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园艺学报 ›› 2005, Vol. 32 ›› Issue (3): 433-437.

• 研究论文 • 上一篇    下一篇

低氧胁迫下黄瓜幼苗根系多胺代谢的变化

汪 天1,2;王素平1;郭世荣1;高洪波1   

  1. (1 南京农业大学园艺学院, 南京210095; 2 安徽农业大学森林利用学院, 合肥230036)
  • 收稿日期:2004-07-29 修回日期:2004-11-15 出版日期:2005-06-25 发布日期:2005-06-25

Changes of Polyamines Metabolism in Roots of Cucumber Seedlings underRoot-zone Hypoxia Stress

Wang Tian1, 2;Wang Suping1;Guo Shirong1;Gao Hongbo1   

  1. (1College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; 2College of Forest Utilization, Anhui Agricultural University, Hefei 230036, China)
  • Received:2004-07-29 Revised:2004-11-15 Online:2005-06-25 Published:2005-06-25

摘要: 利用外源Spd、PAO 抑制剂研究了黄瓜幼苗根系在低氧水培时的生长状况以及内源PAs、
H2O2 含量和PAO活性的动态变化。结果表明, 外源PAs对低氧胁迫下黄瓜幼苗根系的生长有促进作用;低氧处理后3种PAs含量都明显上升, 外源Spd和PAO抑制剂增加了根系中的PAs含量, 降低了根系中H2O2 含量, 减缓了低氧伤害; PAs为低氧胁迫的应激产物, PAO为适应酶类, 其活性随PAs含量的变化而发生变化。

关键词: 黄瓜, 低氧胁迫, 多胺, H2O2, 多胺氧化酶

Abstract: The effects of exogenous spermidine (Spd) and inhibitor of polyamine oxidase (PAO) on growth of roots, endogenous polyamine levels, hydrogen peroxide (H2O2 ) content and activity of polyamine oxidase in roots of cucumber seedlings under root-zone hypoxia grown hydroponicallywere studied. The results showed that exogenous polyamines could imp rove roots growth of cucumber seedlings, and levels of free putrescine Put) , spermidine (Spd) and spermine (Spm) in cucumber roots obviously increased under hypoxia stress. Exogenous Spd and inhibitor of PAO induced an increase in free polyamine levels and decreased H2O2 content in cucumber roots, which could alleviate damage of hypoxia stress to cucumber seedlings. Polyamines are products responding to hypoxia stress and PAO belongs to adaptive enzyme whose activities change with levels of polyamines.

Key words: Cucumber, Hypoxia stress, Polyamine, Hydrogen peroxide, Polyamine oxidase

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