关键词: 龙眼, ELF4, 花芽分化, 基因克隆, 基因表达

Abstract:

The full length of cDNA sequences of two ELF4 homolog genes were cloned from Dimocarpus longan‘Red Seed’，named DlELF4-1 and DlELF4-2. The functions of these two genes were analyzed by bioinformatics analysis and real-time quantitative PCR. To further study the function of the DlELF4 genes，DlELF4-1 and DlELF4-2 were overexpressed in Arabidopsis thaliana. Sequence analysis and cluster analysis showed that DlELF4-2 was similar to AtELF4-L1，while DlELF4-1 and AtELF4 showed higher similarity. Overexpression of two DlELF4 genes separately in Arabidopsis thaliana resulted in delaying flowering and adventitious roots phenotypes，suggesting that DlELF4 genes may be repressors of flowering and promoting auxin synthesis. However，expression patterns of DlELF4-1 and DlELF4-2 genes were different during bud differentiation of longan，indicating that the two genes may have different functions in longan.

Key words: longan, ELF4, flowering bud differentiation, gene cloning, gene expression