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园艺学报 ›› 2015, Vol. 42 ›› Issue (2): 243-251.doi: 10.16420/j.issn.0513-353x.2014-0898

• 果树 • 上一篇    下一篇

桃生长素酰胺水解酶基因家族序列特征及其表达分析

潘磊,曾文芳,牛良,鲁振华,崔国朝,王志强   

  1. 中国农业科学院郑州果树研究所,国家桃葡萄改良中心,农业部果树育种技术重点实验室,郑州 450009
  • 出版日期:2015-02-25 发布日期:2015-02-25
  • 基金资助:

    国家‘863’计划项目(2011AA10020606);国家科技支撑计划项目(2013BAD02B03-2)

Sequence Characteristics and Expression Analysis of IAA-Leucine Resistant1-like Hydrolase Genes in Peach

PAN Lei,ZENG Wen-fang,NIU Liang,LU Zhen-hua,CUI Guo-chao,and WANG Zhi-qiang   

  1. Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,National Peach and Grape Improvement Center,Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture,Zhengzhou 450009,China
  • Online:2015-02-25 Published:2015-02-25

摘要: 生长素酰胺水解酶(IAA-Leucine Resistant1-like Hydrolase,ILL)可催化生长素结合态释放游离态生长素(IAA)。为了深入研究桃ILL基因家族的功能,对桃基因组中ILL基因家族成员的数量、在染色体骨架的分布、编码蛋白质的结构、基因表达模式和编码氨基酸的进化树分类等方面进行了研究。桃基因组中共鉴定出了9个ILL基因,它们分布在4个染色体骨架上(scaffold 1、2、3和7)。在桃中编码ILL蛋白家族的基因成员分别为:PpILR1、PpILR2、PpILR3、PpILR4、PpILL1、PpILL2、PpILL3、PpILL4和PpILL5,所有这些预测的蛋白都含有M20结构域和M20二聚化结构域。运用荧光定量实时PCR技术对该基因家族在桃果实生长发育各阶段的表达水平进行了分析,结果显示:ILL 基因家族中的数个成员在果实不同发育阶段表达水平有变化,其中PpILR1的表达量在果实生理成熟期明显上调。

关键词: 桃, 果实发育, 生长素酰胺水解酶, 生长素, 溶质型

Abstract: The IAA-Leucine Resistant1-like Hydrolase(ILL)genes is thought to regulate the release of free indole-3-acetic-acid(IAA)which is involved in the regulation of fruit development and others developmental processes. In order to further study the function of ILL family genes in peach,the member number of ILL gene family was confirmed;Thereafter their distribution on the scaffold,putative proteins,phylogenetic classification,and expression patterns were analyzed. We identified 9 ILL genes in the entire peach genome,which distributed across 4 scaffolds(1,2,3 and 7). The members of the ILR1-like family genes were PpILR1,PpILR2,PpILR3,PpILR4,PpILL1,PpILL2,PpILL3,PpILL4 and PpILL5. All of their putative proteins contained M20 and M20 dimerisation domains. The relative expression levels in mesocarps of peach fruit at different developmental stages were also detected by quantitative real-time PCR. The results showed that several genes exhibited different expression patterns during fruit development.Moreover,the transcript level of PpILR1 was significantly up-regulated at the late-ripening stage.

Key words: Prunus persica, fruit development, IAA-Leucine Resistant1-like Hydrolase, indole-3- acetic-acid, melting-flesh

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