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园艺学报 ›› 2011, Vol. 38 ›› Issue (2): 311-311–316.

• 观赏植物 • 上一篇    下一篇

乙烯和1-MCP的竞争性作用对月季花器官乙烯生成量及相关基因表达的影响

薛璟祺1,杨 丰2,左志锐3,高俊平2,*   

  1. (1中国农业科学院蔬菜花卉研究所,北京 100081;2中国农业大学观赏园艺与园林系,北京 100193;3江苏省花卉种质创新工程技术研究中心,江苏苏州 215600)
  • 收稿日期:2010-11-17 修回日期:2011-01-25 出版日期:2011-02-25 发布日期:2011-02-25
  • 通讯作者: 高俊平

Effect of Ethylene and 1-MCP on Ethylene Production and Related Gene Expression in Floral Tissues of Roses

XUE Jing-qi1,Yang Feng2,ZUO Zhi-rui3,and GAO Jun-ping2,*   

  1. (1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Department of Ornamental Horticulture and Landscape Architecture,Beijing 100193,China;3Engneering Research Center for Floriculture,Suzhou,Jiangsu 215600,China)
  • Received:2010-11-17 Revised:2011-01-25 Online:2011-02-25 Published:2011-02-25
  • Contact: GAO Jun-ping

摘要: 以探讨月季切花不同花器官的乙烯生物合成和对乙烯的感受为目的,以切花月季‘Samantha’为试材,进行了乙烯和1-MCP交叉处理,测定花朵各器官乙烯生成量,Rh-ACS3、Rh-ACO1和Rh-ETR3基因的表达。结果表明,1-MCP前处理或后处理均能有效抑制花瓣、雌蕊和花托中由于外源乙烯造成的内源乙烯生成量增加。在基因表达方面,Rh-ACS3、Rh-ACO1和Rh-ETR3在花瓣和雌蕊中对乙烯处理的诱导表达均可被1-MCP前处理或后处理有效抑制,而在花托中,这种抑制主要针对Rh-ETR3。这些结果说明,1-MCP对乙烯的竞争性抑制作用主要发生在花瓣、雌蕊和花托上,其竞争效果在乙烯处理前或乙烯处理后均有效。

关键词: 月季, 切花, 乙烯, 1-MCP, 基因表达

Abstract: The effects of ethylene and its inhibitor,1-methylcyclopropene(1-MCP)treatments on the flower opening,ethylene production,and the expression of ethylene biosynthesis- and perception-related genes in cut rose‘Samantha’were investigated. The results showed that 1-MCP pre-treatment or post-treatment substantially inhibited ethylene-caused increase of ethylene production in petals,gynoecia,and receptacles. 1-MCP treatments also substantially inhibited the expression of Rh-ACS3,Rh-ACO1 and Rh-ETR3 genes in petals and gynoecia,but of only Rh-ETR3 in receptacles. The results indicated that the effect of competitive inhibition of 1-MCP on ethylene occurred mainly in petals,gynoecia and receptacles,and the effect was substantial,regardless the treatment was performed before or after ethylene treatment.

Key words: cut rose, ethylene, 1-MCP, gene expression

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