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园艺学报 ›› 2011, Vol. 38 ›› Issue (5): 977-984.

• 研究报告 • 上一篇    下一篇

马蔺与溪荪种间杂交亲和性研究

毕晓颖1,2,* ,娄 琦1,郑 洋1,2   

  1. 1 沈阳农业大学园艺学院,沈阳 110866;2 辽宁省北方园林植物与地域景观高校重点实验室,沈阳 110866)
  • 收稿日期:2010-01-18 修回日期:2011-04-12 出版日期:2011-05-25 发布日期:2011-05-25
  • 通讯作者: 毕晓颖

Studies on Compatibility of Interspecific Hybridization Between Iris lactea Pall. var. chinensis Koidz. and I. sanguinea Hormen.

BI Xiao-ying 1,2,* ,LOU Qi 1,and ZHENG Yang 1,2   

  1. 1 College of Horticulture,Shenyang Agricultural University,Shenyang 110866,China;2 Key Laboratory of Northern Landscape Plants and Regional Landscape,Shenyang 110866,China)
  • Received:2010-01-18 Revised:2011-04-12 Online:2011-05-25 Published:2011-05-25
  • Contact: BI Xiao-ying

摘要: 以鸢尾属植物马蔺(Iris lactea Pall. var. chinensis Koidz.)和溪荪(Iris sanguinea Hormen.)为试材,对常规授粉后结实性及花粉管的行为进行了观察,并研究不同授粉方式对马蔺和溪荪正反交结实率的影响,探讨了其种间杂交的亲和性。结果表明:马蔺与溪荪杂交表现不亲和性,常规授粉正反交结实率均为0;常规授粉2 h后,溪荪的花粉在马蔺的柱头上能萌发,但花粉粒萌发和花粉管的伸长较两亲本自交滞后,柱头和通道内胼胝质现象明显;马蔺的花粉在溪荪的柱头上不能萌发。几种不同授粉方式处理中,马蔺 × 溪荪组合最适授粉方式为0.1% 6-BA处理柱头后授粉,结实率和有胚率分别为10%和16.67%。溪荪 × 马蔺组合的最适授粉方式为提前授粉,结实率和有胚率分别为15%和25.70%。

关键词: 鸢尾属, 种间杂交, 亲和性, 授粉方式, 荧光

Abstract: The cross-compatibility between two Iris species,Iris lactea Pall. var. chinensis Koidz. and I. sanguinea Hormen. was studied by capsule-setting and pollen tube behaviors after common pollination,so was the effects of different pollination methods on fruiting rate. The results showed that no capsules were obtained from reciprocal crosses between the two species,which inferred cross-incompatibitily between them. Pollen grains of I. sanguinea germinated at stigma of I. lactea var. chinensis after 2 hours of pollination,significantly lagging behind self-fertilization of parents,no germination of I. lactea var. chinensis pollen grains was observed on stigma of I. sanguinea. The optimum pollination method for I. lactea var. chinensis × I. sanguinea was 0.1% 6-BA treatment before pollination,fruiting rate and embryo rate reached to 10% and 16.67%,respectively,while the benefit way for I. sanguinea × I. lactea var. chinensis was pollination in advance,fruiting rate was arrival at 15%,and embryo rate was 25.70%,respectively.

Key words: Iris L., interspecific hybridization, compatibility, pollination method, fluorescence

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