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园艺学报 ›› 2013, Vol. 40 ›› Issue (5): 905-.

• 蔬菜 • 上一篇    下一篇

甜瓜中甜菜碱醛脱氢酶基因CmBADH的克隆及非生物胁迫下的表达分析

丛红滋, 于喜艳 , 王秀峰, 史庆华   

  1. (山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018)
  • 出版日期:2013-05-25 发布日期:2013-05-25

Cloning and Expression Analyzing of One BADH Gene CmBADH of
Muskmelon Under Abiotic Stress

CONG  Hong-Zi, YU  Xi-Yan- , WANG  Xiu-Feng, SHI  Qing-Hua   

  1. (State Key laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural
    University,Tai’an,Shandong 271018,China)
  • Online:2013-05-25 Published:2013-05-25

摘要: 以甜瓜(Cucumis melo L.)‘TS-2’为材料,根据GenBank 登录的植物甜菜碱醛脱氢酶氨基
酸保守序列设计兼并引物,利用RT-PCR 和3′、5′RACE 技术从甜瓜叶片中克隆到1 个甜菜碱醛脱氢酶基
因,命名为CmBADH,在GenBank 中的注册号为:JN091961.1。生物信息学分析表明,该基因全长1 856 bp,
编码503 个氨基酸,BADH 蛋白大小约54 kD,理论pI 为5.182。甜瓜BADH 蛋白与麻疯树同源性最高,
为82.96%。该基因编码蛋白有4 个强的跨膜螺旋结构。PlantCare 分析结果显示该基因序列具有茉莉酸甲
酯、防御与胁迫、玉米醇蛋白代谢途径、低温、光响应、分生组织表达、生理周期调控等顺式作用元件
和干旱诱导的MYB 结合位点。实时荧光定量PCR 表明,CmBADH 受盐、干旱、低温、高温诱导,其表
达均呈现先上升后下降的趋势;CmBADH 还随外源ABA 诱导时间的延长呈现上升表达的趋势。

关键词: 甜瓜, BADH 基因, 克隆, 非生物胁迫, 表达

Abstract: Abstract:The muskmelon(Cucumis melo L.)of TS-2 was used as experimental materials,using PCR
degenerate primers designed based on the conserved amino acid sequences of known betaine aldehyde
dehydrogenase to amplify cDNA fragments from muskmelon by RT-PCR and 3′,5′RACE,a BADH gene
named CmBADH was cloned and the registration number in GenBank is JN091961.1. Bioinformatics
analysis indicated that the full length sequence of BADH was 1 856 bp,encoding 503 amino acids residues
with a calculated molecular weight of 54 kD and isoelectric point of 5.182. The BADH protein in
muskmelon showed the highest similarity with from Jatropha curcas,and the similarity was 82.96%.
There were four strong inside to outside transmembrane helixes. PlantCare analysis indicated that there
were cis-acting responsive elements of MeJA-responsiveness,defense and stress,zein metabolism
regulation,low-temperature,light responsiveness,meristem expression,and circadian control as well as
MYB binding site involved in drought-inducibility. The result of RT-PCR analysis indicated that the
expression of CmBADH is induced by salt stress,drought stress,low temperature and high temperature
stress,and its expression trend increased firstly and then decreased the expression of CmBADH was also
induced by ABA treatment.

Key words: Cucumis melo L., BADH gene, cloning, abiotic stress, expression

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