与黄瓜矮生基因连锁的ISSR标记及其SCAR转换

园艺学报 ›› 2008, Vol. 35 ›› Issue (11): 1627-1634.

与黄瓜矮生基因连锁的ISSR标记及其SCAR转换

嵇怡¹,徐强¹,缪旻珉¹,梁国华²,陈学好^1*

（¹扬州大学园艺与植物保护学院，江苏扬州 225009；²扬州大学教育部植物功能基因组重点实验室，江苏扬州 225009）

收稿日期:2008-05-05 修回日期:2008-10-10 出版日期:2008-11-25 发布日期:2008-11-25
通讯作者: 陈学好

Molecular Tagging of ISSR and Conversion of SCAR Maker Linked to a Mutant Dwarf Gene in Cucumber

JI Yi¹;XU Qiang¹;MIAO Ming-ming¹;LIANG Guo-hua²;and CHEN Xue-hao^1*

(¹School of Horticulture and Plant Protection,YangzhouUniversity,Yangzhou,Jiangsu 225009,China;²Key Laboratory of Education Ministry for the Plant Function Genomics, Yangzhou University , Yangzhou,Jiangsu 225009,China)

Received:2008-05-05 Revised:2008-10-10 Online:2008-11-25 Published:2008-11-25
Contact: CHEN Xue-hao

摘要/Abstract

摘要：

以黄瓜矮生品系D8与蔓生品系JIN5杂交并自交得到的124株F₂代为试材，应用ISSR分子标记技术和BSA（混合群体分组分析法）法寻找与黄瓜矮生基因连锁的分子标记。从80条ISSR引物中筛选到一个黄瓜矮生性状特异的多态性引物UBC818，经F₂代单株验证，UBC818的扩增片段在蔓生黄瓜JIN5植株中稳定出现，而在矮生黄瓜D8植株中没有。连锁关系分析表明，标记与黄瓜矮生基因间的遗传距离为11.1 cM。根据对该片段的序列分析结果重新设计了1对引物，将ISSR标记成功转化成了SCAR标记，并命名为UBC818-S。

关键词: 黄瓜, 矮生基因, ISSR, SCAR

Abstract: In this study, 124 of F₂ plants from the cross combination of the dwarf cucumber D8 and the vine plant JIN5 were used to screen the molecular marker linked to the dwarf mutant gene in cucumber with ISSR molecular tagging and BSA (bulked segregation analysis) method. UBC818, a specific primer of polymorphism, was obtained from eighty ISSR primers screening for a cucumber dwarf traits specific in the dwarf line. The marker from the results of PCR with UBC818 was tested in individual DNAs reaction for the F₂ population and the UBC818 amplified fragment could only be detected in the vine plants. Linkage analysis showed that the genetic distance between the specific marker and the cucumber dwarf mutant gene was 11.1 cM. According to the sequence of the fragment analysis, one pair of primers has been designed. The ISSR marker was successfully converted into a SCAR marker which was named UBC818-S.

Key words: cucumber, dwarf mutant gene, ISSR, SCAR

中图分类号:

S 642.2

嵇怡;徐强;缪旻珉;梁国华;陈学好. 与黄瓜矮生基因连锁的ISSR标记及其SCAR转换[J]. 园艺学报, 2008, 35(11): 1627-1634.

JI Yi;XU Qiang;MIAO Ming-ming;LIANG Guo-hua;and CHEN Xue-hao. Molecular Tagging of ISSR and Conversion of SCAR Maker Linked to a Mutant Dwarf Gene in Cucumber [J]. ACTA HORTICULTURAE SINICA, 2008, 35(11): 1627-1634.

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