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园艺学报 ›› 2019, Vol. 46 ›› Issue (9): 1655-1669.doi: 10.16420/j.issn.0513-353x.2019-0237

• 综述 • 上一篇    下一篇

植物类黄酮生物合成相关UDP–糖基转移酶研究进展

解林峰,任传宏,张 波,徐昌杰,李 鲜*   

  1. 浙江大学园艺系,浙江省园艺植物整合生物学研究与应用重点实验室,杭州 310058
  • 出版日期:2019-09-25 发布日期:2019-09-25
  • 基金资助:
    国家自然科学基金项目(31872067);国家重点研发计划项目(2016YFD0400106)

Plant UDP-glycosyltransferases in Flavonoids Biosynthesis

XIE Linfeng,REN Chuanhong,ZHANG Bo,XU Changjie,and LI Xian*   

  1. Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,Department of Horticulture,Zhejiang University,Hangzhou 310058,China
  • Online:2019-09-25 Published:2019-09-25

摘要: 类黄酮是人类膳食和观赏园艺植物中重要的多酚类物质,因其广泛的生物活性及重要的生物学功能而倍受关注。植物尿苷二磷酸糖基转移酶(Uridine diphosphate glycosyltransferase,UGT)催化类黄酮生物合成的最后一步从而形成多种多样的糖苷衍生物。近年来,大量UGT基因家族被分析,类黄酮合成相关糖基转移酶也陆续被鉴别报道。本文综述了植物类黄酮生物合成相关UGT的生物化学及分子生物学研究进展,重点对参与不同类黄酮糖基化的UGT体外重组蛋白活性及转基因功能分析等试验证据进行归纳,并介绍了相关的微生物工程,以期为植物类黄酮代谢和其调控原理及生物技术研究提供参考。

关键词: 类黄酮, 生物合成, UDP–糖基转移酶, 生物学功能

Abstract: Flavonoids are important polyphenols for human diet and ornamental horticulture. Flavonoids attract attention for their wide range of biological activities and important biological functions. Uridine diphosphate glycosyltransferases(UGT) catalyze the final step of flavonoid biosynthesis to form various glycoside derivatives. Recently,with the development of plant genomics,many flavonoid biosynthesis related UGTs were analyzed and identified. Here,research progress on plant UGTs involved in flavonoids glycosylation modification was summarized from biochemical and molecular biological points of view. Important evidence illustrating enzymatic activities of flavonoid biosynthesis related-UGTs,functional characterization,as well as bioengineering of flavonoids with key UGTs were listed,which may provide theoretical foundation for regulation on flavonoid biosynthesis in the future.

Key words: flavonoid, biosynthesis, UDP-glycosyltransferase, biological function

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