http://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
http://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
http://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
http://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
http://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2020, Vol. 47 ›› Issue (10): 2027-2039.doi: 10.16420/j.issn.0513-353x.2020-0023

• 研究报告 • 上一篇    下一篇

桂花R2R3-MYB家族基因鉴定及其在花开放过程中的表达分析

姚亦凡,董 彬,冯成庸,杨丽媛,赵宏波*   

  1. 浙江省园林植物种质创新与利用重点实验室,浙江农林大学风景园林与建筑学院,浙江临安 311300
  • 出版日期:2020-10-25 发布日期:2020-11-10
  • 基金资助:
    国家自然科学基金项目(31401902,31501790);浙江省农业新品种选育重大科技专项(2016C02056-12);浙江省重点研发计划项目(2019C02036);浙江省自然科学基金项目(LQ19C160012)

Identification of the R2R3-MYB Family of Osmanthus fragrans and Its Expression in the Process of Flower Opening

YAO Yifan,DONG Bin,FENG Chengyong,YANG Liyuan,and ZHAO Hongbo*   

  1. Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants,School of Landscape Architecture,Zhejiang A & F University,Lin’an,Zhejiang 311300,China
  • Online:2020-10-25 Published:2020-11-10

摘要: 对桂花(Osmanthus fragrans)R2R3-MYB转录因子进行鉴定和生物信息分析,分析相关基因在花开放过程中的表达,获得了响应相对低温调控桂花花开放和着色等的关键基因。利用转录组数据分析得到35个桂花R2R3-MYB,这些基因均包含R2R3结构域,且高度保守。通过分析MYB基因在19 ℃(花能开放)和23 ℃(花不能开放)处理以及不同组织中的相对表达量发现,OfMYB1、OfMYB12、OfMYB14、OfMYB23、OfMYB24随着花的开放表达量逐渐升高,其中OfMYB1和OfMYB14在盛花期的花瓣中表达量最高,表明其可能参与桂花着色的过程;而OfMYB4、OfMYB5、OfMYB17、OfMYB28、OfMYB34在花开放过程中的表达量呈先升高后降低的趋势,尤其是OfMYB5、OfMYB17在花芽(圆珠期)中表达量最高,表明其可能参与了花开放过程的调控。

关键词: 桂花, MYB转录因子, 环境低温, 花开放, 基因表达

Abstract: Through the identification and bioinformatics analysis of Osmanthus fragrans,and the expression analysis of related genes during flower opening,the key genes that respond to the relatively low temperature regulation of osmanthus flower opening and coloring were obtained. Using transcriptome data to analyze osmanthus R2R3-MYB genes,a total of 35 R2R3-MYBs were obtained. These genes all contained R2R3 domains and were highly conserved. The MYB transcription factors were treated in 19 ℃(flowers can open)and 23 ℃(flowers can not open)and the relative expression of different tissues was found that OfMYB1,OfMYB12,OfMYB14,OfMYB23 and OfMYB24 with the opening of O. fragrans,the expression increased gradually. The expression of OfMYB1 and OfMYB14 was the highest among the petals in flowering stage,indicating that they might be involved in the process of osmanthus coloring. OfMYB4,OfMYB5,OfMYB17,OfMYB28,OfMYB34 expression increased first and then decreased during flower opening,which indicated that OfMYB4,OfMYB5,OfMYB17,OfMYB28 and OfMYB34 could be involved in the regulation of the process of flower opening.

Key words: Osmanthus fragrans, MYB transcription factor, ambient temperature, flower opening, gene expression

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