https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2015, Vol. 42 ›› Issue (6): 1040-1048.doi: doi:10.16420/j.issn.0513-353x.2014-1115

• 果树 • 上一篇    下一篇

桃根系与南方根结线虫早期互作的组织病理学研究

李海炎,王力荣,曹珂,朱更瑞,王新卫,方伟超,陈昌文,王小丽   

  1. 中国农业科学院郑州果树研究所,郑州 450009
  • 出版日期:2015-06-25 发布日期:2015-06-25
  • 基金资助:
    农业部农业行业专项(201303093);国家科技支撑计划项目(2013BAD01B04-19);河南科技创新人才项目

Histopathological of the Early Interactions Between Peach Roots and Meloidogyne incognita

LI Hai-Yan, WANG Li-Rong, CAO Ke, ZHU Geng-Rui, WANG Xin-Wei, FANG Wei-Chao, CHEN Chang-Wen, WANG Xiao-Li   

  1. Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China
  • Online:2015-06-25 Published:2015-06-25

摘要: 为研究桃根系与根结线虫互作的组织结构变化及抗病机理,对抗病种质‘白根甘肃桃1号’(Prunus kansuensis‘Baigen Gansu Tao 1’)和感病种质‘贝蕾’(P. persica‘Bailey’)实生幼苗进行室内人工接种南方根结线虫(Meloidogyne incognita),并对接种后6、12、18、24、30、36、42和48 h的根尖进行染色观察及接种后0、6、12、36、60和84 h的根尖组织结构变化进行石蜡切片观察。染色结果表明,根结线虫侵染‘白根甘肃桃1号’和‘贝蕾’的规律基本一致:在接种12 h后开始侵入,接种12 ~ 30 h线虫数量逐渐增加,然后线虫沿维管束向上移动,根尖线虫数量趋于减少。但与‘贝蕾’相比,‘白根甘肃桃1号’的根结线虫侵染数量显著降低,在接种30 h时仅为‘贝蕾’线虫侵染数的28%。石蜡切片结果显示‘白根甘肃桃1号’根结构受破坏程度明显较低,侵入的线虫主要聚集在一定区域,且在侵染84 h时线虫聚集区域周围的细胞出现坏死反应。

关键词: 桃, 南方根结线虫, 根系, 解剖结构, 抗性机制

Abstract: To study the changes in tissue structures and resistance mechanisms that occur due to the interaction between peach roots and root knot nematodes,seedlings of the resistant Baigen Gansu Tao 1 (Prunus kansuensis)and the susceptible Bailey(P. persica)were inoculated with Meloidogyne incognita using artificial indoor inoculation. Root tips were observed by staining at 6,12,18,24,30,36,42 and 48 h after inoculation,and the tissue structure of the root tips was observed by paraffin section at 0,6,12,36,60 and 84 h after inoculation. Staining results showed that the infection law of Baigen Gansu Tao 1 and Bailey were roughly similar:Root knot nematodes began invading at 12 h after inoculation,and the number of root knot nematodes increased gradually during the period between 12 h to 30 h after inoculation. The number of root knot nematodes present in the roots subsequently decreased as theorganisms began their upward movement along the plants’ vascular bundles. Compared to Bailey,at 30 h after inoculation Baigen Gansu Tao 1 were infected with root knot nematodes at a significantly lower rate:Only 28% of that of Bailey. Paraffin section results showed that the structural damage to inoculated Baigen Gansu Tao 1 was much less severe than in inoculated Bailey. Nematodes gathered in certain areas,which led to cells necrosis at the gathering sites at 84 h after inoculation.

Key words: peach, Meloidogyne incognita, root, anatomical structure, resistance mechanism

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