园艺学报 ›› 2019, Vol. 46 ›› Issue (6): 1192-1200.doi: 10.16420/j.issn.0513-353x.2018-0648

• 研究报告 • 上一篇    下一篇



  1. 1南京林业大学风景园林学院,南京 210037;2江苏省农业科学院休闲农业研究所,南京 210014
  • 出版日期:2019-06-25 发布日期:2019-06-25
  • 基金资助:

Analysis of Different Expressed Genes of Rhododendrons Under Salinity Stress by cDNA-SCoT

ZHENG Fuchao1,GENG Xingmin1,*,YUE Yuanzheng1,SU Jiale2,and JIA Xinping2   

  1. 1College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037,China;2Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China
  • Online:2019-06-25 Published:2019-06-25

摘要: 土壤盐渍是限制杜鹃属(Rhododendron L.)植物分布与园林应用的主要因素之一。为探究杜鹃在盐胁迫中的抗性分子机理,以2个杜鹃品种‘江南春早’(R.‘Jiangnan Chunzao’)和‘胭脂密’(R. obtusu‘Yanzhi Mi’)为材料,进行盐胁迫处理,然后采用cDNA-SCoT的方法进行差异表达基因的筛选。结果表明,40个引物能够扩增出90多条带,其长度在100 ~ 1 800 bp之间;筛选出32条差异明显的条带进行回收,连接转化,经测序得到了15条高质量的EST序列。其生物信息分析表明,杜鹃盐胁迫下的差异基因主要编码过氧化氢酶、LRR受体样丝氨酸/苏氨酸蛋白激酶At1g63430异构体X1、Shc相关蛋白、光系统Ⅱ蛋白D1以及PTS果糖转运蛋白亚基ⅡABC等功能性蛋白,涉及细胞结构、防御反应、代谢、能量代谢、信号转导、转运、转录等。

关键词: 杜鹃, 盐胁迫, cDNA-SCoT, 差异表达基因

Abstract: Soil salinization is one of the main factors that limit the distribution and landscape application of Rhododendron. To explore the molecular mechanism of Rhododendron resistance to salt stress,R.‘Jiangnan Chunzao’and R. obtusu‘Yanzhi Mi’seedlings were used as the materials to analyse different expressed genes under salt stress by the method of cDNA-SCoT. The results showed that 40 primers could amplify more than 90 bands,most of which were between 100-1800 bp in length. Thirty two bands with obvious differences were selected for recovery. Fifteen high-quality EST sequences were obtained by sequencing after the transformation. Bioinformatics analysis showed that the differential genes in Rhododendron seedlings exposed to salt stress mainly coded catalase,LRR receptor-like serine / threonine protein kinase At1g63430 Structure X1,Shc-related protein,photosystem Ⅱ protein D1,PTS fructose transporter subunit ⅡABC etc,and involved in cell structure,defense response,metabolism,energy metabolism,signal transduction,transport and transcription etc.

Key words: Rhododendron, salt stress, cDNA-SCoT, different expressed genes