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园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 1141-1144.

• 研究报告 • 上一篇    下一篇

云南野生兰属主要种间亲缘关系的AFLP分析

张俊祥1;李枝林2;范成明1;程少丽1;赵明富1;何月秋1*   

  1. (1云南农业大学植物保护学院, 云南昆明650201; 2 云南农业大学园林园艺学院, 云南昆明650201)
  • 收稿日期:2005-11-18 修回日期:2006-05-29 出版日期:2006-10-25 发布日期:2006-10-25

Phylogenetics Analysis of the Chinese Orchids in Yunnan Province Using AFLP Technique

Zhang Junxiang1;Li Zhilin2;Fan Chengming1;Cheng Shaoli1;Zhao Mingfu1;He Yueqiu1*   

  1. (1College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, China; 2College of Gardening and Horticulture, Yunnan Agricultural University, Kunming, Yunnan 650201, China)
  • Received:2005-11-18 Revised:2006-05-29 Online:2006-10-25 Published:2006-10-25

摘要: 采用AFLP技术对云南省兰属的14个种和3个变种中的38份样本进行了亲缘关系分析。16对AFLP引物扩增的条带聚类结果表明, 云南省国兰遗传多样性非常广泛, 相同品种间的不同个体扩增出的条带模式相差很大。依据AFLP数据, 在相似系数0.58的水平下, 38个兰花样品分成3组: 莲瓣兰、春剑、惠兰、寒兰分为第1组, 建兰、豆瓣兰、春兰, 墨兰、虎头兰、西藏虎头兰、大雪兰、碧玉兰、文山红柱兰、长叶兰和兔耳兰分为第2组, 送春和蜜蜂兰分为第3组。

关键词: 兰属, AFLP, 遗传多样性, 亲缘关系

Abstract: Thirty-eight collections of Chinese orchids in Yunnan Province, China, including 14 species and 3 varieties, were analyzed phylogenetically using AFLP technique. PCR amplification band pattern with 16 pairs of primers indicated that the Chinese orchids were very diverse, and individuals of the same species had different band patterns. Subgenus Jensoa appeared as a paraphyletic group. Subgenus Cyperorch is was not a monophyletic group instead formed an offshoot of subgenus Jensoa. At a similarity coefficient of 0.58, the orchids could be clustered into 3 groups: Cybidium tortisepalum Wu et Chen, C. tortisepalum var. longibracteatum, C. faberi and C. kancan belonged to Group 1; C. ensifolium, C. goeringii var. serratum, C.goeringii, C. sinense, C. hookerianum, C. tracyanum, C. mastersii, C. lowianum, C. wenshanense,C. erythraeum and C. lancifolium to Group 2; C. cyperifolium var. szechuanicum and C. pumilum to Group 3.

Key words: Cybidium, AFLP, Genetic diversity, Phylogenetic relationship