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园艺学报 ›› 2020, Vol. 47 ›› Issue (5): 837-852.doi: 10.16420/j.issn.0513-353x.2019-0626

• 研究论文 • 上一篇    下一篇

香蕉漆酶基因家族鉴定及低温胁迫下的表达分析

刘彦英,倪珊珊,项蕾蕾,陈裕坤,赖钟雄*   

  1. 福建农林大学园艺植物生物工程研究所,福州 350002
  • 出版日期:2020-05-25 发布日期:2020-05-25
  • 基金资助:
    国家重点研发计划项目(2019YFD1000901);国家现代农业产业技术体系建设专项资金项目(GARS-31-15);福建省高原学科建设经费项目(102/71201801101);福建农林大学科技创新专项基金项目(KF2015108,CXZX2013119,CXZX2017351,CXZX2017189,CXZX2018076)

Genome-wide Identification of the Laccase Gene Family and Its Expression Analysis Under Low Temperature Stress in Musa accuminata

LIU Yanying,NI Shanshan,XIANG Leilei,CHEN Yukun,and LAI Zhongxiong*   

  1. Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Online:2020-05-25 Published:2020-05-25

摘要: 为研究香蕉漆酶基因家族成员的功能,采用生物信息学方法鉴定香蕉漆酶基因成员,分析其低温胁迫下的表达情况,为香蕉抗寒研究提供理论依据。从香蕉A(Musa acuminata)基因组筛选了22条漆酶基因(MaLAC),从香蕉B(Musa balbisiana)基因组筛选了11条漆酶基因(MbLAC),通过生物信息分析发现MaLAC比MbLAC更保守。分子进化树分析结果表明MaLAC可分为5类,MbLAC可分为4类。启动子顺式作用元件分析结果表明,MaLAC启动子序列包含大量光响应、激素应答及非生物胁迫等应激响应元件,推测MaLAC基因家族在响应逆境胁迫的过程中发挥重要作用。利用qRT-PCR技术对MaLAC部分成员在响应不同低温胁迫的表达情况进行分析,MaLAC3-2、MaLAC4、MaLAC4-5、MaLAC17在低温(13、4、0 ℃)处理下表达量均极显著高于对照(28 ℃),推测可能在香蕉响应低温胁迫的过程中发挥重要作用。预测香蕉MaLAC家族有18个成员受miRNA调控,主要受miR397和miR408的调控;推测香蕉MaLAC可能参与miRNA调控途径。

关键词: 香蕉, 漆酶家族, 成员鉴定, 生物信息分析, 低温胁迫

Abstract: Laccase has been shown a multifunctional enzyme that plays many important roles. The whole genome-wide of identification of banana laccase family members was conducted and analyzed. Using bioinformatics approaches,22 laccase genes(MaLAC)were identified from Musa acuminata genome and 11 laccase genes(MbLAC)were also identified from Musa balbisiana genome. Most of MaLAC and MbLAC members contained three typical Cu-oxidase domains. Gene structure analysis showed that most of MaLACs and MbLACs were composed of five introns and six exons. Phylogenetic clustering analysis indicated that the MaLACs were divided into five groups and the MbLACs were divided into four groups. The promoter cis-acting elements showed that the MaLAC promoter sequence contained a large number of stress response elements,such as light response,hormone response and abiotic stress,etc. The results of qRT-PCR analysis showed that the expression of some members of MaLACs increased under low temperature stress. The expression of MaLAC3-2,MaLAC4,MaLAC4-5,and MaLAC17 increased significantly at low temperatures of 13 ℃,4 ℃ and 0 ℃. Moreover,18 MaLAC transcripts were found to be potential targets of miR397 or miR408.

Key words: banana, Laccase family, member identification, bioinformatics analysis, low temperature stress

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