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园艺学报 ›› 2002, Vol. 29 ›› Issue (6): 588-589.

• 研究报告 • 上一篇    下一篇

‘美人’梅与其近缘种亲缘关系的AFLP研究

明 军1;张启翔2;毛庆山3;晏小兰3
  

  1. (1 湖北农学院园艺系, 荆州434025 ; 2 北京林业大学园林学院, 北京100083 ; 3 中国梅花中心, 武汉430000)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-12-25 发布日期:2002-12-25

AFLP Analysis of Genetic Relationships of Prunus mume ‘Meiren’and Relatives

Ming Jun1, Zhang Qixiang2, Mao Qingshan3, and Yan Xiaolan3
  

  1. (1 Department of Horticulture , Hubei Agricultural College , Jingzhou 434025 , China ; 2 Beijing Forestry University , Beijing 100083 , China ; 3 The Research Center of China Mei Flower , Wuhan 430000 , China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-12-25 Published:2002-12-25

摘要: 以美人梅型的4 个品种以及部分近缘种为试材, 运用AFLP 银染法, 采用引物组合E-ACT/M-
CAT, E-GA/M-CTC , E-ACT/M-CCA , E-ACT/M-CAC , 扩增得到420 个位点的6 300 条带数据。使用NTSYSpc 2. 1 t 软件, 分别采用DICE , SM和Jaccard 匹配系数, SAHN Clustering 进行不加权成对算术平均法(UPGMA) 聚类分析, 得到了‘美人’梅与近缘种的亲缘关系依次为梅、紫叶李、李、山桃、桃花、山杏和杏的结果, 与形态学、遗传育种学的相关结论相符。

关键词: 梅, 美人梅, 亲缘关系, AFLP

Abstract: The silver-stained AFLP analysis was employed to analyse the DNA fingerprints of four cultivars of Meiren Mei Form , and a wild species , a Pink Double Form and seven relatives. For 420 loci 6 300 bandings was
capable of producing sharp fingerprint patterns with the primer combinations of E-ACT/M-CAT , E-GA/M-CTC , E-ACT/M-CCA , E-ACT/M-CAC. NTSYSpc 2. 1 was used to SAHN Clustering of UPGMA and according to DICE coefficient , Jaccard coefficient and Simple Matching coefficient (SM) , cluster analysis was done. The relationship among the species was discussed. The result indicated that Prunus mume‘Meiren’was the most close to Prunus
mume and Prunus cerasifera‘Pissardii’, and distantly related to plum , peach and apricot , conforming to morphological classification and breeding record.

Key words: Prunus mume Sieb. et Zucc., Prunus mume ‘Meiren’, Genetic relationships, Amplified Fragment Length Polymorphism

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