园艺学报 ›› 2015, Vol. 42 ›› Issue (5): 879-889.doi: 10.16420/j.issn.0513-353x.2014-0966

• 蔬菜 • 上一篇    下一篇



  1. 1江西省农业科学院蔬菜花卉研究所,南昌 330200;2江西省农业科学院油料作物重点实验室,南昌 330200;3江西农业大学农学院,南昌 330045
  • 出版日期:2015-05-25 发布日期:2015-05-25
  • 基金资助:


Detection of QTLs for Main Agronomic Traits Using F2 and F2:3 Interspecific Populations in Pepper

ZHOU Kun-Hua, LEI Gang, FANG Rong, CHEN Xue-Jun, MIAO Nan-Sheng   

  1. 1Vegetable and Flower Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;2The Key Laboratory of Oil Crop,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;3 College of Agriculture,Jiangxi Agricultural University,Nanchang 330045,China
  • Online:2015-05-25 Published:2015-05-25

摘要: 以一年生辣椒(Capsicum annuum)材料‘V06C1720’为母本,灌木辣椒(C. frutescens)材料‘H101’为父本,建立包含180个单株的种间F2作图群体,应用SSR和SRAP标记技术构建了共278个标记位点的17个连锁群遗传图谱,图谱全长1 282.10 cM,标记平均间距为4.61 cM。利用QTLNetwork 2.0软件和 F2、F2:3表型数据,对辣椒15个主要农艺性状进行了QTL分析,在第1、2、3、4、6、7、10、12、13、14、15和16连锁群上共检测到48个加性QTL和11对上位性QTL,可分别解释5.18% ~ 40.33%和4.09% ~ 13.56%的表型变异。变异率大于10.00%的主效加性QTL有33个,占总数的68.75%;来自灌木辣椒‘H101’的增效等位加性QTL位点有29个,占总数的60.42%。株高、主茎高、果长、果径、单果质量、果形和果实辣味等7个性状的9个加性QTL在两个群体中同时被检出,这些在不同环境及不同遗传背景下能够稳定存在的QTL可为辣椒农艺性状分子标记辅助选择育种、QTL精细定位及克隆提供有价值的参考。

关键词: 辣椒, F2群体, F2:3群体, 农艺性状, QTL

Abstract: In this study,a population of F2 generation including 180 individual plants was derived from the interspecific cross between the female line Capsicum annuum‘V06C1720’and the male line C. frutescens‘H101’. A genetic linkage map containing 17 linkage groups(LGs)was constructed included 278 markers of SSR and SRAP,and covered 1 282.10 cM with an average marker interval of 4.61 cM. With the phenotypic data of F2 and F2:3 populations,QTLs for 15 main agronomic traits were analyzed by the software of QTLNetwork 2.0 in pepper. A total of 48 additive QTLs and 11 pairs of epistatic QTLs were detected,which located on LG 1,2,3,4,6,7,10,12,13,14,15 and 16,explaining5.18%–40.33% and 4.09%–13.56% of the phenotypic variation,respectively. Thirty-three additive QTLs(68.75%)were major QTLs accounting for more than 10.00% of the phenotypic variation. Twenty-nine additive QTLs alleles(60.42%)from the parent‘H101’toward increasing the trait value were detected. Nine additive QTLs controlling plant height,primary axis length,fruit length,fruit diameter,fruit weight,fruit shape and fruit pungency respectively was persistently expressed in both of F2 and F2:3 populations. The QTL that were consistent in different environments or genetic backgrounds could be useful for marker-assisted selection of agronomic traits,fine mapping and positional cloning.

Key words: pepper, F2 population, F2:3 population, agronomic trait, QTL