http://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
http://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
http://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
http://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
http://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2015, Vol. 42 ›› Issue (11): 2174-2182.doi: 10.16420/j.issn.0513-353x.2014-1074

• 蔬菜 • 上一篇    下一篇

黄瓜未受精胚珠离体培养及单倍体植株再生

王 烨,顾兴芳*,张圣平,苗晗   

  1. 中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2015-11-25 发布日期:2015-11-25
  • 基金资助:

    国家现代农业产业技术体系建设专项资金项目(CARS-25);农业部园艺作物生物学与种质创制重点实验室项目

Studies on Haploid Plant Induction via in vitro Unfertilized Ovule Culture#br# of Cucumber

WANG Ye,GU Xing-fang*,ZHANG Sheng-ping,and MIAO Han   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2015-11-25 Published:2015-11-25

摘要:

以欧亚杂交类型黄瓜(Cucumis sativus L.)65G × 228 的F1 为材料,对未受精胚珠进行离体
培养,通过6 因素5 水平的正交试验,研究预处理温度、诱导培养基、TDZ、KT 和BA 浓度对未受精胚
珠雌核发育的影响。结果证明TDZ 浓度对胚发生频率有极显著影响,是诱导黄瓜离体雌核发育的最主要
因素,当其浓度为0.02 mg · L-1 时胚状体的诱导率最高。诱导培养基和KT 浓度显著影响胚发生率,而预
处理温度和BA 浓度影响不显著。硝态氮(NO3
-)和铵态氮(NH4
+)含量比例均接近1∶1 的诱导培养基,
较其他比值较大的培养基的胚发生率有显著提高。利用流式细胞仪进行倍性鉴定,再生植株中单倍体率
为18.2%。

关键词: 黄瓜, 未受精胚珠, 单倍体, 流式细胞仪

Abstract:

The unpollinated ovules of cucumber(Cucumis sativus L.)line 65G × 228 were used to
obtain haploid plants via in vitro gynogenesis. With the unfertilized ovules,a regeneration system of
cucumber was established by analizing the effects of pretreatment temperature,induction medium,
concentration of TDZ,KT and BA on haploid induction. The results indicated that 0.02 mg · L-1 TDZ was
better for the initiation of gynogenesis. Induction medium and concentration of KT significantly affected
the frequency of embryo induction. Further,the highest embryo regeneration frequency was achieved at
IM3 medium supplemented with 0.75 mg · L-1 KT and 2.0 mg · L-1 BA. The embryos could grow root and
develop into the complete plant on MS medium. Flow cytometry analysis showed that 18.2% of the
regenerated plants were haploid.

Key words: cucumber, unfertilized ovule, haploid, flow cytometry

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