关键词: 杧果, 基因表达谱, RNA-Seq, 1-MCP, 采后

Abstract:

1-methylcyclopropene（1-MCP）is usually applied to extend shelf life of mango（Mangifera indica Linn‘Tainong 1’）fruits and reduce post-harvest deterioration. In order to explore the influence of 1-MCP on the fruit storage，the harvested Tainong 1 mango fruits were treated with 1-MCP（1 μL · L-1）under room temperature for 12 hours，fruits without 1-MCP treatment served as the control. Transcriptome profiling of mature mango fruits was conducted by digital gene expression（DGE）sequencing method. Seven thousand three hundred and fifty differentially expressed genes（DEGs）were identified between 1-MCP treated and control fruits. The DEGs involved in many pathways including cell wall metabolism，hormone regulation，stress and defense，oxidative damage protection，energy and metabolism，protein folding，programmed cell death mediated by the ubiquitination and proteosome pathways，as well as ripening and senescence processes. To validate the DGE data，six genes were selected for real-timequantitative PCR（qRT-PCR）analysis. Gene ontology（GO）term enrichment analysis of DEGs revealed that 1-MCP treatment could protect fruit cells by enhancing the stress resistance，inhibiting substance and energy metabolism and reducing the intracellular calcium loss. The qRT-PCR analysis confirmed the accuracy of DGE results.

Key words: Mangifera indica, gene expression profile, RNA-Seq, 1-MCP, postharvest