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园艺学报 ›› 2010, Vol. 37 ›› Issue (1): 23-30.

• 果树 • 上一篇    下一篇

普通杏品种SSR遗传多样性分析

张淑青1;刘冬成2;刘威生3 ;张爱民2 ; 李绍华1*   

  1. (1 中国科学院植物研究所, 北京100093; 2 中国科学院遗传与发育生物学研究所, 北京100101; 3 辽宁省果树科学研究所, 辽宁熊岳115009)
  • 收稿日期:2009-05-13 修回日期:2009-11-12 出版日期:2010-01-25 发布日期:2010-01-25
  • 通讯作者: 李绍华

Analysis of Genetic Diversities in Apricot Cultivars ( Prunus armeniaca L. ) with Simple Sequence Repeat ( SSR) Markers

ZHANG Shu-qing1 ; L IU Dong-cheng2 ;L IU Wei-sheng3 ;ZHANG Ai-min2 ;and LI Shao-hua1*   

  1. (1 Institute of Botany, the Chinese Academy of Sciences, Beijing 100093,China; 2 Institute of Genetics and Developmental Biology,the Chinese Academy of Sciences, Beijing 100101, China; 3Liaoning Institute of Pomology, Xiongyue, L iaoning 115009, China)
  • Received:2009-05-13 Revised:2009-11-12 Online:2010-01-25 Published:2010-01-25
  • Contact: LI Shao-hua

摘要: 利用来自杏、桃和扁桃的25对SSR多态性引物对66个普通杏品种进行了PCR扩增及变性聚丙烯酰胺凝胶电泳, 共检测出284个等位位点, 每对引物的等位位点数在3~17之间, 平均11136。通过NTSYS软件计算得到的Dice相似性系数为01083~01987, 平均值为01370, 表明中国普通杏种质资源的遗传多样性丰富。UPGMA法聚类将66份材料分为5个组。SSR标记反映出的品种间亲缘关系与根据地理生态类型对杏种质的分类结果基本一致。来自四川和贵州的多数品种独立聚成一组, 表现出与其它品种较远的亲缘关系, 该地区可能存在特异性较高的种质资源。证实了扁桃基因组SSR引物可用在杏SSR标记的研究中。

关键词: 杏, SSR, 遗传多样性

Abstract: Twenty-five pairs of SSR primers from apricot, peach and almond were used to detect the genetic diversities in 6 apricot ( Prunus armeniaca L. ) varieties. The results showed that 284 alleles were detected by these primers, and 3 to 17 alleles could be detected with an average of 11.36 alleles for each primer. Revealed by NTSYS, the genetic similarity varied from 0.083 to 0.987, with an average of 0.370,which showed that the genetic diversity of Chinese apricot germplasm was abundant. The clustering results of UPGMA showed that the 66 varieties were clustered into 5 groups, which were basically consistent with traditional geographical classification. Most cultivars from Sichuan and Guizhou Province were clustered into one group which was far from other varieties, and indicates that there may be some special germplasm in this area. Our experiment proved that SSR markers from almond genome are transferable to apricot as well.

Key words: Apricot, SSR, genetic diversity

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