园艺学报 ›› 2016, Vol. 43 ›› Issue (10): 2029-2038.doi: 10.16420/j.issn.0513-353x.2016-0252

• 研究报告 • 上一篇    下一篇



  1. (西北农林科技大学园艺学院,农业部西北地区园艺作物生物学与种质资源创新重点实验室,陕西杨凌 712100)
  • 出版日期:2016-10-25 发布日期:2016-10-25

Cloning and Expression Analysis of BrLOM2,a Gene Involved in Lobed Leaf Development in Pakchoi

ZHANG Shu-juan,YAN Fang-fang,YU Zhao-jun,ZHANG Ming-ke,and HUI Mai-xia*   

  1. (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China of Ministry of Agriculture,College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China)
  • Online:2016-10-25 Published:2016-10-25


为了揭示白菜(Brassica rapa ssp. chinensisBrLOM2基因在叶发育中的调控机制,以白菜圆叶、裂叶近等基因系为材料,同源克隆得到BrLOM2基因,分析其时空表达特性及对激素的响应。结果显示,BrLOM2的ORF全长为549 bp,编码182个氨基酸,含有GRAS家族保守结构域,定位于细胞核,两种材料中的氨基酸序列在保守结构域存在两处突变。进化分析表明,BrLOM2属于十字花科LOM2分支,与大白菜(XP_009138823.1)亲缘关系最近。RT-PCR结果显示,BrLOM2在圆叶白菜中的表达量高于同期裂叶白菜,两种材料茎尖、第1片叶中表达量最高;裂叶中的表达量与叶缘裂刻数呈现同步增加的趋势,6-BA和GA3分别抑制和促进叶片中的表达,说明BrLOM2低表达可能是裂叶表型产生的主要原因。

关键词: 白菜, LOM2, 裂叶, 基因克隆, 表达特性


To elucidate the mechanism underlying development of leaf marginal lobes in pakchoi,an LOM2 homologous gene,BrLOM2,was isolated respectively from near-isogenic lines with and without lobed leaves in pakchoi using the homology-based cloning methods. The BrLOM2 gene contains an open reading frame of 549 bp in length encoding a protein of 182 amino acids. Sequence analysis showed that the BrLOM2 protein contained conservative structure domains of the GRAS family. The BrLOM2 sequence from genomic DNA showed no intron and phylogenetic analysis suggested that BrLOM2shares high homology with known LOMs from other plant species. Moreover,the putative BrLOM2 protein was closely clustered with several Brassica LOMs. qRT-PCR analysis revealed that BrLOM2gene has higher expression in leaves and shoot apexes,and lower expression in flowers. Further moreBrLOM2 gene has higher expression level in pakchoi lines with smooth leaves than in the lines with lobed leaves. The expression of BrLOM2 in pakchoi lines with lobed leaves became higher,while the number of lobes in them had the synchronized trend. In leaves,the expression of BrLOM2 was affected by application of exogenous GA3 and 6-BA hormone treatments. InterestinglyGA3 treatment increased the BrLOM2gene expression,whereas 6-BA treatment decreased it. In summary,the above-mentioned results indicated that the reduced transcriptional expression of BrLOM2 gene was the major factor contributing to leaf marginal lobes.

Key words: Brassica rapa ssp. chinensis, LOM2, lobed leaf, gene clone, expression pattern