Overexpression of SbRFP1 Gene Improves Salt Tolerance of in Vitro Potato Plantlets

doi:10.16420/j.issn.0513-353x.2019-0769

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (2): 381-389.doi: 10.16420/j.issn.0513-353x.2019-0769

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Overexpression of SbRFP1 Gene Improves Salt Tolerance of in Vitro Potato Plantlets

ZHAO Xijuan1,2，ZHANG Huiling1,*，HOU Juan2,3，ZHANG Juping1，CHEN Sudan1，and SONG Botao2

1College of Forestry，Henan University of Science and Technology，Luoyang，Henan 471000，China；2 College of Horticulture and Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China；3 College of Horticulture，Henan Agricultural University，Zhengzhou 450002，China

Online:2020-02-25 Published:2020-02-25

Abstract

Abstract: The in vitro plantlets of transgenic lines overexpressed SbRFP1 and control were treated with NaCl at different concentrations. Results showed that the plant height，root length and fresh weight of transgenic lines were significantly higher than those of control under 5 g · L-1 or 8 g · L-1 NaCl stress. Transcriptome sequencing of transgenic lines and non-transgenic control tubers displayed that the expression levels of F-box gene（PGSC0003DMG400013116）and GDSL（Gly-Asp-Ser-Leu）gene（PGSC0003DMG400007815）in transgenic lines were significantly higher than those in control，as well as up-regulated by NaCl. The in vitro plantlets of E3（non-transgenic）were treated with exogenous substances and the expression of differentially expressed genes were detected. Results showed that the differentially expressed genes were induced by SA and other hormones，as well as NaCl. It is suggested that SbRFP1 may improve the salt tolerance of potato by regulating the expression of stress-related genes such as F-box gene and GDSL gene.

Key words: potato, SbRFP1, NaCl stress, expression analysis

CLC Number:

S 532

ZHAO Xijuan1,2，ZHANG Huiling1,*，HOU Juan2,3，ZHANG Juping1，CHEN Sudan1，and SONG Botao2. Overexpression of SbRFP1 Gene Improves Salt Tolerance of in Vitro Potato Plantlets[J]. ACTA HORTICULTURAE SINICA, 2020, 47(2): 381-389.

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Overexpression of SbRFP1 Gene Improves Salt Tolerance of in Vitro Potato Plantlets

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