Abstract: The F2 segregation population constructed by hybridization among female and male individuals of‘Winter Green 1’was our experimental material. We obtained the molecular markers linked with the sex gene X/Y using SRAP-BSA technique. Three SRAP markers（SRAP4.3，SRAP5.7，SRAP9.5）closely linked with the sex gene X/Y were gained from 256 SRAP primer combinations. SRAP5.7 and SRAP9.5 were successfully converted into SCAR markers（S5.7，S9.5）. SRAP4.3 was smoothly transformed into dCAPs marker（D4.3）. S5.7 and S9.5 marker were co-segregated with Y gene. D4.3 marker was closely linked to X/Y gene，and the genetic distance is 0.3 cM. The sex in other 7 spinach populations was identified by the marker S5.7，S9.5 and D4.3. The results of molecular identification were consistent with the phenotype，so these three molecular markers could be used for marker-assisted selection breeding（MAS）.

Key words: spinach, sex, SRAP, BSA, SCAR, dCAPs, MAS