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园艺学报 ›› 2021, Vol. 48 ›› Issue (10): 1921-1933.doi: 10.16420/j.issn.0513-353x.2021-0624

• 研究论文 • 上一篇    下一篇

‘白姜花’ב金姜花’杂交F1代花色遗传分析及其相关SSR分子标记开发

周熠玮1,2, 许国宇1,2, 王琴1,2, 严福龙1,2, 玉云祎1,2, 余让才2,3, 范燕萍1,2,**()   

  1. 1华南农业大学林学与风景园林学院,广州510642
    2华南农业大学花卉研究中心,广州 510642
    3华南农业大学生命科学学院,广州 510642
  • 收稿日期:2021-08-06 修回日期:2021-09-10 出版日期:2021-10-25 发布日期:2021-11-01
  • 通讯作者: 范燕萍 E-mail:fanyanping@scau.edu.cn
  • 基金资助:
    广东省重点领域研发计划项目(2020B020220007);国家自然科学基金项目(31770738);广州市民生科技重点攻关计划项目(201903010054)

Genetic Analysis and Development of Associated SSR Markers of the Flower Color in F1 Population of Hedychium coronarium‘COR01’× H. ‘Jin’

ZHOU Yiwei1,2, XU Guoyu1,2, WANG Qin1,2, YAN Fulong1,2, YU Yunyi1,2, YU Rangcai2,3, FAN Yanping1,2,**()   

  1. 1College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China
    2Flower Research Center Guangzhou 510642,China
    3College of Life Science,South China Agricultural University,Guangzhou 510642,China
  • Received:2021-08-06 Revised:2021-09-10 Online:2021-10-25 Published:2021-11-01
  • Contact: FAN Yanping E-mail:fanyanping@scau.edu.cn

摘要:

以‘白姜花’为母本,‘金姜花’为父本杂交获得的114株F1杂交后代为材料,对唇瓣主色、唇瓣心部主色、花丝主色和花药主色等4个重要花色性状进行观测和分析,同时利用集团分离分析法开发花色相关分子标记。结果显示,114株F1杂交后代花色分离广泛,唇瓣主色、唇瓣心部主色、花丝主色和花药主色的香农—维纳指数为0.57 ~ 1.58。唇瓣主色和唇瓣心部主色的L*a*b*值呈连续分布。聚类分析可将114个F1杂交后代分为橙色系和白色系两大类。BSA-SSR分析从191对引物中确定了HcgSSR676标记与花色性状紧密相关,该标记与白色系/橙色系,唇瓣主色黄度值(b*1)、唇瓣心部主色亮度值(L*2)、红度值(a*2)、黄度值(b*2)极显著相关,回归决定系数R2范围为7.92 ~ 23.19。

关键词: 姜花, 花色, 遗传分析, SSR, BSA

Abstract:

In this study,114 F1 hybrids derived from the cross between Hedychium coronarium‘COR01’and H.‘Jin’were used as materials. Four important flower color-related traits including the dominant color of labellum,labellum heart,filament,and anther were observed and analysed. Meanwhile,bulked segregant analysis(BSA)was used to develop color-related molecular markers. The results showed that the flower color of 114 F1 hybrids was widely separated,and the Shannon-Wiener index of the main color of labellum petal,labellum heart,filament,and anther ranged from 0.57 to 1.58. Colorimeter analysis indicated that the L*,a* and b*values of the dominant color of labellum and labellum heart were continuously distributed. Cluster analysis revealed that 114 F1 hybrids could be divided into white group and orange group. BSA-SSR analysis confirmed that the HcgSSR676 marker screened out from 191 primers pairs was closely related to flower color traits. The marker was significantly associated with the white/orange group,yellowness value of labellum dominant color(b*1),brightness value(L*2),redness value(a*2),and yellowness value(b*2)of labellum heart dominant color,whose regression determination coefficient(R2)ranged from 7.92 to 23.19.

Key words: Hedychium, flower color, genetic analysis, SSR, BSA

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