Identificate Intergenic Region Deletion of Tomato yellow leaf curl virus Isolated from Beijing

doi:10.16420/j.issn.0513-353x.2015-0058

ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (8): 1591-1598.doi: 10.16420/j.issn.0513-353x.2015-0058

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Identificate Intergenic Region Deletion of Tomato yellow leaf curl virus Isolated from Beijing

ZHAO Yu-Nan, CHENG Xiao-Long, ZHAO Ping-Ping, CHEN Ya-Ping, LU Jia, LI Chang-Bao, YAO Lei

1School of Electrical Engineering，Shenyang University of Technology，Shenyang 110870，China；2Beijing Agro- Biotechnology Research Center，Beijing Academy of Agriculture and Forestry，Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology，Beijing 100097，China；3Beijing Vegetable Research Center，Beijing Academy of Agriculture and Forestry，Beijing 100097，China

Online:2015-08-25 Published:2015-08-25

Abstract

Abstract: Two distinct pathogenicity Tomato yellow leaf curl virus isolates from the greenhouse of Beijing were observed，termed BJ03 and BJ04 respectively. The full long genomic sequences of these two TYLCV isolates were sequenced；TYLCV-BJ03 is 2 781 bp，and TYLCV-BJ04 is 2 740 bp. The molecule evolution reveals that these two isolates belong to two different Chinese group of a recent evolution branch of Israel strain TYLCY-IL. They shared 99.15% nt identity. The main difference between these two isolates is the intergenic region（IR）. The BJ04 isolate contained a 41-nt deletion in the intergenic region betweenthe TATA-box and the nonanucleotide motif. This IR deletion mutant of TYLCY was confirmed by specific primers designed for the deleted region. In the further inspections，the single infected and combine infected samples were detected.

Key words: tomato, Tomato yellow leaf curl virus, intergenic region, sequence deletion

CLC Number:

S 641.2

ZHAO Yu-Nan, CHENG Xiao-Long, ZHAO Ping-Ping, CHEN Ya-Ping, LU Jia, LI Chang-Bao, YAO Lei. Identificate Intergenic Region Deletion of Tomato yellow leaf curl virus Isolated from Beijing[J]. ACTA HORTICULTURAE SINICA, 2015, 42(8): 1591-1598.

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Identificate Intergenic Region Deletion of Tomato yellow leaf curl virus Isolated from Beijing

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