沃柑实生后代三倍体发掘及遗传鉴定

doi:10.16420/j.issn.0513-353x.2023-0522

园艺学报 ›› 2024, Vol. 51 ›› Issue (10): 2231-2242.doi: 10.16420/j.issn.0513-353x.2023-0522

• 遗传育种·种质资源·分子生物学 • 下一篇

沃柑实生后代三倍体发掘及遗传鉴定

冯意斯¹^,^*, 田啸宇¹^,^*, 杨崇山¹, 陈香玲³, 邓秀新¹^,², 解凯东¹, 郭文武¹^,², 谢宗周¹, 柴利军¹, 叶俊丽¹^,^**()

¹ 华中农业大学园艺林学学院，果蔬园艺作物种质创新与利用全国重点实验室，武汉 430070
² 湖北省洪山实验室，武汉 430070
³ 广西农业科学院园艺研究所，南宁 530007

收稿日期:2024-07-26 修回日期:2024-09-04 出版日期:2024-10-25 发布日期:2024-10-21
通讯作者:
**E-mail：yejunli@mail.hzau.edu.cn
作者简介:
^*共同第一作者
基金资助:
南宁市科技重大专项(20222061); 现代农业产业技术体系建设专项资助(CARS26)

Exploitation and Genetic Identification of Triploid Plants from Seedling Populations of Orah Mandarin（Citrus reticulata）

FENG Yisi¹, TIAN Xiaoyu¹, YANG Chongshan¹, CHEN Xiangling³, DENG Xiuxin¹^,², XIE Kaidong¹, GUO Wenwu¹^,², XIE Zongzhou¹, CHAI Lijun¹, YE Junli¹^,^**()

¹ National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops，College of Horticulture and Forestry，Huazhong Agricultural University，Wuhan 430070，China
² Hubei Hongshan Laboratory，Wuhan 430070，China
³ Horticultural Research Institute，Guangxi Academy of Agricultural Sciences，Nanning 530007，China

Received:2024-07-26 Revised:2024-09-04 Published:2024-10-25 Online:2024-10-21

摘要/Abstract

摘要：

从柑橘二倍体实生后代中发掘天然三倍体是培育无核新品种的有效手段。对沃柑饱满小种子进行催芽和实生播种，通过形态学初筛，结合流式细胞仪和染色体计数法进行倍性鉴定，共获得98个三倍体株系。利用2对在母本及候选父本间具有多态性的SSR引物对子代三倍体的来源进行分析，在三倍体子代中均仅能检测到与沃柑相同的条带。进一步利用36对S-RNase引物进行遗传分析，结果显示，三倍体子代中均出现了沃柑S基因型S₁₁/S_M，其中2个株系还出现了候选父本砂糖橘的S基因型S₂₂，推测这两个株系为沃柑与砂糖橘杂交产生的三倍体子代，且由2n雌配子受精形成，其余株系为沃柑自交产生的同源三倍体。与同时期沃柑二倍体幼苗相比，三倍体幼苗生长缓慢，节间距变短，矮化明显，主根短粗，须根少，叶色浓绿且叶片较厚。同时，异源三倍体植株叶片中多个胁迫响应相关基因（CitCOMT、CitGRAS、CitERF4和CitERF9）的表达均较二倍体和同源三倍体显著上调。

关键词: 沃柑, 实生后代, 三倍体, 遗传分析

Abstract:

Discovering natural triploids from diploid seedling offspring is an effective mehod to cultivate new seedless citrus cultivar. In this study，the small plump seeds of Orah were germinated and sowed，and the ploidy of seedling offsprings was identified by morphological screening，flow cytometry and chromosome counting. A total of 98 triploid lines of Orah seedlings were obtained. Two pairs of SSR primers with polymorphism between the female parent and its candidate male parent were used to analyze the hybrid origin of triploid progenies. It was found that only the amplification product of Orah mandarin could be detected in the triploid progenies. Further genetic analysis was performed using 36 pairs of S-RNase primers. Results showed that the S genotype S₁₁/S_Mof Orah mandarin was detected in all triploid progenies，and the S genotype S₂₂ of the candidate male parent Shatangju occurred in two lines，presumed that these two lines were triploid progenies produced by the hybridization of Orah and Shatangju，and were formed by fertilization of 2n female gametes. The remaining lines might be autotriploids produced by selfing of Orah. Compared with the diploid seedlings，the triploid seedlings of the same age grew slowly，the internodes became shorter，the dwarfing was obvious，the main roots were short and thick，the fibrous roots were less，the leaves were dark green and thicker. Under the same cultivation and management conditions，the expression of multiple stress response related genes（CitCOMT，CitGRAS，CitERF4 and CitERF9）in the leaves of allotriploid plants was significantly up-regulated compared with diploid and autotriploid plants.

Key words: Orah, seedling progeny, triploid, genetic analysis

冯意斯, 田啸宇, 杨崇山, 陈香玲, 邓秀新, 解凯东, 郭文武, 谢宗周, 柴利军, 叶俊丽. 沃柑实生后代三倍体发掘及遗传鉴定[J]. 园艺学报, 2024, 51(10): 2231-2242.

FENG Yisi, TIAN Xiaoyu, YANG Chongshan, CHEN Xiangling, DENG Xiuxin, XIE Kaidong, GUO Wenwu, XIE Zongzhou, CHAI Lijun, YE Junli. Exploitation and Genetic Identification of Triploid Plants from Seedling Populations of Orah Mandarin（Citrus reticulata）[J]. Acta Horticulturae Sinica, 2024, 51(10): 2231-2242.

图/表 8

表1 用于基因表达分析的qPCR引物序列

Table 1 Primer sequences for qPCR analysis

基因 Gene	NCBI编号 Accession ID	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
COMT	XM_052435554	F：TTGAAACCAGCAAGGGATGAG	R：GCGCCGGAAGCGAGTT
PUB19	XM_006483767	F：GGGTCGCCGGGTACTGA	R：TTGACGGAGAAATGGATTCACA
ERF4	XM_006464742	F：TCCCTCCTCCACCTGAAGTG	R：CGCCGTACACGTCAGCATTA
ERF9	XM_006467686	F：TGGTGCCGGTGGAGGAT	R：CCACAGGCTGGAAGGGAAA
TCF1	XM_052439239	F：GGCTCTATGGACAGTGTGGG	R：GTCACCATCGCTGGAAATGC
ERF6	XM_006477063	F：AGGCGATAACGGCGACA	R：AAAGCCAAGGCGGTGAA
GRAS	XM_006475224	F：ATGTCGCTCCACCCTCT	R：TACTGCCGCATTCCTCC
Actin	GU911361	F：CCAAGCAGCATGAAGATCAA	R：ATCTGCTGGAAGGTGCTGAG

图1 沃柑的果实（a）、种子（b）和实生苗（c）

Fig. 1 Fruit（a），seed（b）and seedling（c）of Orah mandarin

图2 沃柑二倍体（2x）与疑似三倍体（3x）的叶片（a）、根系（b）和叶片油胞（c、d）

Fig. 2 Leaf（a），root（b）and leaf oil gland（c，d）of diploid（2x）and suspected triploid（3x）of Orah mandarin

图3 沃柑疑似三倍体实生后代的流式细胞仪倍性鉴定（a、b）和根尖染色体计数（c） a：二倍体；b：三倍体；c：三倍体根尖染色体数2n = 3x = 27。

Fig. 3 Ploidy identification by flow cytometry（a，b）and root tip chromosome count（c）of suspected triploid seedlings of Orah mandarin a：Diploid；b：Triploid；c：Triploid root tip chromosome number 2n = 3x = 27.

图4 母本沃柑（♀）、候选父本（♂）和三倍体植株（3x）毛细管电泳核酸分析 a：引物Csin0342；b：引物Csin0087。M：沃柑；G：茂谷柑；H：红江橙；B：白心橙。

Fig. 4 Capillary electrophoresis nucleic acid analysis of female parent Orah mandarin（♀），candidate male parent（♂）and triploid plant（3x） a：Primer Csin0342；b：Primer Csin0087. M：Orah mandarin；G：Murcott；H：Hongjiangcheng；B：Baixincheng.

图5 母本沃柑（♀，M）、候选父本（♂）及三倍体后代（3x，1 ~ 93）S-RNase基因特异性扩增

Fig. 5 The specific amplification of S-RNase gene of Orah mandarin（♀，M），triploid plants（3x，1-93）and their candidate male parent（♂）

图6 沃柑三倍体（3x）与二倍体（2x）幼苗植株的叶片、根系、枝刺和节间距比较分析

Fig. 6 Comparison of leaf，roots，branch thorns and internode of triploid（3x）and diploid（2x）plants of Orah mandarin t-test，* P < 0.05，** P < 0.01.

图7 沃柑二倍体、同源三倍体、异源三倍体叶片中胁迫相关基因的表达量差异

Fig. 7 Differential expression analysis of stress related genes in leaves of Orah mandarin diploid，autotriploid and allotriploid by qPCR One-way ANOVA. n = 3，P < 0.05.

参考文献 36

[1]	Ahmad M H, Zheng X, Hu Y, Liu H M, Sun Y, Wen H, Chai L J. 2023. Determination of self-(In)compatibility and inter-(In)compatibility relationships in citrus using manual pollination,microscopy,and S-genotype analyses. Jove-Journal of Visualized Experiments,Doi:10.3791/65056-v.
[2]	Allario T, Brumos J, Colmenero-Flores J M, Iglesias D J, Pina J A, Navarro L, Talon M, Ollitrault P, Morillon R. 2013. Tetraploid Rangpur lime rootstock increases drought tolerance via enhanced constitutive root abscisic acid production. Plant Cell and Environment,36:856-868.
[3]	Chen Chuanwu, Niu Ying, Fu Huimin, Liu Binghao, Fan Qijun, Jiang Hailan. 2022. Study on the cause of seedlessness of‘Seedless Orah’. Fruit Trees in Southern China, 51 (2):1-7. (in Chinese)
	陈传武, 牛英, 付慧敏, 刘冰浩, 范七君, 蒋海兰. 2022. “无核沃柑”无核成因研究. 中国南方果树, 51 (2):1-7.
[4]	Cheng Yunjiang, Guo Wenwu, Yi Hualin, Pang Xiaoming, Deng Xiuxin. 2003. An efficient protocol for genomic DNA extraction from Citrus species. Plant Molecular Biology Reporter, 21 (2):177-178.
[5]	Cohen H, Fait A, Tel-Zur N. 2013. Morphological,cytological and metabolic consequences of autopolyploidization in Hylocereus(Cactaceae) species. BMC Plant Biology,13:173.
[6]	Cuenca J, Aleza P, Juarez J, Garcia-Lor A, Froelicher Y, Navarro L, Ollitrault P. 2015. Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci:application in citrus. Scientific Reports,Doi:10.1038/srep09897.
[7]	Deng Xiuxin, Guo Wenwu, Sun Xuhua. 1996. Research progress on breeding of seedless citrus in China-literature review. Journal of Horticulture, 23 (3):29-34. (in Chinese)
	邓秀新, 郭文武, 孙绪华. 1996. 我国无核柑桔类型选育研究进展——文献综述. 园艺学报, 23 (3):29-34.
[8]	Fasano C, Diretto G, Aversano R, D'Agostino N, Di M A, Frusciante L, Giuliano G, Carputo D. 2016. Transcriptome and metabolome of synthetic Solanum autotetraploids reveal key genomic stress events following polyploidization. New Phytologist,210:1382-1394.
[9]	Huang Haisheng, Tang Dongjian. 2015. The planting performance,early bearing,high yield and high quality cultivation techniques of Orah in Nanning. Acta Guangxi Agricultural,(30):56-59. (in Chinese)
	黄海生, 唐东坚. 2015. 沃柑在南宁种植表现及早结丰产优质栽培技术. 广西农学报,(30):56-59.
[10]	Huang Qichun, Jiang Tangxin, Qin Yang, Li Deguang, Liu Jimin, Zhang Lan, Shi Pingli, Liao Huihong, Liu Yaoxin, Chen Dongkui. 2020. Experimental performance of 091 seedless Orah in Nanning,Guangxi. South China Fruit Tree,49:17-20. (in Chinese)
	黄其椿, 江唐鑫, 覃阳, 李德光, 刘吉敏, 张兰, 施平丽, 廖惠红, 刘要鑫, 陈东奎. 2020. 091无核沃柑在广西南宁的试种表现. 中国南方果树,49:17-20.
[11]	Ji H, Wang Y, Cloix C, Li K, Jenkins G I, Wang S, Shang Z, Shi Y, Yang S, Xia L. 2015. The Arabidopsis RCC 1 family protein TCF1 regulates freezing tolerance and cold acclimation through modulating lignin biosynthesis. PLoS Genetics, 11 (9):e1005471.
[12]	Jiang Dong, Cao Li. 2011. Introduction performance of late-maturing high-sugar hybrid citrus cultivar‘Orah’in Chongqing. South China Fruit Tree,(40):33-34. (in Chinese)
	江东, 曹立. 2011. 晚熟高糖杂柑品种“沃柑”在重庆的引种表现. 中国南方果树,(40):33-34.
[13]	Kuwahara M, Kusano N. 2013. Production of triploid trifoliate orange hybrids for citrus rootstock. IEEE Conference on Emerging Technologies & Factory Automation. IEEE,1-8.
[14]	Liang M, Cao Z H, Zhu A D, Liu Y L, Tao M Q, Yang H Y, Wang S H, Liu J J, Li Y P, Chen C W, Xie Z H, Deng C L, Ye J L, Guo W W, Xu Q, Xia Rui, Larkin R M, Deng X X, Bosch M, Franklin-Tong V E, Chai L J. 2020. Evolution of self-compatibility by a mutant Sm-RNase in citrus. Nature Plants,6:131-142.
[15]	Liang Wujun, Xie Kaidong, Guo Dayong, Xie Zongzhou, Xu Qiang, Yi Hualin, Guo Wenwu. 2014. Exploration and SSR identification of polyploids from seedlings of 10 citrus varieties. Acta Horticulturae Sinica, 41 (3):409-416. (in Chinese)
	梁武军, 解凯东, 郭大勇, 谢宗周, 徐强, 伊华林, 郭文武. 2014. 柑橘10个品种实生后代多倍体的发掘及SSR鉴定. 园艺学报, 41 (3):409-416.
[16]	Liu B, Xu C, Zhao N, Qi B, Kimatu J N, Pang J, Han F. 2009a. Rapid genomic changes in polyploid wheat and related species:implications for genome evolution and genetic improvement. Journal of Genetics and Genomics,36:519-528.
[17]	Liu Q, Zhu A, Chai L, Zhou W, Yu K, Ding J, Xu J, Deng X. 2009b. Transcriptome analysis of a spontaneous mutant in sweet orange[Citrus sinensis (L.) Osbeck] during fruit development. Journal of Experimental Botany, 60 (3):801-813.
[18]	Liu Y C, Wu Y R, Huang X H, Sun J, Xie Q. 2011. AtPUB19,a U-box E 3 ubiquitin ligase,negatively regulates abscisic acid and drought responses in Arabidopsis thaliana. Molecular Plant, 4 (6):938-946.
[19]	Maruyama Y, Yamoto N, Suzuki Y, Chiba Y, Yamazaki K, Sato T, Yamaguchi J. 2013. The Arabidopsis transcriptional repressor ERF 9 participates in resistance against necrotrophic fungi. Plant Sci,213:79-87.
[20]	Mu H Z, Liu Z J, Lin L, Li H Y, Jiang J, Liu G F. 2012. Transcriptomic analysis of phenotypic changes in Birch(Betula platyphylla) autotetraploids. International Journal of Molecular Sciences,13:13012-13029.
[21]	Peng Kang. 2022. S-genotypes identification and self-compatibility evaluation of citrus germplasms[M. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	彭康. 2022. 柑橘S基因型鉴定及亲和资源发掘[硕士论文]. 武汉: 华中农业大学.
[22]	Seo Y J, Park J B, Cho Y J, Jung C, Seo H S, Park S K, Nahm B H, Song J T. 2010. Overexpression of the ethylene-responsive factor gene BrERF4 from Brassica rapa increases tolerance to salt and drought in Arabidopsis plants. Molecular Cell,30:271-277.
[23]	Sewelam N, Kazan K, Thomas-Hall S R, Kidd B N, Manners J M, Schenk P M. 2013. Ethylene response factor 6 is a regulator of reactive oxygen species signaling in Arabidopsis. Public Library of Science, 8 (8):e70289.
[24]	Song Jiankun. 2006. Creation and genetic analysis of citrus triploid germplasm resources[Ph. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	宋健坤. 2006. 柑橘三倍体种质资源的创造及遗传分析[博士论文]. 武汉: 华中农业大学.
[25]	Sudo M, Yasuda K, Yahata M, Sato M, Tominaga A, Mukai H, Ma G, Kato M, Kunitake H. 2021. Morphological characteristics,fruit qualities and evaluation of reproductive functions in autotetraploid Satsuma Mandarin(Citrus unshiu Marcow.). Agronomy-Basel, 11 (12):2441.
[26]	Vincent D, Lapierre C, Pollet B, Cornic G, Negroni L, Zivy M. 2005. Water deficits affect caffeate O-methyltransferase,lignification,and related enzymes in maize leaves. A proteomic investigation. Plant Physiology,137:949-960.
[27]	Wang X, Wang H, Shi C, Zhang X, Duan K, Luo J. 2015. Morphological,cytological and fertility consequences of a spontaneous tetraploid of the diploid pear(Pyrus pyrifolia Nakai)cultivar‘Cuiguan’. Scientia Horticulturae,189:59-65.
[28]	Wang Yuxuan. 2022. Study on the effect of spraying gibberellin and cytokinin on fruit preservation of seedless mandarin[M. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	王煜轩. 2022. 喷施赤霉素和细胞分裂素对无核沃柑保果效果的研究[硕士论文]. 武汉: 华中农业大学.
[29]	Wang Z, Liu L, Cheng C, Ren Z, Xu S, Li X. 2020. GAI Functions in the plant response to dehydration stress in Arabidopsis thaliana. International Journal of Molecular Sciences, 21 (3):819.
[30]	Wu J H, Ferguson A R, Murray B G, Jia Y L, Datson P M, Zhang J L. 2012. Induced polyploidy dramatically increases the size and alters the shape of fruit in Actinidia chinensis. Annals of Botany,109:169-179.
[31]	Xia Q M, Wang W, Xie K D, Wu X M, Deng X X, Grosser J W, Guo W W. 2021. Unreduced megagametophyte formation via second division restitution contributes to tetraploid production in interploidy crosses with‘Orah’mandarin(Citrus reticulata). Frontiers of Agricultural Science & Engineering, 8 (2):302-313.
[32]	Xie Qingfeng. 2017. High-yielding cultivation and management techniques for high-quality kumquat. Southern Agriculture,(11):20-21. (in Chinese)
	谢庆丰. 2017. 优质沃柑丰产栽培管理技术. 南方农业,(11):20-21
[33]	Xie Shanpeng, Xie Kaidong, Xia Qiangming, Zhou Rui, Zhang Chenglei, Zheng Hao, Wu Xiaomeng, Guo Wenwu. 2022. Efficient exploration and SSR identification of 53 doubled diploid seed-lings from six local Citrus cultivars and germplasm resources. Journal of Fruit Science, 39 (1):1-9. (in Chinese)
	谢善鹏, 解凯东, 夏强明, 周锐, 张成磊, 郑浩, 伍小萌, 郭文武. 2022. 柑橘 6个地方品种资源四倍体高效发掘及分子鉴定. 果树学报, 39 (1):1-9.
[34]	Younis A, Hwang Y J, Lim K B. 2014. Exploitation of induced 2n-gametes for plant breeding. Plant Cell Reports,33:215-223.
[35]	Zhang J, Liu Y, Xia E H, Yao Q Y, Liu X D, Gao L Z. 2015. Autotetraploid rice methylome analysis reveals methylation variation of transposable elements and their effects on gene expression. Proceedings of the National Academy of Sciences of the United States of America,112:E7022-E7029.
[36]	Zhou Rui, Xie Kaidong, Wang Wei, Peng Jun, Xie Shanpeng, Hu Yibo, Wu Xiaomeng, Guo Wenwu. 2020. According to the morphological characteristics of polyploidy,citrus tetraploids were quickly and efficiently excavated. Acta Horticulturae Sinica, 47 (12):2451-2458. (in Chinese)
	周锐, 解凯东, 王伟, 彭珺, 谢善鹏, 胡益波, 伍小萌, 郭文武. 2020. 依据多倍体形态特征快速高效发掘柑橘四倍体. 园艺学报, 47 (12):2451-2458.

沃柑实生后代三倍体发掘及遗传鉴定

Exploitation and Genetic Identification of Triploid Plants from Seedling Populations of Orah Mandarin（Citrus reticulata）

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 36

相关文章 15

编辑推荐

Metrics

本文评价

访问总数:　当日访问总数:　当前在线人数:

[1]	曹惠祥, 罗鑫, 刘婉荣, 管书萍, 王婷婷, 伍小萌, 郭文武, 解凯东. 柑橘2个三倍体果实囊衣发育与化渣性评价[J]. 园艺学报, 2024, 51(6): 1179-1188.
[2]	刘春洋, 彭朝凤, 程世平, 姚鹏强, 耿喜宁, 谢丽华. 高温诱导‘凤丹’牡丹2n雌配子创制三倍体[J]. 园艺学报, 2023, 50(7): 1455-1466.
[3]	李淑培, 张映, 舒金帅, 陈钰辉, 杨锦坤, 陈露露, 刘富中. 茄子叶色黄化突变体chl234的特性及其叶绿体超微结构[J]. 园艺学报, 2023, 50(5): 1000-1008.
[4]	袁储聪, 苏江硕, 蒋甲福, 邓波, 张飞, 管志勇, 房伟民, 陈发棣. 切花菊F₁代光反应周期的遗传表现[J]. 园艺学报, 2023, 50(10): 2104-2116.
[5]	师校欣, 杜国强, 杨丽丽, 乔月莲, 黄成立, 王素月, 赵跃欣, 魏晓慧, 王莉, 齐向丽. 晚熟无核葡萄新品种‘红峰无核’[J]. 园艺学报, 2022, 49(S2): 39-40.
[6]	程世平, 姚鹏强, 耿喜宁, 刘春洋, 谢丽华. 高温诱导牡丹产生未减数花粉[J]. 园艺学报, 2022, 49(3): 581-589.
[7]	党江波, 党江波, 韩国辉, 李彩, 向素琼, 郭启高, 梁国鲁. 柑橘倍性操作技术与育种现状、难点及展望[J]. 园艺学报, 2021, 48(4): 791-810.
[8]	周熠玮, 许国宇, 王琴, 严福龙, 玉云祎, 余让才, 范燕萍. ‘白姜花’×‘金姜花’杂交F₁代花色遗传分析及其相关SSR分子标记开发[J]. 园艺学报, 2021, 48(10): 1921-1933.
[9]	刘明秀, 王泓丁, 张宇娜, 张帮燕, 刘松, 李韵佳, 党江波, 何桥, 梁国鲁, 郭启高. 三倍体枇杷与其四倍体和二倍体亲本光系统活性差异[J]. 园艺学报, 2021, 48(1): 37-48.
[10]	王五宏，汪精磊，李必元，魏庆镇，胡天华，胡海娇，包崇来*. 结球甘蓝抽薹性遗传规律和QTL定位分析[J]. 园艺学报, 2020, 47(5): 974-982.
[11]	张莹1，曹玉芬1,*，田路明1，董星光1，霍宏亮1，李树玲2，黄礼森1，赵德英1，齐丹1，徐家玉1，闫帅1，王立东1. 三倍体梨新品种‘华香酥’[J]. 园艺学报, 2020, 47(5): 1009-1010.
[12]	孙伟雄，石敏，薛宝贵，夏燕，郭启高，王淑明，吴頔，景丹龙*，梁国鲁. 三倍体枇杷花期调控基因 EjSPL5 的克隆、亚细胞定位及表达分析[J]. 园艺学报, 2020, 47(2): 220-232.
[13]	刘富中, 张映, 杨锦坤, 陈钰辉, 舒金帅, 李淑培, 陈露露. 茄子叶色黄化突变体的特征及遗传分析[J]. 园艺学报, 2020, 47(12): 2340-2348.
[14]	李国亮，张淑江，钱伟，李菲，章时蕃，张慧，方智远，孙日飞*. 大白菜抗TuMV显性位点F2的QTL-seq分析[J]. 园艺学报, 2019, 46(2): 307-316.
[15]	薛一花，何琼，陈霖，李茹，张鲁刚*. 大白菜紫心性状的遗传分析及度量参数比较[J]. 园艺学报, 2019, 46(12): 2423-2428.

沃柑实生后代三倍体发掘及遗传鉴定

Exploitation and Genetic Identification of Triploid Plants from Seedling Populations of Orah Mandarin（Citrus reticulata）

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 36

相关文章 15

编辑推荐

Metrics

本文评价

访问总数: 当日访问总数: 当前在线人数:

访问总数:　当日访问总数:　当前在线人数: