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园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 99-104.

• 蔬菜 • 上一篇    下一篇

大白菜叶色相关性状的QTL定位与分析

徐东辉1,2 ;孙日飞1 ;张延国1 ; 原玉香1,2,3 ; 康俊根4 ; 武 剑1 ; 张 慧1,2 ;宋晓飞5 ; 李晓楠1,2 ;宋一沫6 ;王晓武1*   

  1. (1 中国农业科学院蔬菜花卉研究所, 北京100081; 2 中国农业科学院研究生院, 北京100081; 3 河南农业科学院生物技术研究所, 郑州450002; 4 山西省农业科学院蔬菜研究所, 太原030031; 5 河北农业大学园艺学院, 河北保定071001; 6 首都师范大学生命科学学院, 北京100037)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Mapping and Analysis of QTL Related to Leaf Color in Chinese Cabbage( Brassica rapa L. ssp. pekinensis)

XU Dong-hui1,2;SUN Ri-fei1;ZHANG Yan-guo1;YUAN Yu-xiang1,2,3;KANG Jun-gen4;WU Jian1;ZHANG Hui1,2;SONG Xiao-fei5;LI Xiao-nan1,2;SONG Yi-mo6;WANG Xiao-wu1*   

  1. ( 1 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 The Graduate School ofChinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Institute of Biotechnology, Henan Academy of AgriculturalSciences, Zhengzhou 450002, China; 4 Institute of Vegetables, Shanxi Academ y of Agricultural Sciences, Taiyuan 030031, China;5College of Horticulture, Hebei AgriculturalUniversity, Baoding, Hebei 071001, China; 6College of L ife sciences, CapitalN orm alUniversity, Beijing 100037, China )
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要: 应用已构建了AFLP遗传图谱的大白菜DH群体, 采用多模型QTL作图的方法, 通过色差计
法、叶色素测定法和人为目测法3种方法7个指标对叶色相关性状进行QTL定位和分析。结果表明, 在7
个连锁群上共检测到18个QTL, 其中色差计法检测到9个QTL, 叶色素测定法检测到8个QTL, 人为目测
法仅检测到1个QTL。另外, 估算了单个QTL的贡献率和加性效应, 发现各QTL的加性效应各不相等, 各
位点的贡献率在5.5%~15.6%之间。

关键词: 大白菜, 叶色, 数量性状位点(QTL), 连锁群

Abstract: A Chinese cabbage (Brassica rapa L. ssp. pekinensis) double-hap loid (DH) population with a
reported AFLP genetic map was emp loyed to map quantitative trait loci (QTL ) related to leaf color by using
MAPQTL 410 package. Tristimulus colorimeter, leafy p igment determination and ocularmeasurementwere used
to measure the traits related to leaf color. A total of 18 QTL were detected on 7 linkage group s, 9, 8 and 1 QTL
were detected by 3 methods, respectively. These QTL individually exp lained 5.5% to 15.6% of the phenotyp ic
variation, and there were unequal gene effects on the traits related to leaf color. These mapped QTL could be
used to develop a marker assisted selection program for leaf color in Brassica rapa vegetable varieties

Key words: Chinese cabbage, Leaf color, QTL, Linkage group