase（JMJ）family genes containing the JmjC domain was performed. Twenty-one JMJs were identified in the peach genome，which were divided into five subfamilies according to their phylogenetic relationships. There were significant differences among the members of these families，with exons ranging from 7 to 33，proteins containing 12 conserved domains，and the upstream regions of gene promoters containing different numbers of stress response，hormone response，and developmental regulatory elements. Using PpJMJ5 as bait，a potential interacting protein PpCIPK1 was found through yeast two-hybrid screening library. It was proved that the interaction between PpJMJ5 and PpCIPK1 existed，which may interfere with the interaction between calhomodin and its interacting protein CBL-CIPK to some extent. PpJMJ5 may play an important role in the abiotic stress response of peach trees.

Key words: peach, histone demethylation, abiotic stress, JMJ

汪芸芸, 周晖, 邱可立, 潘海发, 盛玉, 石佩, 谢庆梅, 陈红莉, 张金云, 李大辉. 桃JMJ组蛋白去甲基化酶家族基因鉴定及表达分析[J]. 园艺学报, 2025, 52(3): 575-590.

WANG Yunyun, ZHOU Hui, QIU Keli, PAN Haifa, SHENG Yu, SHI Pei, XIE Qingmei, CHEN Hongli, ZHANG Jinyun, LI Dahui. Identification and Expression Analysis of Peach JMJ Histone Demethylase Gene Family[J]. Acta Horticulturae Sinica, 2025, 52(3): 575-590.

图/表 13

参考文献 36

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用途 Purpose	名称 Name	上游引物（5′-3′） Forward primer sequence	下游引物（3′-5′） Reverse primer sequence
qRT-PCR	qPpJMJ5	CTGAATTGCGAAATAGGCTGG	GCCCCTGGAAGGACAAGAA
酵母双杂 Yeast two-hybrid	pGBKT7-PpJMJ5	ATGGCCATGGAGGCCGAATTCATGGCTTTTAATTCAGTTCCACCT	CCGCTGCAGGTCGACGGATCCTCACCTTTGCCTGGATGTTGA
酵母双杂 Yeast two-hybrid	pGADT7-PpJMJ5	GCCATGGAGGCCAGTGAATTCATGGCTTTTAATTCAGTTCCACCT	ATTCATCTGCAGCTCGAGCTCTCACCTTTGCCTGGATGTTGA
	pGBKT7-PpCBL5	ATGGCCATGGAGGCCGAATTCATGGGTTGTTTTAGTTCTAAAGCAA	CCGCTGCAGGTCGACGGATCCACGTAGCAATCTCATCAACCT
	pGADT7-PpCBL5	GCCATGGAGGCCAGTGAATTCATGGGTTGTTTTAGTTCTAAAGCAA	ATTCATCTGCAGCTCGAGCTCTCACGTAGCAATCTCATCAACCT
	pGBKT7-PpCIPK1	ATGGCCATGGAGGCCGAATTCATGGACCAAGGGGTCCCA	CCGCTGCAGGTCGACGGATCCTCAAACGACGACGTTATGCC
	pGADT7-PpCIPK1	GCCATGGAGGCCAGTGAATTCATGGACCAAGGGGTCCCA	ATTCATCTGCAGCTCGAGCTCTCAAACGACGACGTTATGCC
	pGADT7-PpCIPK2	GCCATGGAGGCCAGTGAATTCATGGGACTTGCCAGCAACA	ATTCATCTGCAGCTCGAGCTCTCAAGACGATGAATAGCCACG
	pGADT7-PpCIPK3	GCCATGGAGGCCAGTGAATTCATGGTGATCGTAAGAAAAGGTGC	ATTCATCTGCAGCTCGAGCTCTCACACTTGGCTAGTCAACAACC
	pGADT7-PpCIPK4	GCCATGGAGGCCAGTGAATTCATGGAAGAAAGGACTGTCTTGTTT	ATTCATCTGCAGCTCGAGCTCTCATGAGTCTTGTTGATCACCCT
	pGADT7-PpCIPK5	GCCATGGAGGCCAGTGAATTCATGGTGGTGAGGAAAGTGGGTA	ATTCATCTGCAGCTCGAGCTCTCAACGCTTTTTACTTTTACTCTTGC
	pGADT7-PpCIPK6	GCCATGGAGGCCAGTGAATTCATGGAGAAGATGAGTGCGGC	ATTCATCTGCAGCTCGAGCTCTTATTTCCTTGATCCTTCAGTAGTTTC
	pGADT7-PpCIPK7	GCCATGGAGGCCAGTGAATTCATGGAAAATAAGGGTAAGGTGTTGA	ATTCATCTGCAGCTCGAGCTCTTACTGTTTCTCCTCACCTTGCC
	pGADT7-PpCIPK10	GCCATGGAGGCCAGTGAATTCATGGCAAACCTCACCACCA	ATTCATCTGCAGCTCGAGCTCCTATTCAGAATCTGAAGGTAGATATGAAG
	pGADT7-PpCIPK11	GCCATGGAGGCCAGTGAATTCATGTCGTCGAGGTCGGCG	ATTCATCTGCAGCTCGAGCTCTCAAGATGGCAGAGCACCG
	pGADT7-PpCIPK12	GCCATGGAGGCCAGTGAATTCATGCCAGAGATCGAACAGCAG	ATTCATCTGCAGCTCGAGCTCTCAATCAGCTGGGCAGTGC
	pGADT7-PpCIPK13	GCCATGGAGGCCAGTGAATTCATGGAGAATAAATCGAATGTGCTG	ATTCATCTGCAGCTCGAGCTCCTATTGTGGCTGATGCTGTTCTAGT
	pGADT7-PpCIPK14	GCCATGGAGGCCAGTGAATTCATGAATCAACCAAAAATCA AGCG	ATTCATCTGCAGCTCGAGCTCTTACTTCACTTTTTGCATATCCTCCT
	pGADT7-PpCIPK15	GCCATGGAGGCCAGTGAATTCATGGAAAACAAAGGGAGTG TGTT	ATTCATCTGCAGCTCGAGCTCTTATGAATCCTGGGGCGAGA
	pGADT7-PpCIPK16	GCCATGGAGGCCAGTGAATTCATGCCGGAGATCGAGGTAGT	ATTCATCTGCAGCTCGAGCTCTTAATTACCGGCGACGTCC
	pGADT7-PpCIPK17	GCCATGGAGGCCAGTGAATTCATGAAGAAGGTGTCGAGAA AGGTAG	ATTCATCTGCAGCTCGAGCTCTCAGCAAGTCATTGTTCGAAGC
	pGADT7-PpCIPK18	GCCATGGAGGCCAGTGAATTCATGGCGGATGAAAAAGGCA	ATTCATCTGCAGCTCGAGCTCTCAAGCAGCCGTCGAACTG
	pGBKT7-PpCIPK1-1	ATGGCCATGGAGGCCGAATTCATGGACCAAGGGGTCCCAC	CCGCTGCAGGTCGACGGATCCTCATACGGCGTCGCATTTCGA
	pGADT7-PpCIPK1-1	GCCATGGAGGCCAGTGAATTCATGGACCAAGGGGTCCCAC	ATTCATCTGCAGCTCGAGCTCTCATACGGCGTCGCATTTCGA
	pGBKT7-PpCIPK1-2	ATGGCCATGGAGGCCGAATTCGAGGAGGAGAAGTCGAAAT	CCGCTGCAGGTCGACGGATCCTCAAACGACGACGTTATGCCA
	pGADT7-PpCIPK1-2	GCCATGGAGGCCAGTGAATTCGAGGAGGAGAAGTCGAAATGCG	ATTCATCTGCAGCTCGAGCTCTCAAACGACGACGTTATGCCA
LCI	PpJMJ5-Cluc	TACGCGTCCCGGGGCGGTACCATGGCTTTTAATTCAGTTCCACCT	ACGAAAGCTCTGCAGGTCGACTCACCTTTGCCTGGATGTTGA
LCI	PpCIPK1-Nluc	ACGGGGGACGAGCTCGGTACCATGGACCAAGGGGTCCCAC	CGCGTACGAGATCTGGTCGACAACGACGACGTTATGCCACG

用途 Purpose	名称 Name	上游引物（5′-3′） Forward primer sequence	下游引物（3′-5′） Reverse primer sequence
qRT-PCR	qPpJMJ5	CTGAATTGCGAAATAGGCTGG	GCCCCTGGAAGGACAAGAA
酵母双杂 Yeast two-hybrid	pGBKT7-PpJMJ5	ATGGCCATGGAGGCCGAATTCATGGCTTTTAATTCAGTTCCACCT	CCGCTGCAGGTCGACGGATCCTCACCTTTGCCTGGATGTTGA
酵母双杂 Yeast two-hybrid	pGADT7-PpJMJ5	GCCATGGAGGCCAGTGAATTCATGGCTTTTAATTCAGTTCCACCT	ATTCATCTGCAGCTCGAGCTCTCACCTTTGCCTGGATGTTGA
	pGBKT7-PpCBL5	ATGGCCATGGAGGCCGAATTCATGGGTTGTTTTAGTTCTAAAGCAA	CCGCTGCAGGTCGACGGATCCACGTAGCAATCTCATCAACCT
	pGADT7-PpCBL5	GCCATGGAGGCCAGTGAATTCATGGGTTGTTTTAGTTCTAAAGCAA	ATTCATCTGCAGCTCGAGCTCTCACGTAGCAATCTCATCAACCT
	pGBKT7-PpCIPK1	ATGGCCATGGAGGCCGAATTCATGGACCAAGGGGTCCCA	CCGCTGCAGGTCGACGGATCCTCAAACGACGACGTTATGCC
	pGADT7-PpCIPK1	GCCATGGAGGCCAGTGAATTCATGGACCAAGGGGTCCCA	ATTCATCTGCAGCTCGAGCTCTCAAACGACGACGTTATGCC
	pGADT7-PpCIPK2	GCCATGGAGGCCAGTGAATTCATGGGACTTGCCAGCAACA	ATTCATCTGCAGCTCGAGCTCTCAAGACGATGAATAGCCACG
	pGADT7-PpCIPK3	GCCATGGAGGCCAGTGAATTCATGGTGATCGTAAGAAAAGGTGC	ATTCATCTGCAGCTCGAGCTCTCACACTTGGCTAGTCAACAACC
	pGADT7-PpCIPK4	GCCATGGAGGCCAGTGAATTCATGGAAGAAAGGACTGTCTTGTTT	ATTCATCTGCAGCTCGAGCTCTCATGAGTCTTGTTGATCACCCT
	pGADT7-PpCIPK5	GCCATGGAGGCCAGTGAATTCATGGTGGTGAGGAAAGTGGGTA	ATTCATCTGCAGCTCGAGCTCTCAACGCTTTTTACTTTTACTCTTGC
	pGADT7-PpCIPK6	GCCATGGAGGCCAGTGAATTCATGGAGAAGATGAGTGCGGC	ATTCATCTGCAGCTCGAGCTCTTATTTCCTTGATCCTTCAGTAGTTTC
	pGADT7-PpCIPK7	GCCATGGAGGCCAGTGAATTCATGGAAAATAAGGGTAAGGTGTTGA	ATTCATCTGCAGCTCGAGCTCTTACTGTTTCTCCTCACCTTGCC
	pGADT7-PpCIPK10	GCCATGGAGGCCAGTGAATTCATGGCAAACCTCACCACCA	ATTCATCTGCAGCTCGAGCTCCTATTCAGAATCTGAAGGTAGATATGAAG
	pGADT7-PpCIPK11	GCCATGGAGGCCAGTGAATTCATGTCGTCGAGGTCGGCG	ATTCATCTGCAGCTCGAGCTCTCAAGATGGCAGAGCACCG
	pGADT7-PpCIPK12	GCCATGGAGGCCAGTGAATTCATGCCAGAGATCGAACAGCAG	ATTCATCTGCAGCTCGAGCTCTCAATCAGCTGGGCAGTGC
	pGADT7-PpCIPK13	GCCATGGAGGCCAGTGAATTCATGGAGAATAAATCGAATGTGCTG	ATTCATCTGCAGCTCGAGCTCCTATTGTGGCTGATGCTGTTCTAGT
	pGADT7-PpCIPK14	GCCATGGAGGCCAGTGAATTCATGAATCAACCAAAAATCA AGCG	ATTCATCTGCAGCTCGAGCTCTTACTTCACTTTTTGCATATCCTCCT
	pGADT7-PpCIPK15	GCCATGGAGGCCAGTGAATTCATGGAAAACAAAGGGAGTG TGTT	ATTCATCTGCAGCTCGAGCTCTTATGAATCCTGGGGCGAGA
	pGADT7-PpCIPK16	GCCATGGAGGCCAGTGAATTCATGCCGGAGATCGAGGTAGT	ATTCATCTGCAGCTCGAGCTCTTAATTACCGGCGACGTCC
	pGADT7-PpCIPK17	GCCATGGAGGCCAGTGAATTCATGAAGAAGGTGTCGAGAA AGGTAG	ATTCATCTGCAGCTCGAGCTCTCAGCAAGTCATTGTTCGAAGC
	pGADT7-PpCIPK18	GCCATGGAGGCCAGTGAATTCATGGCGGATGAAAAAGGCA	ATTCATCTGCAGCTCGAGCTCTCAAGCAGCCGTCGAACTG
	pGBKT7-PpCIPK1-1	ATGGCCATGGAGGCCGAATTCATGGACCAAGGGGTCCCAC	CCGCTGCAGGTCGACGGATCCTCATACGGCGTCGCATTTCGA
	pGADT7-PpCIPK1-1	GCCATGGAGGCCAGTGAATTCATGGACCAAGGGGTCCCAC	ATTCATCTGCAGCTCGAGCTCTCATACGGCGTCGCATTTCGA
	pGBKT7-PpCIPK1-2	ATGGCCATGGAGGCCGAATTCGAGGAGGAGAAGTCGAAAT	CCGCTGCAGGTCGACGGATCCTCAAACGACGACGTTATGCCA
	pGADT7-PpCIPK1-2	GCCATGGAGGCCAGTGAATTCGAGGAGGAGAAGTCGAAATGCG	ATTCATCTGCAGCTCGAGCTCTCAAACGACGACGTTATGCCA
LCI	PpJMJ5-Cluc	TACGCGTCCCGGGGCGGTACCATGGCTTTTAATTCAGTTCCACCT	ACGAAAGCTCTGCAGGTCGACTCACCTTTGCCTGGATGTTGA
LCI	PpCIPK1-Nluc	ACGGGGGACGAGCTCGGTACCATGGACCAAGGGGTCCCAC	CGCGTACGAGATCTGGTCGACAACGACGACGTTATGCCACG

亚家族 Subfamily	基因 Gene	ID	染色体位置 Chromosomal location	蛋白质长度/aa Protein length	等电点pI	亚细胞定位 Subcellular localization
KDM4/HDM3	PpJMJ1	Prupe.2G211400	2	1 553	6.08	细胞核Cell nucleus
	PpJMJ2	Prupe.5G159200	5	1 486	8.73	细胞核Cell nucleus
	PpJMJ3	Prupe.5G028800	5	893	8.27	细胞核Cell nucleus
KDM5/JARID	PpJMJ4	Prupe.6G349900	6	1 227	6.26	细胞核Cell nucleus
	PpJMJ5	Prupe.8G222100	8	709	7.93	细胞核Cell nucleus
	PpJMJ6	Prupe.4G102200	4	1 067	5.64	细胞核Cell nucleus
	PpJMJ7	Prupe.8G028600	8	461	6.15	细胞核Cell nucleus
	PpJMJ8	Prupe.5G099000	5	1 851	6.66	细胞核Cell nucleus
JMJD6	PpJMJ9	Prupe.1G521300	1	1 001	5.33	细胞质Cytoplasm
	PpJMJ10	Prupe.7G089800	7	521	5.91	线粒体Mitochondria
	PpJMJ11	Prupe.6G322900	6	942	5.78	细胞核Cell nucleus
KDM3/JHDM2	PpJMJ12	Prupe.2G123400	2	853	8.70	细胞核Cell nucleus
	PpJMJ13	Prupe.5G015100	5	979	6.72	细胞核Cell nucleus
	PpJMJ14	Prupe.4G016300	4	867	5.60	细胞核Cell nucleus
	PpJMJ15	Prupe.2G309900	2	1 770	8.12	细胞核Cell nucleus
	PpJMJ16	Prupe.2G189600	2	1 051	6.86	细胞核Cell nucleus
	PpJMJ17	Prupe.4G034800	4	1 031	8.56	细胞核Cell nucleus
	PpJMJ18	Prupe.5G169900	5	480	5.09	细胞质Cytoplasm
JMJC domain-only	PpJMJ19	Prupe.6G015700	6	407	5.37	细胞核Cell nucleus
	PpJMJ20	Prupe.8G234700	8	556	5.11	细胞核Cell nucleus
	PpJMJ21	Prupe.4G147600	4	342	5.18	细胞核Cell nucleus

亚家族 Subfamily	基因 Gene	ID	染色体位置 Chromosomal location	蛋白质长度/aa Protein length	等电点pI	亚细胞定位 Subcellular localization
KDM4/HDM3	PpJMJ1	Prupe.2G211400	2	1 553	6.08	细胞核Cell nucleus
	PpJMJ2	Prupe.5G159200	5	1 486	8.73	细胞核Cell nucleus
	PpJMJ3	Prupe.5G028800	5	893	8.27	细胞核Cell nucleus
KDM5/JARID	PpJMJ4	Prupe.6G349900	6	1 227	6.26	细胞核Cell nucleus
	PpJMJ5	Prupe.8G222100	8	709	7.93	细胞核Cell nucleus
	PpJMJ6	Prupe.4G102200	4	1 067	5.64	细胞核Cell nucleus
	PpJMJ7	Prupe.8G028600	8	461	6.15	细胞核Cell nucleus
	PpJMJ8	Prupe.5G099000	5	1 851	6.66	细胞核Cell nucleus
JMJD6	PpJMJ9	Prupe.1G521300	1	1 001	5.33	细胞质Cytoplasm
	PpJMJ10	Prupe.7G089800	7	521	5.91	线粒体Mitochondria
	PpJMJ11	Prupe.6G322900	6	942	5.78	细胞核Cell nucleus
KDM3/JHDM2	PpJMJ12	Prupe.2G123400	2	853	8.70	细胞核Cell nucleus
	PpJMJ13	Prupe.5G015100	5	979	6.72	细胞核Cell nucleus
	PpJMJ14	Prupe.4G016300	4	867	5.60	细胞核Cell nucleus
	PpJMJ15	Prupe.2G309900	2	1 770	8.12	细胞核Cell nucleus
	PpJMJ16	Prupe.2G189600	2	1 051	6.86	细胞核Cell nucleus
	PpJMJ17	Prupe.4G034800	4	1 031	8.56	细胞核Cell nucleus
	PpJMJ18	Prupe.5G169900	5	480	5.09	细胞质Cytoplasm
JMJC domain-only	PpJMJ19	Prupe.6G015700	6	407	5.37	细胞核Cell nucleus
	PpJMJ20	Prupe.8G234700	8	556	5.11	细胞核Cell nucleus
	PpJMJ21	Prupe.4G147600	4	342	5.18	细胞核Cell nucleus

桃JMJ组蛋白去甲基化酶家族基因鉴定及表达分析

Identification and Expression Analysis of Peach JMJ Histone Demethylase Gene Family

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桃JMJ组蛋白去甲基化酶家族基因鉴定及表达分析

Identification and Expression Analysis of Peach JMJ Histone Demethylase Gene Family

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图/表 13

参考文献 36

相关文章 15

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